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Characterization and expression profiling of PIN auxin efflux transporters reveal their role in developmental and abiotic stress conditions in rice

The auxin efflux transporter proteins called PINs ferry auxin from its source to sinks in particular directions depending on their polar localizations in the plasma membrane, thus facilitating the development of the entire plant architecture. The rice genome has 12 PIN genes distributed over eight c...

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Autores principales: Manna, Mrinalini, Rengasamy, Balakrishnan, Ambasht, Navin Kumar, Sinha, Alok Krishna
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9751476/
https://www.ncbi.nlm.nih.gov/pubmed/36531415
http://dx.doi.org/10.3389/fpls.2022.1059559
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author Manna, Mrinalini
Rengasamy, Balakrishnan
Ambasht, Navin Kumar
Sinha, Alok Krishna
author_facet Manna, Mrinalini
Rengasamy, Balakrishnan
Ambasht, Navin Kumar
Sinha, Alok Krishna
author_sort Manna, Mrinalini
collection PubMed
description The auxin efflux transporter proteins called PINs ferry auxin from its source to sinks in particular directions depending on their polar localizations in the plasma membrane, thus facilitating the development of the entire plant architecture. The rice genome has 12 PIN genes distributed over eight chromosomes. To study their roles in plant development, abiotic stress responsiveness, and shaping an auxin-dependent root architecture, a genome-wide analysis was carried out. Based on phylogeny, cellular localization, and hydrophilic loop domain size, the PINs were categorized into canonical and noncanonical PINs. PINs were found expressed in all of the organs of plants that emphasized their indispensable role throughout the plant’s life cycle. We discovered that PIN5C and PIN9 were upregulated during salt and drought stress. We also found that regardless of its cellular level, auxin functioned as a molecular switch to turn on auxin biosynthesis genes. On the contrary, although PIN expression was upregulated upon initial treatment with auxin, prolonged auxin treatment not only led to their downregulation but also led to the development of auxin-dependent altered root formation in rice. Our study paves the way for developing stress-tolerant rice and plants with a desirable root architecture by genetic engineering.
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spelling pubmed-97514762022-12-16 Characterization and expression profiling of PIN auxin efflux transporters reveal their role in developmental and abiotic stress conditions in rice Manna, Mrinalini Rengasamy, Balakrishnan Ambasht, Navin Kumar Sinha, Alok Krishna Front Plant Sci Plant Science The auxin efflux transporter proteins called PINs ferry auxin from its source to sinks in particular directions depending on their polar localizations in the plasma membrane, thus facilitating the development of the entire plant architecture. The rice genome has 12 PIN genes distributed over eight chromosomes. To study their roles in plant development, abiotic stress responsiveness, and shaping an auxin-dependent root architecture, a genome-wide analysis was carried out. Based on phylogeny, cellular localization, and hydrophilic loop domain size, the PINs were categorized into canonical and noncanonical PINs. PINs were found expressed in all of the organs of plants that emphasized their indispensable role throughout the plant’s life cycle. We discovered that PIN5C and PIN9 were upregulated during salt and drought stress. We also found that regardless of its cellular level, auxin functioned as a molecular switch to turn on auxin biosynthesis genes. On the contrary, although PIN expression was upregulated upon initial treatment with auxin, prolonged auxin treatment not only led to their downregulation but also led to the development of auxin-dependent altered root formation in rice. Our study paves the way for developing stress-tolerant rice and plants with a desirable root architecture by genetic engineering. Frontiers Media S.A. 2022-12-01 /pmc/articles/PMC9751476/ /pubmed/36531415 http://dx.doi.org/10.3389/fpls.2022.1059559 Text en Copyright © 2022 Manna, Rengasamy, Ambasht and Sinha https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Plant Science
Manna, Mrinalini
Rengasamy, Balakrishnan
Ambasht, Navin Kumar
Sinha, Alok Krishna
Characterization and expression profiling of PIN auxin efflux transporters reveal their role in developmental and abiotic stress conditions in rice
title Characterization and expression profiling of PIN auxin efflux transporters reveal their role in developmental and abiotic stress conditions in rice
title_full Characterization and expression profiling of PIN auxin efflux transporters reveal their role in developmental and abiotic stress conditions in rice
title_fullStr Characterization and expression profiling of PIN auxin efflux transporters reveal their role in developmental and abiotic stress conditions in rice
title_full_unstemmed Characterization and expression profiling of PIN auxin efflux transporters reveal their role in developmental and abiotic stress conditions in rice
title_short Characterization and expression profiling of PIN auxin efflux transporters reveal their role in developmental and abiotic stress conditions in rice
title_sort characterization and expression profiling of pin auxin efflux transporters reveal their role in developmental and abiotic stress conditions in rice
topic Plant Science
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9751476/
https://www.ncbi.nlm.nih.gov/pubmed/36531415
http://dx.doi.org/10.3389/fpls.2022.1059559
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