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Rouxiella badensis, a new bacterial pathogen of onion causing bulb rot

Onion bulb rot can be caused by multiple plant pathogens including bacterial pathogens. During our routine survey of commercial onion farms in 2014, 2020, and 2021, seven putative Rouxiella spp. strains were isolated from symptomatic onion samples in Georgia, United States. Upon fulfilling Koch’s po...

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Autores principales: Zhao, Mei, Tyson, Chris, Gitaitis, Ron, Kvitko, Brian, Dutta, Bhabesh
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9749400/
https://www.ncbi.nlm.nih.gov/pubmed/36532473
http://dx.doi.org/10.3389/fmicb.2022.1054813
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author Zhao, Mei
Tyson, Chris
Gitaitis, Ron
Kvitko, Brian
Dutta, Bhabesh
author_facet Zhao, Mei
Tyson, Chris
Gitaitis, Ron
Kvitko, Brian
Dutta, Bhabesh
author_sort Zhao, Mei
collection PubMed
description Onion bulb rot can be caused by multiple plant pathogens including bacterial pathogens. During our routine survey of commercial onion farms in 2014, 2020, and 2021, seven putative Rouxiella spp. strains were isolated from symptomatic onion samples in Georgia, United States. Upon fulfilling Koch’s postulates on onion, a genome analysis was conducted. Whole-genome indices (ANI and dDDH) showed that the strains belonged to Rouxiella badensis. Although the seven R. badensis strains were not pathogenic on onion foliage, the strains were able to cause bulb rot and could also produce necrotic lesions in a red onion scale assay. R. badensis populations increased significantly and to a level comparable to P. ananatis PNA 97-1R in a red onion scale infection assay. The core-genome analysis grouped all onion R. badensis strains from Georgia together, and distinct from R. badensis strains isolated from other sources and locations. Based on the genome analysis of strains (from the current study and available genomes in the repository), type I, III (Ssa-Esc and Inv-Mxi-Spa types), and V secretion systems are present in R. badensis genomes, while type II, IV, and VI secretion systems are absent. However, various secondary metabolite gene clusters were identified from R. badensis genomes, and a thiol/redox-associated enzyme gene cluster similar to the Pantoea alt cluster mediating thiosulfinate tolerance was also present in onion strains of R. badensis. This is the first report of R. badensis as a plant pathogen.
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spelling pubmed-97494002022-12-15 Rouxiella badensis, a new bacterial pathogen of onion causing bulb rot Zhao, Mei Tyson, Chris Gitaitis, Ron Kvitko, Brian Dutta, Bhabesh Front Microbiol Microbiology Onion bulb rot can be caused by multiple plant pathogens including bacterial pathogens. During our routine survey of commercial onion farms in 2014, 2020, and 2021, seven putative Rouxiella spp. strains were isolated from symptomatic onion samples in Georgia, United States. Upon fulfilling Koch’s postulates on onion, a genome analysis was conducted. Whole-genome indices (ANI and dDDH) showed that the strains belonged to Rouxiella badensis. Although the seven R. badensis strains were not pathogenic on onion foliage, the strains were able to cause bulb rot and could also produce necrotic lesions in a red onion scale assay. R. badensis populations increased significantly and to a level comparable to P. ananatis PNA 97-1R in a red onion scale infection assay. The core-genome analysis grouped all onion R. badensis strains from Georgia together, and distinct from R. badensis strains isolated from other sources and locations. Based on the genome analysis of strains (from the current study and available genomes in the repository), type I, III (Ssa-Esc and Inv-Mxi-Spa types), and V secretion systems are present in R. badensis genomes, while type II, IV, and VI secretion systems are absent. However, various secondary metabolite gene clusters were identified from R. badensis genomes, and a thiol/redox-associated enzyme gene cluster similar to the Pantoea alt cluster mediating thiosulfinate tolerance was also present in onion strains of R. badensis. This is the first report of R. badensis as a plant pathogen. Frontiers Media S.A. 2022-11-30 /pmc/articles/PMC9749400/ /pubmed/36532473 http://dx.doi.org/10.3389/fmicb.2022.1054813 Text en Copyright © 2022 Zhao, Tyson, Gitaitis, Kvitko and Dutta. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Microbiology
Zhao, Mei
Tyson, Chris
Gitaitis, Ron
Kvitko, Brian
Dutta, Bhabesh
Rouxiella badensis, a new bacterial pathogen of onion causing bulb rot
title Rouxiella badensis, a new bacterial pathogen of onion causing bulb rot
title_full Rouxiella badensis, a new bacterial pathogen of onion causing bulb rot
title_fullStr Rouxiella badensis, a new bacterial pathogen of onion causing bulb rot
title_full_unstemmed Rouxiella badensis, a new bacterial pathogen of onion causing bulb rot
title_short Rouxiella badensis, a new bacterial pathogen of onion causing bulb rot
title_sort rouxiella badensis, a new bacterial pathogen of onion causing bulb rot
topic Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9749400/
https://www.ncbi.nlm.nih.gov/pubmed/36532473
http://dx.doi.org/10.3389/fmicb.2022.1054813
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