Cargando…
Embelin alleviates weaned piglets intestinal inflammation and barrier dysfunction via PCAF/NF-κB signaling pathway in intestinal epithelial cells
BACKGROUND: Intestinal barrier plays key roles in maintaining intestinal homeostasis. Inflammation damage can severely destroy the intestinal integrity of mammals. This study was conducted to investigate the protective effects of embelin and its molecular mechanisms on intestinal inflammation in a p...
Autores principales: | , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2022
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9749222/ https://www.ncbi.nlm.nih.gov/pubmed/36514139 http://dx.doi.org/10.1186/s40104-022-00787-z |
_version_ | 1784849997956644864 |
---|---|
author | Yao, Weilei Wang, Tongxin Huang, Lu Bao, Zhengxi Wen, Shu Huang, Feiruo |
author_facet | Yao, Weilei Wang, Tongxin Huang, Lu Bao, Zhengxi Wen, Shu Huang, Feiruo |
author_sort | Yao, Weilei |
collection | PubMed |
description | BACKGROUND: Intestinal barrier plays key roles in maintaining intestinal homeostasis. Inflammation damage can severely destroy the intestinal integrity of mammals. This study was conducted to investigate the protective effects of embelin and its molecular mechanisms on intestinal inflammation in a porcine model. One hundred sixty 21-day-old castrated weaned pigs (Duroc × Landrace × Yorkshire, average initial body weight was 7.05 ± 0.28 kg, equal numbers of castrated males and females) were allotted to four groups and fed with a basal diet or a basal diet containing 200, 400, or 600 mg embelin/kg for 28 d. The growth performance, intestinal inflammatory cytokines, morphology of jejunum and ileum, tight junctions in the intestinal mucosa of piglets were tested. IPEC-1 cells with overexpression of P300/CBP associating factor (PCAF) were treated with embelin, the activity of PCAF and acetylation of nuclear factor-κB (NF-κB) were analyzed to determine the effect of embelin on PCAF/NF-κB pathway in vitro. RESULTS: The results showed that embelin decreased (P < 0.05) serum D-lactate and diamine oxidase (DAO) levels, and enhanced the expression of ZO-1, occludin and claudin-1 protein in jejunum and ileum. Moreover, the expression levels of critical inflammation molecules (interleukin-1β, interleukin-6, tumor necrosis factor-α, and NF-κB) were down-regulated (P < 0.05) by embelin in jejunal and ileal mucosa. Meanwhile, the activity of PCAF were down-regulated (P < 0.05) by embelin. Importantly, transfection of PCAF siRNAs to IPEC-1 cell decreased NF-κB activities; embelin treatment downregulated (P < 0.05) the acetylation and activities of NF-κB by 31.7%–74.6% in IPEC-1 cells with overexpression of PCAF. CONCLUSIONS: These results suggested that embelin ameliorates intestinal inflammation in weaned pigs, which might be mediated by suppressing the PCAF/NF-κB signaling pathway. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s40104-022-00787-z. |
format | Online Article Text |
id | pubmed-9749222 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-97492222022-12-15 Embelin alleviates weaned piglets intestinal inflammation and barrier dysfunction via PCAF/NF-κB signaling pathway in intestinal epithelial cells Yao, Weilei Wang, Tongxin Huang, Lu Bao, Zhengxi Wen, Shu Huang, Feiruo J Anim Sci Biotechnol Research BACKGROUND: Intestinal barrier plays key roles in maintaining intestinal homeostasis. Inflammation damage can severely destroy the intestinal integrity of mammals. This study was conducted to investigate the protective effects of embelin and its molecular mechanisms on intestinal inflammation in a porcine model. One hundred sixty 21-day-old castrated weaned pigs (Duroc × Landrace × Yorkshire, average initial body weight was 7.05 ± 0.28 kg, equal numbers of castrated males and females) were allotted to four groups and fed with a basal diet or a basal diet containing 200, 400, or 600 mg embelin/kg for 28 d. The growth performance, intestinal