Cargando…

Wound healing approach based on excretory-secretory product and lysate of liver flukes

Exogenous bioactive peptides are considered promising for the wound healing therapy in humans. In this regard, parasitic trematodes proteins may potentially become a new perspective agents. Foodborne trematode Opisthorchis felineus is widespread in Europe and has the ability to stimulate proliferati...

Descripción completa

Detalles Bibliográficos
Autores principales: Kovner, Anna V., Tarasenko, Alena A., Zaparina, Oxana, Tikhonova, Olga V., Pakharukova, Maria Y., Mordvinov, Viatcheslav A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9751068/
https://www.ncbi.nlm.nih.gov/pubmed/36517588
http://dx.doi.org/10.1038/s41598-022-26275-y
_version_ 1784850393591709696
author Kovner, Anna V.
Tarasenko, Alena A.
Zaparina, Oxana
Tikhonova, Olga V.
Pakharukova, Maria Y.
Mordvinov, Viatcheslav A.
author_facet Kovner, Anna V.
Tarasenko, Alena A.
Zaparina, Oxana
Tikhonova, Olga V.
Pakharukova, Maria Y.
Mordvinov, Viatcheslav A.
author_sort Kovner, Anna V.
collection PubMed
description Exogenous bioactive peptides are considered promising for the wound healing therapy in humans. In this regard, parasitic trematodes proteins may potentially become a new perspective agents. Foodborne trematode Opisthorchis felineus is widespread in Europe and has the ability to stimulate proliferation of bile duct epithelium. In this study, we investigated skin wound healing potential of O. felineus proteins in mouse model. C57Bl/6 mice were inflicted with superficial wounds with 8 mm diameter. Experimental groups included several non-specific controls and specific treatment groups (excretory-secretory product and lysate). After 10 days of the experiment, the percentage of wound healing in the specific treatment groups significantly exceeded the control values. We also found that wound treatment with excretory-secretory product and worm lysate resulted in: (i) inflammation reducing, (ii) vascular response modulating, (iii) type 1 collagen deposition promoting dermal ECM remodeling. An additional proteomic analysis of excretory-secretory product and worm lysate samples was revealed 111 common proteins. The obtained data indicate a high wound-healing potential of liver fluke proteins and open prospects for further research as new therapeutic approaches.
format Online
Article
Text
id pubmed-9751068
institution National Center for Biotechnology Information
language English
publishDate 2022
publisher Nature Publishing Group UK
record_format MEDLINE/PubMed
spelling pubmed-97510682022-12-16 Wound healing approach based on excretory-secretory product and lysate of liver flukes Kovner, Anna V. Tarasenko, Alena A. Zaparina, Oxana Tikhonova, Olga V. Pakharukova, Maria Y. Mordvinov, Viatcheslav A. Sci Rep Article Exogenous bioactive peptides are considered promising for the wound healing therapy in humans. In this regard, parasitic trematodes proteins may potentially become a new perspective agents. Foodborne trematode Opisthorchis felineus is widespread in Europe and has the ability to stimulate proliferation of bile duct epithelium. In this study, we investigated skin wound healing potential of O. felineus proteins in mouse model. C57Bl/6 mice were inflicted with superficial wounds with 8 mm diameter. Experimental groups included several non-specific controls and specific treatment groups (excretory-secretory product and lysate). After 10 days of the experiment, the percentage of wound healing in the specific treatment groups significantly exceeded the control values. We also found that wound treatment with excretory-secretory product and worm lysate resulted in: (i) inflammation reducing, (ii) vascular response modulating, (iii) type 1 collagen deposition promoting dermal ECM remodeling. An additional proteomic analysis of excretory-secretory product and worm lysate samples was revealed 111 common proteins. The obtained data indicate a high wound-healing potential of liver fluke proteins and open prospects for further research as new therapeutic approaches. Nature Publishing Group UK 2022-12-14 /pmc/articles/PMC9751068/ /pubmed/36517588 http://dx.doi.org/10.1038/s41598-022-26275-y Text en © The Author(s) 2022 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Article
Kovner, Anna V.
Tarasenko, Alena A.
Zaparina, Oxana
Tikhonova, Olga V.
Pakharukova, Maria Y.
Mordvinov, Viatcheslav A.
Wound healing approach based on excretory-secretory product and lysate of liver flukes
title Wound healing approach based on excretory-secretory product and lysate of liver flukes
title_full Wound healing approach based on excretory-secretory product and lysate of liver flukes
title_fullStr Wound healing approach based on excretory-secretory product and lysate of liver flukes
title_full_unstemmed Wound healing approach based on excretory-secretory product and lysate of liver flukes
title_short Wound healing approach based on excretory-secretory product and lysate of liver flukes
title_sort wound healing approach based on excretory-secretory product and lysate of liver flukes
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9751068/
https://www.ncbi.nlm.nih.gov/pubmed/36517588
http://dx.doi.org/10.1038/s41598-022-26275-y
work_keys_str_mv AT kovnerannav woundhealingapproachbasedonexcretorysecretoryproductandlysateofliverflukes
AT tarasenkoalenaa woundhealingapproachbasedonexcretorysecretoryproductandlysateofliverflukes
AT zaparinaoxana woundhealingapproachbasedonexcretorysecretoryproductandlysateofliverflukes
AT tikhonovaolgav woundhealingapproachbasedonexcretorysecretoryproductandlysateofliverflukes
AT pakharukovamariay woundhealingapproachbasedonexcretorysecretoryproductandlysateofliverflukes
AT mordvinovviatcheslava woundhealingapproachbasedonexcretorysecretoryproductandlysateofliverflukes