Cargando…

Development of a 3D simulator for training the mouse in utero electroporation

In utero electroporation (IUE) requires high-level training in microinjection through the mouse uterine wall into the lateral ventricle of the mouse brain. Training for IUE is currently being performed in live mice as no artificial models allow simulations yet. This study aimed to develop an anatomi...

Descripción completa

Detalles Bibliográficos
Autores principales: Nuber, Maximilian, Gonzalez-Uarquin, Fernando, Neufurth, Meik, Brockmann, Marc A., Baumgart, Jan, Baumgart, Nadine
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9749995/
https://www.ncbi.nlm.nih.gov/pubmed/36516187
http://dx.doi.org/10.1371/journal.pone.0279004
_version_ 1784850151737655296
author Nuber, Maximilian
Gonzalez-Uarquin, Fernando
Neufurth, Meik
Brockmann, Marc A.
Baumgart, Jan
Baumgart, Nadine
author_facet Nuber, Maximilian
Gonzalez-Uarquin, Fernando
Neufurth, Meik
Brockmann, Marc A.
Baumgart, Jan
Baumgart, Nadine
author_sort Nuber, Maximilian
collection PubMed
description In utero electroporation (IUE) requires high-level training in microinjection through the mouse uterine wall into the lateral ventricle of the mouse brain. Training for IUE is currently being performed in live mice as no artificial models allow simulations yet. This study aimed to develop an anatomically realistic 3D printed simulator to train IUE in mice. To this end, we created embryo models containing lateral ventricles. We coupled them to uterus models in six steps: (1) computed tomography imaging, (2) 3D model segmentation, (3) 3D model refinement, (4) mold creation to cast the actual model, (5) 3D mold printing, and (6) mold casting the molds with a mix of soft silicones to ensure the hardness and consistency of the uterus and embryo. The results showed that the simulator assembly successfully recreated the IUE. The compression test did not differ in the mechanical properties of the real embryo or in the required load for uterus displacement. Furthermore, more than 90% of the users approved the simulator as an introduction to IUE and considered that the simulator could help reduce the number of animals for training. Despite current limitations, our 3D simulator enabled a realistic experience for initial approximations to the IUE and is a real alternative for implementing the 3Rs. We are currently working on refining the model.
format Online
Article
Text
id pubmed-9749995
institution National Center for Biotechnology Information
language English
publishDate 2022
publisher Public Library of Science
record_format MEDLINE/PubMed
spelling pubmed-97499952022-12-15 Development of a 3D simulator for training the mouse in utero electroporation Nuber, Maximilian Gonzalez-Uarquin, Fernando Neufurth, Meik Brockmann, Marc A. Baumgart, Jan Baumgart, Nadine PLoS One Research Article In utero electroporation (IUE) requires high-level training in microinjection through the mouse uterine wall into the lateral ventricle of the mouse brain. Training for IUE is currently being performed in live mice as no artificial models allow simulations yet. This study aimed to develop an anatomically realistic 3D printed simulator to train IUE in mice. To this end, we created embryo models containing lateral ventricles. We coupled them to uterus models in six steps: (1) computed tomography imaging, (2) 3D model segmentation, (3) 3D model refinement, (4) mold creation to cast the actual model, (5) 3D mold printing, and (6) mold casting the molds with a mix of soft silicones to ensure the hardness and consistency of the uterus and embryo. The results showed that the simulator assembly successfully recreated the IUE. The compression test did not differ in the mechanical properties of the real embryo or in the required load for uterus displacement. Furthermore, more than 90% of the users approved the simulator as an introduction to IUE and considered that the simulator could help reduce the number of animals for training. Despite current limitations, our 3D simulator enabled a realistic experience for initial approximations to the IUE and is a real alternative for implementing the 3Rs. We are currently working on refining the model. Public Library of Science 2022-12-14 /pmc/articles/PMC9749995/ /pubmed/36516187 http://dx.doi.org/10.1371/journal.pone.0279004 Text en © 2022 Nuber et al https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Nuber, Maximilian
Gonzalez-Uarquin, Fernando
Neufurth, Meik
Brockmann, Marc A.
Baumgart, Jan
Baumgart, Nadine
Development of a 3D simulator for training the mouse in utero electroporation
title Development of a 3D simulator for training the mouse in utero electroporation
title_full Development of a 3D simulator for training the mouse in utero electroporation
title_fullStr Development of a 3D simulator for training the mouse in utero electroporation
title_full_unstemmed Development of a 3D simulator for training the mouse in utero electroporation
title_short Development of a 3D simulator for training the mouse in utero electroporation
title_sort development of a 3d simulator for training the mouse in utero electroporation
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9749995/
https://www.ncbi.nlm.nih.gov/pubmed/36516187
http://dx.doi.org/10.1371/journal.pone.0279004
work_keys_str_mv AT nubermaximilian developmentofa3dsimulatorfortrainingthemouseinuteroelectroporation
AT gonzalezuarquinfernando developmentofa3dsimulatorfortrainingthemouseinuteroelectroporation
AT neufurthmeik developmentofa3dsimulatorfortrainingthemouseinuteroelectroporation
AT brockmannmarca developmentofa3dsimulatorfortrainingthemouseinuteroelectroporation
AT baumgartjan developmentofa3dsimulatorfortrainingthemouseinuteroelectroporation
AT baumgartnadine developmentofa3dsimulatorfortrainingthemouseinuteroelectroporation