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Efficient simultaneous double DNA knock-in in murine embryonic stem cells by CRISPR/Cas9 ribonucleoprotein-mediated circular plasmid targeting for generating gene-manipulated mice

Gene targeting of embryonic stem (ES) cells followed by chimera production has been conventionally used for developing gene-manipulated mice. Although direct knock-in (KI) using murine zygote via CRISPR/Cas9-mediated genome editing has been reported, ES cell targeting still has merits, e.g., high th...

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Detalles Bibliográficos
Autores principales:	Ozawa, Manabu, Taguchi, Jumpei, Katsuma, Kento, Ishikawa-Yamauchi, Yu, Kikuchi, Mio, Sakamoto, Reiko, Yamada, Yasuhiro, Ikawa, Masahito
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Nature Publishing Group UK 2022
Materias:	Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9748034/ https://www.ncbi.nlm.nih.gov/pubmed/36513736 http://dx.doi.org/10.1038/s41598-022-26107-z

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