Cargando…
Rouxiella badensis, a new bacterial pathogen of onion causing bulb rot
Onion bulb rot can be caused by multiple plant pathogens including bacterial pathogens. During our routine survey of commercial onion farms in 2014, 2020, and 2021, seven putative Rouxiella spp. strains were isolated from symptomatic onion samples in Georgia, United States. Upon fulfilling Koch’s po...
Autores principales: | , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Frontiers Media S.A.
2022
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9749400/ https://www.ncbi.nlm.nih.gov/pubmed/36532473 http://dx.doi.org/10.3389/fmicb.2022.1054813 |
_version_ | 1784850034780536832 |
---|---|
author | Zhao, Mei Tyson, Chris Gitaitis, Ron Kvitko, Brian Dutta, Bhabesh |
author_facet | Zhao, Mei Tyson, Chris Gitaitis, Ron Kvitko, Brian Dutta, Bhabesh |
author_sort | Zhao, Mei |
collection | PubMed |
description | Onion bulb rot can be caused by multiple plant pathogens including bacterial pathogens. During our routine survey of commercial onion farms in 2014, 2020, and 2021, seven putative Rouxiella spp. strains were isolated from symptomatic onion samples in Georgia, United States. Upon fulfilling Koch’s postulates on onion, a genome analysis was conducted. Whole-genome indices (ANI and dDDH) showed that the strains belonged to Rouxiella badensis. Although the seven R. badensis strains were not pathogenic on onion foliage, the strains were able to cause bulb rot and could also produce necrotic lesions in a red onion scale assay. R. badensis populations increased significantly and to a level comparable to P. ananatis PNA 97-1R in a red onion scale infection assay. The core-genome analysis grouped all onion R. badensis strains from Georgia together, and distinct from R. badensis strains isolated from other sources and locations. Based on the genome analysis of strains (from the current study and available genomes in the repository), type I, III (Ssa-Esc and Inv-Mxi-Spa types), and V secretion systems are present in R. badensis genomes, while type II, IV, and VI secretion systems are absent. However, various secondary metabolite gene clusters were identified from R. badensis genomes, and a thiol/redox-associated enzyme gene cluster similar to the Pantoea alt cluster mediating thiosulfinate tolerance was also present in onion strains of R. badensis. This is the first report of R. badensis as a plant pathogen. |
format | Online Article Text |
id | pubmed-9749400 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-97494002022-12-15 Rouxiella badensis, a new bacterial pathogen of onion causing bulb rot Zhao, Mei Tyson, Chris Gitaitis, Ron Kvitko, Brian Dutta, Bhabesh Front Microbiol Microbiology Onion bulb rot can be caused by multiple plant pathogens including bacterial pathogens. During our routine survey of commercial onion farms in 2014, 2020, and 2021, seven putative Rouxiella spp. strains were isolated from symptomatic onion samples in Georgia, United States. Upon fulfilling Koch’s postulates on onion, a genome analysis was conducted. Whole-genome indices (ANI and dDDH) showed that the strains belonged to Rouxiella badensis. Although the seven R. badensis strains were not pathogenic on onion foliage, the strains were able to cause bulb rot and could also produce necrotic lesions in a red onion scale assay. R. badensis populations increased significantly and to a level comparable to P. ananatis PNA 97-1R in a red onion scale infection assay. The core-genome analysis grouped all onion R. badensis strains from Georgia together, and distinct from R. badensis strains isolated from other sources and locations. Based on the genome analysis of strains (from the current study and available genomes in the repository), type I, III (Ssa-Esc and Inv-Mxi-Spa types), and V secretion systems are present in R. badensis genomes, while type II, IV, and VI secretion systems are absent. However, various secondary metabolite gene clusters were identified from R. badensis genomes, and a thiol/redox-associated enzyme gene cluster similar to the Pantoea alt cluster mediating thiosulfinate tolerance was also present in onion strains of R. badensis. This is the first report of R. badensis as a plant pathogen. Frontiers Media S.A. 2022-11-30 /pmc/articles/PMC9749400/ /pubmed/36532473 http://dx.doi.org/10.3389/fmicb.2022.1054813 Text en Copyright © 2022 Zhao, Tyson, Gitaitis, Kvitko and Dutta. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Microbiology Zhao, Mei Tyson, Chris Gitaitis, Ron Kvitko, Brian Dutta, Bhabesh Rouxiella badensis, a new bacterial pathogen of onion causing bulb rot |
title | Rouxiella badensis, a new bacterial pathogen of onion causing bulb rot |
title_full | Rouxiella badensis, a new bacterial pathogen of onion causing bulb rot |
title_fullStr | Rouxiella badensis, a new bacterial pathogen of onion causing bulb rot |
title_full_unstemmed | Rouxiella badensis, a new bacterial pathogen of onion causing bulb rot |
title_short | Rouxiella badensis, a new bacterial pathogen of onion causing bulb rot |
title_sort | rouxiella badensis, a new bacterial pathogen of onion causing bulb rot |
topic | Microbiology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9749400/ https://www.ncbi.nlm.nih.gov/pubmed/36532473 http://dx.doi.org/10.3389/fmicb.2022.1054813 |
work_keys_str_mv | AT zhaomei rouxiellabadensisanewbacterialpathogenofonioncausingbulbrot AT tysonchris rouxiellabadensisanewbacterialpathogenofonioncausingbulbrot AT gitaitisron rouxiellabadensisanewbacterialpathogenofonioncausingbulbrot AT kvitkobrian rouxiellabadensisanewbacterialpathogenofonioncausingbulbrot AT duttabhabesh rouxiellabadensisanewbacterialpathogenofonioncausingbulbrot |