Cargando…
Allium sativum@AgNPs and Phyllanthus urinaria@AgNPs: a comparative analysis for antibacterial application
Although medicinal herbs contain many biologically active ingredients that can act as antibiotic agents, most of them are difficult to dissolve in lipids and absorb through biofilms in the gastrointestinal tract. Besides, silver nanoparticles (AgNPs) have been widely used as a potential antibacteria...
Autores principales: | , , , , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
The Royal Society of Chemistry
2022
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9748653/ https://www.ncbi.nlm.nih.gov/pubmed/36545079 http://dx.doi.org/10.1039/d2ra06847h |
_version_ | 1784849869343555584 |
---|---|
author | Phan, Ke Son Nguyen, Thi Minh To, Xuan Thang Le, Thi Thu Huong Nguyen, Thanh Trung Pham, Kim Dang Hoang, Phuong Ha Dong, Thi Nham Dang, Dinh Kim Phan, Thi Hong Tuyet Mai, Thi Thu Trang Ha, Phuong Thu |
author_facet | Phan, Ke Son Nguyen, Thi Minh To, Xuan Thang Le, Thi Thu Huong Nguyen, Thanh Trung Pham, Kim Dang Hoang, Phuong Ha Dong, Thi Nham Dang, Dinh Kim Phan, Thi Hong Tuyet Mai, Thi Thu Trang Ha, Phuong Thu |
author_sort | Phan, Ke Son |
collection | PubMed |
description | Although medicinal herbs contain many biologically active ingredients that can act as antibiotic agents, most of them are difficult to dissolve in lipids and absorb through biofilms in the gastrointestinal tract. Besides, silver nanoparticles (AgNPs) have been widely used as a potential antibacterial agent, however, to achieve a bactericidal effect, high concentrations are required. In this work, AgNPs were combined into plant-based antibiotic nanoemulsions using biocompatible alginate/carboxyl methylcellulose scaffolds. The silver nanoparticles were prepared by a green method with an aqueous extract of Allium sativum or Phyllanthus urinaria extract. The botanical antibiotic components in the alcoholic extract of these plants were encapsulated with emulsifier poloxamer 407 to reduce the particle size, and make the active ingredients both water-soluble and lipid-soluble. Field emission scanning electron microscopy (FESEM) and energy-dispersive X-ray (EDX) analysis showed that the prepared nanosystems were spherical with a size of about 20 nm. Fourier transform infrared spectroscopy (FTIR) confirmed the interaction of the extracts and the alginate/carboxyl methylcellulose carrier. In vitro drug release kinetics of allicin and phyllanthin from the nanosystems exhibited a retarded release under different biological pH conditions. The antimicrobial activity of the synthesized nanoformulations were tested against Escherichia coli. The results showed that the nanosystem based on Allium sativum possesses a significantly higher antimicrobial activity against the tested organisms. Therefore, the combination of AgNPs with active compounds from Allium sativum extract is a good candidate for in vivo infection treatment application. |
format | Online Article Text |
id | pubmed-9748653 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | The Royal Society of Chemistry |
record_format | MEDLINE/PubMed |
spelling | pubmed-97486532022-12-20 Allium sativum@AgNPs and Phyllanthus urinaria@AgNPs: a comparative analysis for antibacterial application Phan, Ke Son Nguyen, Thi Minh To, Xuan Thang Le, Thi Thu Huong Nguyen, Thanh Trung Pham, Kim Dang Hoang, Phuong Ha Dong, Thi Nham Dang, Dinh Kim Phan, Thi Hong Tuyet Mai, Thi Thu Trang Ha, Phuong Thu RSC Adv Chemistry Although medicinal herbs contain many biologically active ingredients that can act as antibiotic agents, most of them are difficult to dissolve in lipids and absorb through biofilms in the gastrointestinal tract. Besides, silver nanoparticles (AgNPs) have been widely used as a potential antibacterial agent, however, to achieve a bactericidal effect, high concentrations are required. In this work, AgNPs were combined into plant-based antibiotic nanoemulsions using biocompatible alginate/carboxyl methylcellulose