Cargando…

Efficient simultaneous double DNA knock-in in murine embryonic stem cells by CRISPR/Cas9 ribonucleoprotein-mediated circular plasmid targeting for generating gene-manipulated mice

Gene targeting of embryonic stem (ES) cells followed by chimera production has been conventionally used for developing gene-manipulated mice. Although direct knock-in (KI) using murine zygote via CRISPR/Cas9-mediated genome editing has been reported, ES cell targeting still has merits, e.g., high th...

Descripción completa

Detalles Bibliográficos
Autores principales:	Ozawa, Manabu, Taguchi, Jumpei, Katsuma, Kento, Ishikawa-Yamauchi, Yu, Kikuchi, Mio, Sakamoto, Reiko, Yamada, Yasuhiro, Ikawa, Masahito
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Nature Publishing Group UK 2022
Materias:	Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9748034/ https://www.ncbi.nlm.nih.gov/pubmed/36513736 http://dx.doi.org/10.1038/s41598-022-26107-z

Ejemplares similares

Targeted gene knock-in by CRISPR/Cas ribonucleoproteins in porcine zygotes
por: Park, Ki-Eun, et al.
Publicado: (2017)

Generation of mutant mice by pronuclear injection of circular plasmid expressing Cas9 and single guided RNA
por: Mashiko, Daisuke, et al.
Publicado: (2013)

RNA-binding protein Ptbp1 regulates alternative splicing and transcriptome in spermatogonia and maintains spermatogenesis in concert with Nanos3
por: SENOO, Manami, et al.
Publicado: (2020)

Efficient Generation of Knock-In Zebrafish Models for Inherited Disorders Using CRISPR-Cas9 Ribonucleoprotein Complexes
por: de Vrieze, Erik, et al.
Publicado: (2021)

Knocking out consumer concerns and regulator’s rules: efficient use of CRISPR/Cas ribonucleoprotein complexes for genome editing in cereals
por: Wolter, Felix, et al.
Publicado: (2017)

Highly improved cloning efficiency for plasmid-based CRISPR knock-in in C. elegans
por: DeMott, Ella, et al.
Publicado: (2021)

Expanding the toolkit for genetic manipulation and discovery in Candida species using a CRISPR ribonucleoprotein-based approach
por: Gregor, Justin B., et al.
Publicado: (2023)

PTBP1 contributes to spermatogenesis through regulation of proliferation in spermatogonia
por: SENOO, Manami, et al.
Publicado: (2018)

Targeted gene knock-in by homology-directed genome editing using Cas9 ribonucleoprotein and AAV donor delivery
por: Gaj, Thomas, et al.
Publicado: (2017)

Efficient multiple gene knockout in Colletotrichum higginsianum via CRISPR/Cas9 ribonucleoprotein and URA3‐based marker recycling
por: Yonehara, Katsuma, et al.
Publicado: (2023)

High-frequency random DNA insertions upon co-delivery of CRISPR-Cas9 ribonucleoprotein and selectable marker plasmid in rice
por: Banakar, Raviraj, et al.
Publicado: (2019)

An analytical platform for mass spectrometry-based identification and chemical analysis of RNA in ribonucleoprotein complexes
por: Taoka, Masato, et al.
Publicado: (2009)

Tools of the trade: plasmid repositories and standardized plasmid manipulation for molecular and synthetic biology
por: Dolan, Stephen K., et al.
Publicado: (2022)

Combi-CRISPR: combination of NHEJ and HDR provides efficient and precise plasmid-based knock-ins in mice and rats
por: Yoshimi, Kazuto, et al.
Publicado: (2020)

Efficient derivation of knock-out and knock-in rats using embryos obtained by in vitro fertilization
por: Honda, Arata, et al.
Publicado: (2019)

CRISPR/Cas9-mediated knock-in of the murine Y chromosomal Sry gene
por: IMAIMATSU, Kenya, et al.
Publicado: (2018)

Electronic Circular Dichroism of the Cas9 Protein and gRNA:Cas9 Ribonucleoprotein Complex
por: Halat, Monika, et al.
Publicado: (2021)