inflammatory cytokines, morphology of jejunum and ileum, tight junctions in the intestinal mucosa of piglets were tested. IPEC-1 cells with overexpression of P300/CBP associating factor (PCAF) were treated with embelin, the activity of PCAF and acetylation of nuclear factor-κB (NF-κB) were analyzed to determine the effect of embelin on PCAF/NF-κB pathway in vitro. RESULTS: The results showed that embelin decreased (P < 0.05) serum D-lactate and diamine oxidase (DAO) levels, and enhanced the expression of ZO-1, occludin and claudin-1 protein in jejunum and ileum. Moreover, the expression levels of critical inflammation molecules (interleukin-1β, interleukin-6, tumor necrosis factor-α, and NF-κB) were down-regulated (P < 0.05) by embelin in jejunal and ileal mucosa. Meanwhile, the activity of PCAF were down-regulated (P < 0.05) by embelin. Importantly, transfection of PCAF siRNAs to IPEC-1 cell decreased NF-κB activities; embelin treatment downregulated (P < 0.05) the acetylation and activities of NF-κB by 31.7%–74.6% in IPEC-1 cells with overexpression of PCAF. CONCLUSIONS: These results suggested that embelin ameliorates intestinal inflammation in weaned pigs, which might be mediated by suppressing the PCAF/NF-κB signaling pathway. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s40104-022-00787-z. BioMed Central 2022-12-14 /pmc/articles/PMC9749222/ /pubmed/36514139 http://dx.doi.org/10.1186/s40104-022-00787-z Text en © The Author(s) 2022 https://creativecommons.org/licenses/by/4.0/Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/ (https://creativecommons.org/publicdomain/zero/1.0/) ) applies to the data made available in this article, unless otherwise stated in a credit line to the data. |
spellingShingle | Research Yao, Weilei Wang, Tongxin Huang, Lu Bao, Zhengxi Wen, Shu Huang, Feiruo Embelin alleviates weaned piglets intestinal inflammation and barrier dysfunction via PCAF/NF-κB signaling pathway in intestinal epithelial cells |
title | Embelin alleviates weaned piglets intestinal inflammation and barrier dysfunction via PCAF/NF-κB signaling pathway in intestinal epithelial cells |
title_full | Embelin alleviates weaned piglets intestinal inflammation and barrier dysfunction via PCAF/NF-κB signaling pathway in intestinal epithelial cells |
title_fullStr | Embelin alleviates weaned piglets intestinal inflammation and barrier dysfunction via PCAF/NF-κB signaling pathway in intestinal epithelial cells |
title_full_unstemmed | Embelin alleviates weaned piglets intestinal inflammation and barrier dysfunction via PCAF/NF-κB signaling pathway in intestinal epithelial cells |
title_short | Embelin alleviates weaned piglets intestinal inflammation and barrier dysfunction via PCAF/NF-κB signaling pathway in intestinal epithelial cells |
title_sort | embelin alleviates weaned piglets intestinal inflammation and barrier dysfunction via pcaf/nf-κb signaling pathway in intestinal epithelial cells |
topic | Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9749222/ https://www.ncbi.nlm.nih.gov/pubmed/36514139 http://dx.doi.org/10.1186/s40104-022-00787-z |
work_keys_str_mv | AT yaoweilei embelinalleviatesweanedpigletsintestinalinflammationandbarrierdysfunctionviapcafnfkbsignalingpathwayinintestinalepithelialcells AT wangtongxin embelinalleviatesweanedpigletsintestinalinflammationandbarrierdysfunctionviapcafnfkbsignalingpathwayinintestinalepithelialcells AT huanglu embelinalleviatesweanedpigletsintestinalinflammationandbarrierdysfunctionviapcafnfkbsignalingpathwayinintestinalepithelialcells AT baozhengxi embelinalleviatesweanedpigletsintestinalinflammationandbarrierdysfunctionviapcafnfkbsignalingpathwayinintestinalepithelialcells AT wenshu embelinalleviatesweanedpigletsintestinalinflammationandbarrierdysfunctionviapcafnfkbsignalingpathwayinintestinalepithelialcells AT huangfeiruo embelinalleviatesweanedpigletsintestinalinflammationandbarrierdysfunctionviapcafnfkbsignalingpathwayinintestinalepithelialcells |