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Metformin modulates the gut microbiome in a mice model of high-fat diet-induced glycolipid metabolism disorder
INTRODUCTION: Metformin (MET) can regulate glucose and lipid levels, and the gut microbiota may be involved in the control of metabolism. We hypothesized that MET alleviates glucolipid metabolism disorder by modulating gut microbiota and microbial metabolites. RESEARCH DESIGN AND METHODS: A total of...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BMJ Publishing Group
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9748938/ https://www.ncbi.nlm.nih.gov/pubmed/36593662 http://dx.doi.org/10.1136/bmjdrc-2022-003149 |
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author | Wu, Haoran Wang, Xinmiao Fang, Xinyi Lian, Fengmei Li, Min Liao, Jiangquan Dai, Dan Tian, Jiaxing |
author_facet | Wu, Haoran Wang, Xinmiao Fang, Xinyi Lian, Fengmei Li, Min Liao, Jiangquan Dai, Dan Tian, Jiaxing |
author_sort | Wu, Haoran |
collection | PubMed |
description | INTRODUCTION: Metformin (MET) can regulate glucose and lipid levels, and the gut microbiota may be involved in the control of metabolism. We hypothesized that MET alleviates glucolipid metabolism disorder by modulating gut microbiota and microbial metabolites. RESEARCH DESIGN AND METHODS: A total of 24 male C57BL/6 J mice were equally divided into three groups (normal control, model control (MC), and MET-treated groups). Model mice were established by feeding a high-fat diet for 6 weeks. The MET-treated group was administered MET solution (2.5 g/100 mL, 250 mg/kg). Fecal samples were collected to characterize the microbiota system using metagenomic shotgun sequencing and gas chromatography–time of flight–mass spectrometry analysis. Phenotypic and biochemical indices were obtained for further correlation analysis. RESULTS: Compared with the MC group, MET reduced the levels of weight, glucose, areas under the glucose curve in the glucose tolerance test, triglyceride (TG), and total cholesterol (TC). A decreasing abundance of bacteria, including Parabacteroides distasonis, and an increasing abundance of bacteria, including Bacteroides vulgatus, were observed in the MET-treated group. The 2-deoxytetronic acid declined after MET intervention and was positively correlated with species over-represented in the MC group and negatively correlated with species enriched in the MET-treated group. Additionally, species enriched in the MET-treated group negatively correlated with glucose, areas under the glucose curve in the glucose tolerance test, and TGs. Further, the correlation between the differential metabolites, which decreased after MET intervention, and the phenotypic indices was positive. CONCLUSIONS: MET-induced restoration of intestinal homeostasis correlates with the amelioration of host glucolipid metabolism. |
format | Online Article Text |
id | pubmed-9748938 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | BMJ Publishing Group |
record_format | MEDLINE/PubMed |
spelling | pubmed-97489382022-12-15 Metformin modulates the gut microbiome in a mice model of high-fat diet-induced glycolipid metabolism disorder Wu, Haoran Wang, Xinmiao Fang, Xinyi Lian, Fengmei Li, Min Liao, Jiangquan Dai, Dan Tian, Jiaxing BMJ Open Diabetes Res Care Genetics/Genomes/Proteomics/Metabolomics INTRODUCTION: Metformin (MET) can regulate glucose and lipid levels, and the gut microbiota may be involved in the control of metabolism. We hypothesized that MET alleviates glucolipid metabolism disorder by modulating gut microbiota and microbial metabolites. RESEARCH DESIGN AND METHODS: A total of 24 male C57BL/6 J mice were equally divided into three groups (normal control, model control (MC), and MET-treated groups). Model mice were established by feeding a high-fat diet for 6 weeks. The MET-treated group was administered MET solution (2.5 g/100 mL, 250 mg/kg). Fecal samples were collected to characterize the microbiota system using metagenomic shotgun sequencing and gas chromatography–time of flight–mass spectrometry analysis. Phenotypic and biochemical indices were obtained for further correlation analysis. RESULTS: Compared with the MC group, MET reduced the levels of weight, glucose, areas under the glucose curve in the glucose tolerance test, triglyceride (TG), and total cholesterol (TC). A decreasing abundance of bacteria, including Parabacteroides distasonis, and an increasing abundance of bacteria, including Bacteroides vulgatus, were observed in the MET-treated group. The 2-deoxytetronic acid declined after MET intervention and was positively correlated with species over-represented in the MC group and negatively correlated with species enriched in the MET-treated group. Additionally, species enriched in the MET-treated group negatively correlated with glucose, areas under the glucose curve in the glucose tolerance test, and TGs. Further, the correlation between the differential metabolites, which decreased after MET intervention, and the phenotypic indices was positive. CONCLUSIONS: MET-induced restoration of intestinal homeostasis correlates with the amelioration of host glucolipid metabolism. BMJ Publishing Group 2022-12-13 /pmc/articles/PMC9748938/ /pubmed/36593662 http://dx.doi.org/10.1136/bmjdrc-2022-003149 Text en © Author(s) (or their employer(s)) 2022. Re-use permitted under CC BY-NC. No commercial re-use. See rights and permissions. Published by BMJ. https://creativecommons.org/licenses/by-nc/4.0/This is an open access article distributed in accordance with the Creative Commons Attribution Non Commercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited, appropriate credit is given, any changes made indicated, and the use is non-commercial. See: http://creativecommons.org/licenses/by-nc/4.0/ (https://creativecommons.org/licenses/by-nc/4.0/) . |
spellingShingle | Genetics/Genomes/Proteomics/Metabolomics Wu, Haoran Wang, Xinmiao Fang, Xinyi Lian, Fengmei Li, Min Liao, Jiangquan Dai, Dan Tian, Jiaxing Metformin modulates the gut microbiome in a mice model of high-fat diet-induced glycolipid metabolism disorder |
title | Metformin modulates the gut microbiome in a mice model of high-fat diet-induced glycolipid metabolism disorder |
title_full | Metformin modulates the gut microbiome in a mice model of high-fat diet-induced glycolipid metabolism disorder |
title_fullStr | Metformin modulates the gut microbiome in a mice model of high-fat diet-induced glycolipid metabolism disorder |
title_full_unstemmed | Metformin modulates the gut microbiome in a mice model of high-fat diet-induced glycolipid metabolism disorder |
title_short | Metformin modulates the gut microbiome in a mice model of high-fat diet-induced glycolipid metabolism disorder |
title_sort | metformin modulates the gut microbiome in a mice model of high-fat diet-induced glycolipid metabolism disorder |
topic | Genetics/Genomes/Proteomics/Metabolomics |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9748938/ https://www.ncbi.nlm.nih.gov/pubmed/36593662 http://dx.doi.org/10.1136/bmjdrc-2022-003149 |
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