scaffolds. The silver nanoparticles were prepared by a green method with an aqueous extract of Allium sativum or Phyllanthus urinaria extract. The botanical antibiotic components in the alcoholic extract of these plants were encapsulated with emulsifier poloxamer 407 to reduce the particle size, and make the active ingredients both water-soluble and lipid-soluble. Field emission scanning electron microscopy (FESEM) and energy-dispersive X-ray (EDX) analysis showed that the prepared nanosystems were spherical with a size of about 20 nm. Fourier transform infrared spectroscopy (FTIR) confirmed the interaction of the extracts and the alginate/carboxyl methylcellulose carrier. In vitro drug release kinetics of allicin and phyllanthin from the nanosystems exhibited a retarded release under different biological pH conditions. The antimicrobial activity of the synthesized nanoformulations were tested against Escherichia coli. The results showed that the nanosystem based on Allium sativum possesses a significantly higher antimicrobial activity against the tested organisms. Therefore, the combination of AgNPs with active compounds from Allium sativum extract is a good candidate for in vivo infection treatment application. The Royal Society of Chemistry 2022-12-14 /pmc/articles/PMC9748653/ /pubmed/36545079 http://dx.doi.org/10.1039/d2ra06847h Text en This journal is © The Royal Society of Chemistry https://creativecommons.org/licenses/by-nc/3.0/ |
spellingShingle | Chemistry Phan, Ke Son Nguyen, Thi Minh To, Xuan Thang Le, Thi Thu Huong Nguyen, Thanh Trung Pham, Kim Dang Hoang, Phuong Ha Dong, Thi Nham Dang, Dinh Kim Phan, Thi Hong Tuyet Mai, Thi Thu Trang Ha, Phuong Thu Allium sativum@AgNPs and Phyllanthus urinaria@AgNPs: a comparative analysis for antibacterial application |
title |
Allium sativum@AgNPs and Phyllanthus urinaria@AgNPs: a comparative analysis for antibacterial application |
title_full |
Allium sativum@AgNPs and Phyllanthus urinaria@AgNPs: a comparative analysis for antibacterial application |
title_fullStr |
Allium sativum@AgNPs and Phyllanthus urinaria@AgNPs: a comparative analysis for antibacterial application |
title_full_unstemmed |
Allium sativum@AgNPs and Phyllanthus urinaria@AgNPs: a comparative analysis for antibacterial application |
title_short |
Allium sativum@AgNPs and Phyllanthus urinaria@AgNPs: a comparative analysis for antibacterial application |
title_sort | allium sativum@agnps and phyllanthus urinaria@agnps: a comparative analysis for antibacterial application |
topic | Chemistry |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9748653/ https://www.ncbi.nlm.nih.gov/pubmed/36545079 http://dx.doi.org/10.1039/d2ra06847h |
work_keys_str_mv | AT phankeson alliumsativumagnpsandphyllanthusurinariaagnpsacomparativeanalysisforantibacterialapplication AT nguyenthiminh alliumsativumagnpsandphyllanthusurinariaagnpsacomparativeanalysisforantibacterialapplication AT toxuanthang alliumsativumagnpsandphyllanthusurinariaagnpsacomparativeanalysisforantibacterialapplication AT lethithuhuong alliumsativumagnpsandphyllanthusurinariaagnpsacomparativeanalysisforantibacterialapplication AT nguyenthanhtrung alliumsativumagnpsandphyllanthusurinariaagnpsacomparativeanalysisforantibacterialapplication AT phamkimdang alliumsativumagnpsandphyllanthusurinariaagnpsacomparativeanalysisforantibacterialapplication AT hoangphuongha alliumsativumagnpsandphyllanthusurinariaagnpsacomparativeanalysisforantibacterialapplication AT dongthinham alliumsativumagnpsandphyllanthusurinariaagnpsacomparativeanalysisforantibacterialapplication AT dangdinhkim alliumsativumagnpsandphyllanthusurinariaagnpsacomparativeanalysisforantibacterialapplication AT phanthihongtuyet alliumsativumagnpsandphyllanthusurinariaagnpsacomparativeanalysisforantibacterialapplication AT maithithutrang alliumsativumagnpsandphyllanthusurinariaagnpsacomparativeanalysisforantibacterialapplication AT haphuongthu alliumsativumagnpsandphyllanthusurinariaagnpsacomparativeanalysisforantibacterialapplication |