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Gold Nanoparticles Enhancing Generation of ROS for Cs-137 Radiotherapy

Radiotherapy is an important modality for the treatment of cancer, e.g., X-ray, Cs-137 γ-ray (peak energy: 662 keV). An important therapy pathway of radiation is to generate the double strand breaks of DNA to prohibit the proliferation of cancer cells. In addition, the excessive amount of reactive o...

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Autores principales: Tsai, Shiao-Wen, Lo, Chang-Yun, Yu, Shang-Yang, Chen, Fang-Hsin, Huang, Hsiao-Chieh, Wang, Lu-Kai, Liaw, Jiunn-Woei
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer US 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9751242/
https://www.ncbi.nlm.nih.gov/pubmed/36515781
http://dx.doi.org/10.1186/s11671-022-03761-w
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author Tsai, Shiao-Wen
Lo, Chang-Yun
Yu, Shang-Yang
Chen, Fang-Hsin
Huang, Hsiao-Chieh
Wang, Lu-Kai
Liaw, Jiunn-Woei
author_facet Tsai, Shiao-Wen
Lo, Chang-Yun
Yu, Shang-Yang
Chen, Fang-Hsin
Huang, Hsiao-Chieh
Wang, Lu-Kai
Liaw, Jiunn-Woei
author_sort Tsai, Shiao-Wen
collection PubMed
description Radiotherapy is an important modality for the treatment of cancer, e.g., X-ray, Cs-137 γ-ray (peak energy: 662 keV). An important therapy pathway of radiation is to generate the double strand breaks of DNA to prohibit the proliferation of cancer cells. In addition, the excessive amount of reactive oxygen species (ROS) is induced to damage the organelles, which can cause cellular apoptosis or necrosis. Gold nanoparticles (GNPs) have been proven potential as a radiosensitizer due to the high biocompatibility, the low cytotoxicity and the high-Z property (Z = 79) of gold. The latter property may allow GNPs to induce more secondary electrons for generating ROS in cells as irradiated by high-energy photons. In this paper, the radiobiological effects on A431 cells with uptake of 55-nm GNPs were studied to investigate the GNPs-enhanced production of ROS on these cells as irradiated by Cs-137 γ-ray. The fluorescence-labeling image of laser scanning confocal microscopy (LSCM) shows the excessive expression of ROS in these GNPs-uptake cells after irradiation. And then, the follow-up disruption of cytoskeletons and dysfunction of mitochondria caused by the induced ROS are observed. From the curves of cell survival fraction versus the radiation dose, the radiosensitization enhancement factor of GNPs is 1.29 at a survival fraction of 30%. This demonstrates that the tumoricidal efficacy of Cs-137 radiation can be significantly raised by GNPs. Because of facilitating the production of excessive ROS to damage tumor cells, GNPs are proven to be a prospective radiosensitizer for radiotherapy, particularly for the treatment of certain radioresistant tumor cells. Through this pathway, the tumoricidal efficacy of radiotherapy can be raised. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s11671-022-03761-w.
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spelling pubmed-97512422022-12-16 Gold Nanoparticles Enhancing Generation of ROS for Cs-137 Radiotherapy Tsai, Shiao-Wen Lo, Chang-Yun Yu, Shang-Yang Chen, Fang-Hsin Huang, Hsiao-Chieh Wang, Lu-Kai Liaw, Jiunn-Woei Nanoscale Res Lett Research Radiotherapy is an important modality for the treatment of cancer, e.g., X-ray, Cs-137 γ-ray (peak energy: 662 keV). An important therapy pathway of radiation is to generate the double strand breaks of DNA to prohibit the proliferation of cancer cells. In addition, the excessive amount of reactive oxygen species (ROS) is induced to damage the organelles, which can cause cellular apoptosis or necrosis. Gold nanoparticles (GNPs) have been proven potential as a radiosensitizer due to the high biocompatibility, the low cytotoxicity and the high-Z property (Z = 79) of gold. The latter property may allow GNPs to induce more secondary electrons for generating ROS in cells as irradiated by high-energy photons. In this paper, the radiobiological effects on A431 cells with uptake of 55-nm GNPs were studied to investigate the GNPs-enhanced production of ROS on these cells as irradiated by Cs-137 γ-ray. The fluorescence-labeling image of laser scanning confocal microscopy (LSCM) shows the excessive expression of ROS in these GNPs-uptake cells after irradiation. And then, the follow-up disruption of cytoskeletons and dysfunction of mitochondria caused by the induced ROS are observed. From the curves of cell survival fraction versus the radiation dose, the radiosensitization enhancement factor of GNPs is 1.29 at a survival fraction of 30%. This demonstrates that the tumoricidal efficacy of Cs-137 radiation can be significantly raised by GNPs. Because of facilitating the production of excessive ROS to damage tumor cells, GNPs are proven to be a prospective radiosensitizer for radiotherapy, particularly for the treatment of certain radioresistant tumor cells. Through this pathway, the tumoricidal efficacy of radiotherapy can be raised. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s11671-022-03761-w. Springer US 2022-12-14 /pmc/articles/PMC9751242/ /pubmed/36515781 http://dx.doi.org/10.1186/s11671-022-03761-w Text en © The Author(s) 2022 https://creativecommons.org/licenses/by/4.0/Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Research
Tsai, Shiao-Wen
Lo, Chang-Yun
Yu, Shang-Yang
Chen, Fang-Hsin
Huang, Hsiao-Chieh
Wang, Lu-Kai
Liaw, Jiunn-Woei
Gold Nanoparticles Enhancing Generation of ROS for Cs-137 Radiotherapy
title Gold Nanoparticles Enhancing Generation of ROS for Cs-137 Radiotherapy
title_full Gold Nanoparticles Enhancing Generation of ROS for Cs-137 Radiotherapy
title_fullStr Gold Nanoparticles Enhancing Generation of ROS for Cs-137 Radiotherapy
title_full_unstemmed Gold Nanoparticles Enhancing Generation of ROS for Cs-137 Radiotherapy
title_short Gold Nanoparticles Enhancing Generation of ROS for Cs-137 Radiotherapy
title_sort gold nanoparticles enhancing generation of ros for cs-137 radiotherapy
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9751242/
https://www.ncbi.nlm.nih.gov/pubmed/36515781
http://dx.doi.org/10.1186/s11671-022-03761-w
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