CRISPR ribonucleoprotein-mediated genetic engineering in plants
por: Zhang, Yingxiao, et al.
Publicado: (2021)

A selectable, plasmid-based system to generate CRISPR/Cas9 gene edited and knock-in mosquito cell lines
por: Rozen-Gagnon, Kathryn, et al.
Publicado: (2021)

mScarlet and split fluorophore mScarlet resources for plasmid-based CRISPR/Cas9 knock-in in C. elegans
por: Witten, Gillian, et al.
Publicado: (2023)

Author Correction: Efficient derivation of knock-out and knock-in rats using embryos obtained by in vitro fertilization
por: Honda, Arata, et al.
Publicado: (2020)

Why do plasmids manipulate the expression of bacterial phenotypes?
por: Billane, Kathryn, et al.
Publicado: (2022)

High-resolution analysis of baculovirus-induced host manipulation in the domestic silkworm, Bombyx mori
por: Hikida, Hiroyuki, et al.
Publicado: (2021)

DGKγ Knock-Out Mice Show Impairments in Cerebellar Motor Coordination, LTD, and the Dendritic Development of Purkinje Cells through the Activation of PKCγ
por: Tsumagari, Ryosuke, et al.
Publicado: (2020)

Plasmids manipulate bacterial behaviour through translational regulatory crosstalk
por: Thompson, Catriona M. A., et al.
Publicado: (2023)

Structure of spliceosomal ribonucleoproteins
por: Krummel, Daniel Aaron Pomeranz, et al.
Publicado: (2010)

The Ribonucleoprotein Csr Network
por: Seyll, Ethel, et al.
Publicado: (2013)

Author Correction: A selectable, plasmid-based system to generate CRISPR/Cas9 gene edited and knock-in mosquito cell lines
por: Rozen-Gagnon, Kathryn, et al.
Publicado: (2021)

Knock-Down of Heterogeneous Nuclear Ribonucleoprotein A1 Results in Neurite Damage, Altered Stress Granule Biology, and Cellular Toxicity in Differentiated Neuronal Cells
por: Anees, Amber, et al.
Publicado: (2021)

High-resolution analysis of baculovirus-induced host manipulation in the domestic silkworm, Bombyx mori – CORRIGENDUM
por: Hikida, Hiroyuki, et al.
Publicado: (2021)

Structure of a type IV CRISPR-Cas ribonucleoprotein complex
por: Zhou, Yi, et al.
Publicado: (2021)

Engineering extracellular vesicles to deliver CRISPR ribonucleoprotein for gene editing
por: Whitley, Joseph Andrew, et al.
Publicado: (2023)

Manipulation of hadron beams with bent crystals in circular accelerators
por: Rossi, R, et al.
Publicado: (2016)

Recent Progress on Nonlinear Beam Manipulations in Circular Accelerators
por: Bazzani, Armando, et al.
Publicado: (2022)

Structure of the IscB–ωRNA ribonucleoprotein complex, the likely ancestor of CRISPR-Cas9
por: Kato, Kazuki, et al.
Publicado: (2022)

Highly efficient transgene‐free targeted mutagenesis and single‐stranded oligodeoxynucleotide‐mediated precise knock‐in in the industrial microalga Euglena gracilis using Cas9 ribonucleoproteins
por: Nomura, Toshihisa, et al.
Publicado: (2019)

Selecting for CRISPR-Edited Knock-In Cells
por: Reuven, Nina, et al.
Publicado: (2022)

Ribonucleoprotein particles in cellular processes
Publicado: (1988)

The Nucleolus and the Four Ribonucleoproteins of Translation
por: Pederson, Thoru, et al.
Publicado: (2000)

Ribonucleoprotein Therapy in Alzheimer's Disease?
por: Yoon, Je-Hyun, et al.
Publicado: (2014)

Cannot write session to /tmp/vufind_sessions/sess_veje5v2pvt5b9og0h5p5bnpqbr