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Prenylated phenolics from Morus alba against MRSA infections as a strategy for wound healing

Antimicrobial resistance is a public health threat and the increasing number of multidrug-resistant bacteria is a major concern worldwide. Common antibiotics are becoming ineffective for skin infections and wounds, making the search for new therapeutic options increasingly urgent. The present study...

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Autores principales: Škovranová, Gabriela, Čulenová, Marie, Treml, Jakub, Dzurická, Lucia, Marova, Ivana, Sychrová, Alice
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9747775/
https://www.ncbi.nlm.nih.gov/pubmed/36532741
http://dx.doi.org/10.3389/fphar.2022.1068371
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author Škovranová, Gabriela
Čulenová, Marie
Treml, Jakub
Dzurická, Lucia
Marova, Ivana
Sychrová, Alice
author_facet Škovranová, Gabriela
Čulenová, Marie
Treml, Jakub
Dzurická, Lucia
Marova, Ivana
Sychrová, Alice
author_sort Škovranová, Gabriela
collection PubMed
description Antimicrobial resistance is a public health threat and the increasing number of multidrug-resistant bacteria is a major concern worldwide. Common antibiotics are becoming ineffective for skin infections and wounds, making the search for new therapeutic options increasingly urgent. The present study aimed to investigate the antibacterial potential of prenylated phenolics in wound healing. Phenolic compounds isolated from the root bark of Morus alba L. were investigated for their antistaphylococcal potential both alone and in combination with commonly used antibiotics. The minimum inhibitory concentration (MIC) and the minimum bactericidal concentration (MBC) were determined by microdilution and agar method. Synergy was investigated using the checkerboard titration technique. Membrane-disrupting activity and efflux pump inhibition were evaluated to describe the potentiating effect. Prenylated phenolics inhibited bacterial growth of methicillin-resistant Staphylococcus aureus (MRSA) at lower concentrations (MIC 2–8 μg/ml) than commonly used antibiotics. The combination of active phenolics with kanamycin, oxacillin, and ciprofloxacin resulted in a decrease in the MIC of the antimicrobial agent. Kuwanon C, E, T, morusin, and albafuran C showed synergy (FICi 0.375–0.5) with oxacillin and/or kanamycin. Prenylated phenolics disrupted membrane permeability statistically significantly (from 28 ± 16.48% up to 73 ± 2.83%), and membrane disruption contributes to the complex antibacterial activity against MRSA. In addition, kuwanon C could be considered an efflux pump inhibitor. Despite the antibacterial effect on MRSA and the multiple biological activities, the prenylated phenolics at microbially significant concentrations have a minor effect on human keratinocyte (HaCaT) viability. In conclusion, prenylated phenolics in combination with commonly used antibiotics are promising candidates for the treatment of MRSA infections and wound healing, although further studies are needed.
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spelling pubmed-97477752022-12-15 Prenylated phenolics from Morus alba against MRSA infections as a strategy for wound healing Škovranová, Gabriela Čulenová, Marie Treml, Jakub Dzurická, Lucia Marova, Ivana Sychrová, Alice Front Pharmacol Pharmacology Antimicrobial resistance is a public health threat and the increasing number of multidrug-resistant bacteria is a major concern worldwide. Common antibiotics are becoming ineffective for skin infections and wounds, making the search for new therapeutic options increasingly urgent. The present study aimed to investigate the antibacterial potential of prenylated phenolics in wound healing. Phenolic compounds isolated from the root bark of Morus alba L. were investigated for their antistaphylococcal potential both alone and in combination with commonly used antibiotics. The minimum inhibitory concentration (MIC) and the minimum bactericidal concentration (MBC) were determined by microdilution and agar method. Synergy was investigated using the checkerboard titration technique. Membrane-disrupting activity and efflux pump inhibition were evaluated to describe the potentiating effect. Prenylated phenolics inhibited bacterial growth of methicillin-resistant Staphylococcus aureus (MRSA) at lower concentrations (MIC 2–8 μg/ml) than commonly used antibiotics. The combination of active phenolics with kanamycin, oxacillin, and ciprofloxacin resulted in a decrease in the MIC of the antimicrobial agent. Kuwanon C, E, T, morusin, and albafuran C showed synergy (FICi 0.375–0.5) with oxacillin and/or kanamycin. Prenylated phenolics disrupted membrane permeability statistically significantly (from 28 ± 16.48% up to 73 ± 2.83%), and membrane disruption contributes to the complex antibacterial activity against MRSA. In addition, kuwanon C could be considered an efflux pump inhibitor. Despite the antibacterial effect on MRSA and the multiple biological activities, the prenylated phenolics at microbially significant concentrations have a minor effect on human keratinocyte (HaCaT) viability. In conclusion, prenylated phenolics in combination with commonly used antibiotics are promising candidates for the treatment of MRSA infections and wound healing, although further studies are needed. Frontiers Media S.A. 2022-11-30 /pmc/articles/PMC9747775/ /pubmed/36532741 http://dx.doi.org/10.3389/fphar.2022.1068371 Text en Copyright © 2022 Škovranová, Čulenová, Treml, Dzurická, Marova and Sychrová. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Pharmacology
Škovranová, Gabriela
Čulenová, Marie
Treml, Jakub
Dzurická, Lucia
Marova, Ivana
Sychrová, Alice
Prenylated phenolics from Morus alba against MRSA infections as a strategy for wound healing
title Prenylated phenolics from Morus alba against MRSA infections as a strategy for wound healing
title_full Prenylated phenolics from Morus alba against MRSA infections as a strategy for wound healing
title_fullStr Prenylated phenolics from Morus alba against MRSA infections as a strategy for wound healing
title_full_unstemmed Prenylated phenolics from Morus alba against MRSA infections as a strategy for wound healing
title_short Prenylated phenolics from Morus alba against MRSA infections as a strategy for wound healing
title_sort prenylated phenolics from morus alba against mrsa infections as a strategy for wound healing
topic Pharmacology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9747775/
https://www.ncbi.nlm.nih.gov/pubmed/36532741
http://dx.doi.org/10.3389/fphar.2022.1068371
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