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Expression of poly(ADP-ribose) polymerase-1 gene and optical coherence tomography angiographic parameters among patients with multiple sclerosis
BACK GROUND/AIMS: To analyse different parameters of the macula, disc and their vascular affection using optical coherence tomography (OCT) and angiography (OCT-A) in patients with multiple sclerosis (MS) correlating these changes to PARP-1 gene expression in blood. METHODS: This cross-sectional stu...
Autores principales: | , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BMJ Publishing Group
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9748967/ http://dx.doi.org/10.1136/bmjophth-2022-001157 |
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author | Mossa, Elshimaa A Mateen Sayed, Khulood Muhammad Awny, Islam Mohamed, Nesma A Ali, Tasneem Hemdan, Shimaa Badawy Helaly, Ahmed A Abdellatif, Mohammed G Farag, Radwa Mohammed Alsmman, Alahmady Hamad Mounir, Amr |
author_facet | Mossa, Elshimaa A Mateen Sayed, Khulood Muhammad Awny, Islam Mohamed, Nesma A Ali, Tasneem Hemdan, Shimaa Badawy Helaly, Ahmed A Abdellatif, Mohammed G Farag, Radwa Mohammed Alsmman, Alahmady Hamad Mounir, Amr |
author_sort | Mossa, Elshimaa A Mateen |
collection | PubMed |
description | BACK GROUND/AIMS: To analyse different parameters of the macula, disc and their vascular affection using optical coherence tomography (OCT) and angiography (OCT-A) in patients with multiple sclerosis (MS) correlating these changes to PARP-1 gene expression in blood. METHODS: This cross-sectional study included 80 eyes of the clinically diagnosed relapsing-remitting phenotype of MS. The study included three groups; group (A) included 40 eyes of 20 patients with MS with a history of optic neuritis (MS+ON), group (B) included 40 eyes of 20 patients with MS without a history of ON (MS-ON) and group (C) (the control group) consisted of 40 eyes of 20 matched participants not suffering from any ocular or systemic disease. OCT and OCT-A, RTVue (Optovue, Fermont, California, USA) were done for all eyes for evaluating the macular and disc changes. Qualitative real-time PCR for estimation of PARP1 gene expression level was performed for all patients. RESULTS: PARP-1 gene expression level showed a significant difference in comparing the three groups, with the highest level being for the (ON+) group (p<0.0009). Significant negative correlations were found between PARP-1 gene expression level and central macular thickness, total macular volume and full foveal vessel density thickness. ROC curve constructed by plotting the area under the receiver operating characteristic curve value was (0.9) for PARP-1 gene expression level. CONCLUSIONS: PARP-1 may play an important role in the development of the ON cascade in patients with MS and may be a biomarker for diagnosing and a potential molecular target of ON in MS patients’ therapy. In addition to the OCT and OCT-angio changes that could be detected retrospectively, PARP-1 gene expression level could be considered a prospective detector to complete the full-blown picture of MS (ON+) early and prevent blindness. |
format | Online Article Text |
id | pubmed-9748967 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | BMJ Publishing Group |
record_format | MEDLINE/PubMed |
spelling | pubmed-97489672022-12-15 Expression of poly(ADP-ribose) polymerase-1 gene and optical coherence tomography angiographic parameters among patients with multiple sclerosis Mossa, Elshimaa A Mateen Sayed, Khulood Muhammad Awny, Islam Mohamed, Nesma A Ali, Tasneem Hemdan, Shimaa Badawy Helaly, Ahmed A Abdellatif, Mohammed G Farag, Radwa Mohammed Alsmman, Alahmady Hamad Mounir, Amr BMJ Open Ophthalmol Neuro-Ophthalmology BACK GROUND/AIMS: To analyse different parameters of the macula, disc and their vascular affection using optical coherence tomography (OCT) and angiography (OCT-A) in patients with multiple sclerosis (MS) correlating these changes to PARP-1 gene expression in blood. METHODS: This cross-sectional study included 80 eyes of the clinically diagnosed relapsing-remitting phenotype of MS. The study included three groups; group (A) included 40 eyes of 20 patients with MS with a history of optic neuritis (MS+ON), group (B) included 40 eyes of 20 patients with MS without a history of ON (MS-ON) and group (C) (the control group) consisted of 40 eyes of 20 matched participants not suffering from any ocular or systemic disease. OCT and OCT-A, RTVue (Optovue, Fermont, California, USA) were done for all eyes for evaluating the macular and disc changes. Qualitative real-time PCR for estimation of PARP1 gene expression level was performed for all patients. RESULTS: PARP-1 gene expression level showed a significant difference in comparing the three groups, with the highest level being for the (ON+) group (p<0.0009). Significant negative correlations were found between PARP-1 gene expression level and central macular thickness, total macular volume and full foveal vessel density thickness. ROC curve constructed by plotting the area under the receiver operating characteristic curve value was (0.9) for PARP-1 gene expression level. CONCLUSIONS: PARP-1 may play an important role in the development of the ON cascade in patients with MS and may be a biomarker for diagnosing and a potential molecular target of ON in MS patients’ therapy. In addition to the OCT and OCT-angio changes that could be detected retrospectively, PARP-1 gene expression level could be considered a prospective detector to complete the full-blown picture of MS (ON+) early and prevent blindness. BMJ Publishing Group 2022-12-13 /pmc/articles/PMC9748967/ http://dx.doi.org/10.1136/bmjophth-2022-001157 Text en © Author(s) (or their employer(s)) 2022. Re-use permitted under CC BY-NC. No commercial re-use. See rights and permissions. Published by BMJ. https://creativecommons.org/licenses/by-nc/4.0/This is an open access article distributed in accordance with the Creative Commons Attribution Non Commercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited, appropriate credit is given, any changes made indicated, and the use is non-commercial. See: http://creativecommons.org/licenses/by-nc/4.0/ (https://creativecommons.org/licenses/by-nc/4.0/) . |
spellingShingle | Neuro-Ophthalmology Mossa, Elshimaa A Mateen Sayed, Khulood Muhammad Awny, Islam Mohamed, Nesma A Ali, Tasneem Hemdan, Shimaa Badawy Helaly, Ahmed A Abdellatif, Mohammed G Farag, Radwa Mohammed Alsmman, Alahmady Hamad Mounir, Amr Expression of poly(ADP-ribose) polymerase-1 gene and optical coherence tomography angiographic parameters among patients with multiple sclerosis |
title | Expression of poly(ADP-ribose) polymerase-1 gene and optical coherence tomography angiographic parameters among patients with multiple sclerosis |
title_full | Expression of poly(ADP-ribose) polymerase-1 gene and optical coherence tomography angiographic parameters among patients with multiple sclerosis |
title_fullStr | Expression of poly(ADP-ribose) polymerase-1 gene and optical coherence tomography angiographic parameters among patients with multiple sclerosis |
title_full_unstemmed | Expression of poly(ADP-ribose) polymerase-1 gene and optical coherence tomography angiographic parameters among patients with multiple sclerosis |
title_short | Expression of poly(ADP-ribose) polymerase-1 gene and optical coherence tomography angiographic parameters among patients with multiple sclerosis |
title_sort | expression of poly(adp-ribose) polymerase-1 gene and optical coherence tomography angiographic parameters among patients with multiple sclerosis |
topic | Neuro-Ophthalmology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9748967/ http://dx.doi.org/10.1136/bmjophth-2022-001157 |
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