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Sequence-specific capture and concentration of viral RNA by type III CRISPR system enhances diagnostic
Type-III CRISPR-Cas systems have recently been adopted for sequence-specific detection of SARS-CoV-2. Here, we repurpose the type III-A CRISPR complex from Thermus thermophilus (TtCsm) for programmable capture and concentration of specific RNAs from complex mixtures. The target bound TtCsm complex g...
Autores principales: | Nemudraia, Anna, Nemudryi, Artem, Buyukyoruk, Murat, Scherffius, Andrew M., Zahl, Trevor, Wiegand, Tanner, Pandey, Shishir, Nichols, Joseph E., Hall, Laina N., McVey, Aidan, Lee, Helen H., Wilkinson, Royce A., Snyder, Laura R., Jones, Joshua D., Koutmou, Kristin S., Santiago-Frangos, Andrew, Wiedenheft, Blake |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9751510/ https://www.ncbi.nlm.nih.gov/pubmed/36522348 http://dx.doi.org/10.1038/s41467-022-35445-5 |
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