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Expression of poly(ADP-ribose) polymerase-1 gene and optical coherence tomography angiographic parameters among patients with multiple sclerosis

BACK GROUND/AIMS: To analyse different parameters of the macula, disc and their vascular affection using optical coherence tomography (OCT) and angiography (OCT-A) in patients with multiple sclerosis (MS) correlating these changes to PARP-1 gene expression in blood. METHODS: This cross-sectional stu...

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Autores principales: Mossa, Elshimaa A Mateen, Sayed, Khulood Muhammad, Awny, Islam, Mohamed, Nesma A, Ali, Tasneem, Hemdan, Shimaa Badawy, Helaly, Ahmed A, Abdellatif, Mohammed G, Farag, Radwa Mohammed, Alsmman, Alahmady Hamad, Mounir, Amr
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BMJ Publishing Group 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9748967/
http://dx.doi.org/10.1136/bmjophth-2022-001157
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author Mossa, Elshimaa A Mateen
Sayed, Khulood Muhammad
Awny, Islam
Mohamed, Nesma A
Ali, Tasneem
Hemdan, Shimaa Badawy
Helaly, Ahmed A
Abdellatif, Mohammed G
Farag, Radwa Mohammed
Alsmman, Alahmady Hamad
Mounir, Amr
author_facet Mossa, Elshimaa A Mateen
Sayed, Khulood Muhammad
Awny, Islam
Mohamed, Nesma A
Ali, Tasneem
Hemdan, Shimaa Badawy
Helaly, Ahmed A
Abdellatif, Mohammed G
Farag, Radwa Mohammed
Alsmman, Alahmady Hamad
Mounir, Amr
author_sort Mossa, Elshimaa A Mateen
collection PubMed
description BACK GROUND/AIMS: To analyse different parameters of the macula, disc and their vascular affection using optical coherence tomography (OCT) and angiography (OCT-A) in patients with multiple sclerosis (MS) correlating these changes to PARP-1 gene expression in blood. METHODS: This cross-sectional study included 80 eyes of the clinically diagnosed relapsing-remitting phenotype of MS. The study included three groups; group (A) included 40 eyes of 20 patients with MS with a history of optic neuritis (MS+ON), group (B) included 40 eyes of 20 patients with MS without a history of ON (MS-ON) and group (C) (the control group) consisted of 40 eyes of 20 matched participants not suffering from any ocular or systemic disease. OCT and OCT-A, RTVue (Optovue, Fermont, California, USA) were done for all eyes for evaluating the macular and disc changes. Qualitative real-time PCR for estimation of PARP1 gene expression level was performed for all patients. RESULTS: PARP-1 gene expression level showed a significant difference in comparing the three groups, with the highest level being for the (ON+) group (p<0.0009). Significant negative correlations were found between PARP-1 gene expression level and central macular thickness, total macular volume and full foveal vessel density thickness. ROC curve constructed by plotting the area under the receiver operating characteristic curve value was (0.9) for PARP-1 gene expression level. CONCLUSIONS: PARP-1 may play an important role in the development of the ON cascade in patients with MS and may be a biomarker for diagnosing and a potential molecular target of ON in MS patients’ therapy. In addition to the OCT and OCT-angio changes that could be detected retrospectively, PARP-1 gene expression level could be considered a prospective detector to complete the full-blown picture of MS (ON+) early and prevent blindness.
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spelling pubmed-97489672022-12-15 Expression of poly(ADP-ribose) polymerase-1 gene and optical coherence tomography angiographic parameters among patients with multiple sclerosis Mossa, Elshimaa A Mateen Sayed, Khulood Muhammad Awny, Islam Mohamed, Nesma A Ali, Tasneem Hemdan, Shimaa Badawy Helaly, Ahmed A Abdellatif, Mohammed G Farag, Radwa Mohammed Alsmman, Alahmady Hamad Mounir, Amr BMJ Open Ophthalmol Neuro-Ophthalmology BACK GROUND/AIMS: To analyse different parameters of the macula, disc and their vascular affection using optical coherence tomography (OCT) and angiography (OCT-A) in patients with multiple sclerosis (MS) correlating these changes to PARP-1 gene expression in blood. METHODS: This cross-sectional study included 80 eyes of the clinically diagnosed relapsing-remitting phenotype of MS. The study included three groups; group (A) included 40 eyes of 20 patients with MS with a history of optic neuritis (MS+ON), group (B) included 40 eyes of 20 patients with MS without a history of ON (MS-ON) and group (C) (the control group) consisted of 40 eyes of 20 matched participants not suffering from any ocular or systemic disease. OCT and OCT-A, RTVue (Optovue, Fermont, California, USA) were done for all eyes for evaluating the macular and disc changes. Qualitative real-time PCR for estimation of PARP1 gene expression level was performed for all patients. RESULTS: PARP-1 gene expression level showed a significant difference in comparing the three groups, with the highest level being for the (ON+) group (p<0.0009). Significant negative correlations were found between PARP-1 gene expression level and central macular thickness, total macular volume and full foveal vessel density thickness. ROC curve constructed by plotting the area under the receiver operating characteristic curve value was (0.9) for PARP-1 gene expression level. CONCLUSIONS: PARP-1 may play an important role in the development of the ON cascade in patients with MS and may be a biomarker for diagnosing and a potential molecular target of ON in MS patients’ therapy. In addition to the OCT and OCT-angio changes that could be detected retrospectively, PARP-1 gene expression level could be considered a prospective detector to complete the full-blown picture of MS (ON+) early and prevent blindness. BMJ Publishing Group 2022-12-13 /pmc/articles/PMC9748967/ http://dx.doi.org/10.1136/bmjophth-2022-001157 Text en © Author(s) (or their employer(s)) 2022. Re-use permitted under CC BY-NC. No commercial re-use. See rights and permissions. Published by BMJ. https://creativecommons.org/licenses/by-nc/4.0/This is an open access article distributed in accordance with the Creative Commons Attribution Non Commercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited, appropriate credit is given, any changes made indicated, and the use is non-commercial. See: http://creativecommons.org/licenses/by-nc/4.0/ (https://creativecommons.org/licenses/by-nc/4.0/) .
spellingShingle Neuro-Ophthalmology
Mossa, Elshimaa A Mateen
Sayed, Khulood Muhammad
Awny, Islam
Mohamed, Nesma A
Ali, Tasneem
Hemdan, Shimaa Badawy
Helaly, Ahmed A
Abdellatif, Mohammed G
Farag, Radwa Mohammed
Alsmman, Alahmady Hamad
Mounir, Amr
Expression of poly(ADP-ribose) polymerase-1 gene and optical coherence tomography angiographic parameters among patients with multiple sclerosis
title Expression of poly(ADP-ribose) polymerase-1 gene and optical coherence tomography angiographic parameters among patients with multiple sclerosis
title_full Expression of poly(ADP-ribose) polymerase-1 gene and optical coherence tomography angiographic parameters among patients with multiple sclerosis
title_fullStr Expression of poly(ADP-ribose) polymerase-1 gene and optical coherence tomography angiographic parameters among patients with multiple sclerosis
title_full_unstemmed Expression of poly(ADP-ribose) polymerase-1 gene and optical coherence tomography angiographic parameters among patients with multiple sclerosis
title_short Expression of poly(ADP-ribose) polymerase-1 gene and optical coherence tomography angiographic parameters among patients with multiple sclerosis
title_sort expression of poly(adp-ribose) polymerase-1 gene and optical coherence tomography angiographic parameters among patients with multiple sclerosis
topic Neuro-Ophthalmology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9748967/
http://dx.doi.org/10.1136/bmjophth-2022-001157
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