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Plasma proteomics reveals early, broad release of chemokine, cytokine, TNF, and interferon mediators following trauma with delayed increases in a subset of chemokines and cytokines in patients that remain critically ill

Severe injury is known to cause a systemic cytokine storm that is associated with adverse outcomes. However, a comprehensive assessment of the time-dependent changes in circulating levels of a broad spectrum of protein immune mediators and soluble immune mediator receptors in severely injured trauma...

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Autores principales: Bonaroti, Jillian, Billiar, Isabel, Moheimani, Hamed, Wu, Junru, Namas, Rami, Li, Shimena, Kar, Upendra K., Vodovotz, Yoram, Neal, Matthew D., Sperry, Jason L., Billiar, Timothy R.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9750757/
https://www.ncbi.nlm.nih.gov/pubmed/36532045
http://dx.doi.org/10.3389/fimmu.2022.1038086
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author Bonaroti, Jillian
Billiar, Isabel
Moheimani, Hamed
Wu, Junru
Namas, Rami
Li, Shimena
Kar, Upendra K.
Vodovotz, Yoram
Neal, Matthew D.
Sperry, Jason L.
Billiar, Timothy R.
author_facet Bonaroti, Jillian
Billiar, Isabel
Moheimani, Hamed
Wu, Junru
Namas, Rami
Li, Shimena
Kar, Upendra K.
Vodovotz, Yoram
Neal, Matthew D.
Sperry, Jason L.
Billiar, Timothy R.
author_sort Bonaroti, Jillian
collection PubMed
description Severe injury is known to cause a systemic cytokine storm that is associated with adverse outcomes. However, a comprehensive assessment of the time-dependent changes in circulating levels of a broad spectrum of protein immune mediators and soluble immune mediator receptors in severely injured trauma patients remains uncharacterized. To address this knowledge gap, we defined the temporal and outcome-based patterns of 184 known immune mediators and soluble cytokine receptors in the circulation of severely injured patients. Proteomics (aptamer-based assay, SomaLogic, Inc) was performed on plasma samples drawn at 0, 24, and 72 hours (h) from time of admission from 150 trauma patients, a representative subset from the Prehospital Plasma during Air Medical Transport in Trauma Patients at Risk for Hemorrhagic Shock (PAMPer) trial. Patients were categorized into outcome groups including Early Non-Survivors (died within 72 h; ENS; n=38), Non-Resolvers (died after 72 h or required ≥7 days of intensive care; NR; n=78), and Resolvers (survivors that required < 7 days of intensive care; R; n=34), with low Injury Severity Score (ISS) patients from the Tranexamic Acid During Prehospital Transport in Patients at Risk for Hemorrhage After Injury (STAAMP) trial as controls. The major findings include an extensive release of immune mediators and cytokine receptors at time 0h that is more pronounced in ENS and NR patients. There was a selective subset of mediators elevated at 24 and 72 h to a greater degree in NR patients, including multiple cytokines and chemokines not previously described in trauma patients. These findings were validated in a quantitative fashion using mesoscale discovery immunoassays (MSD) from an external validation cohort (VC) of samples from 58 trauma patients matched for R and NR status. This comprehensive longitudinal description of immune mediator patterns associated with trauma outcomes provides a new level of characterization of the immune response that follows severe injury.
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spelling pubmed-97507572022-12-15 Plasma proteomics reveals early, broad release of chemokine, cytokine, TNF, and interferon mediators following trauma with delayed increases in a subset of chemokines and cytokines in patients that remain critically ill Bonaroti, Jillian Billiar, Isabel Moheimani, Hamed Wu, Junru Namas, Rami Li, Shimena Kar, Upendra K. Vodovotz, Yoram Neal, Matthew D. Sperry, Jason L. Billiar, Timothy R. Front Immunol Immunology Severe injury is known to cause a systemic cytokine storm that is associated with adverse outcomes. However, a comprehensive assessment of the time-dependent changes in circulating levels of a broad spectrum of protein immune mediators and soluble immune mediator receptors in severely injured trauma patients remains uncharacterized. To address this knowledge gap, we defined the temporal and outcome-based patterns of 184 known immune mediators and soluble cytokine receptors in the circulation of severely injured patients. Proteomics (aptamer-based assay, SomaLogic, Inc) was performed on plasma samples drawn at 0, 24, and 72 hours (h) from time of admission from 150 trauma patients, a representative subset from the Prehospital Plasma during Air Medical Transport in Trauma Patients at Risk for Hemorrhagic Shock (PAMPer) trial. Patients were categorized into outcome groups including Early Non-Survivors (died within 72 h; ENS; n=38), Non-Resolvers (died after 72 h or required ≥7 days of intensive care; NR; n=78), and Resolvers (survivors that required < 7 days of intensive care; R; n=34), with low Injury Severity Score (ISS) patients from the Tranexamic Acid During Prehospital Transport in Patients at Risk for Hemorrhage After Injury (STAAMP) trial as controls. The major findings include an extensive release of immune mediators and cytokine receptors at time 0h that is more pronounced in ENS and NR patients. There was a selective subset of mediators elevated at 24 and 72 h to a greater degree in NR patients, including multiple cytokines and chemokines not previously described in trauma patients. These findings were validated in a quantitative fashion using mesoscale discovery immunoassays (MSD) from an external validation cohort (VC) of samples from 58 trauma patients matched for R and NR status. This comprehensive longitudinal description of immune mediator patterns associated with trauma outcomes provides a new level of characterization of the immune response that follows severe injury. Frontiers Media S.A. 2022-11-30 /pmc/articles/PMC9750757/ /pubmed/36532045 http://dx.doi.org/10.3389/fimmu.2022.1038086 Text en Copyright © 2022 Bonaroti, Billiar, Moheimani, Wu, Namas, Li, Kar, Vodovotz, Neal, Sperry and Billiar https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Immunology
Bonaroti, Jillian
Billiar, Isabel
Moheimani, Hamed
Wu, Junru
Namas, Rami
Li, Shimena
Kar, Upendra K.
Vodovotz, Yoram
Neal, Matthew D.
Sperry, Jason L.
Billiar, Timothy R.
Plasma proteomics reveals early, broad release of chemokine, cytokine, TNF, and interferon mediators following trauma with delayed increases in a subset of chemokines and cytokines in patients that remain critically ill
title Plasma proteomics reveals early, broad release of chemokine, cytokine, TNF, and interferon mediators following trauma with delayed increases in a subset of chemokines and cytokines in patients that remain critically ill
title_full Plasma proteomics reveals early, broad release of chemokine, cytokine, TNF, and interferon mediators following trauma with delayed increases in a subset of chemokines and cytokines in patients that remain critically ill
title_fullStr Plasma proteomics reveals early, broad release of chemokine, cytokine, TNF, and interferon mediators following trauma with delayed increases in a subset of chemokines and cytokines in patients that remain critically ill
title_full_unstemmed Plasma proteomics reveals early, broad release of chemokine, cytokine, TNF, and interferon mediators following trauma with delayed increases in a subset of chemokines and cytokines in patients that remain critically ill
title_short Plasma proteomics reveals early, broad release of chemokine, cytokine, TNF, and interferon mediators following trauma with delayed increases in a subset of chemokines and cytokines in patients that remain critically ill
title_sort plasma proteomics reveals early, broad release of chemokine, cytokine, tnf, and interferon mediators following trauma with delayed increases in a subset of chemokines and cytokines in patients that remain critically ill
topic Immunology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9750757/
https://www.ncbi.nlm.nih.gov/pubmed/36532045
http://dx.doi.org/10.3389/fimmu.2022.1038086
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