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GhCDPK60 positively regulates drought stress tolerance in both transgenic Arabidopsis and cotton by regulating proline content and ROS level

Calcium-Dependent Protein Kinases (CDPKs) involved in regulating downstream components of calcium signaling pathways play a role in tolerance to abiotic stresses and seed development in plants. However, functions of only a few cotton CDPKs have been clarified at present. In this study, 80 conserved...

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Autores principales: Yan, Mengyuan, Yu, Xiaotian, Zhou, Gen, Sun, Dongli, Hu, Yu, Huang, Chenjue, Zheng, Qintao, Sun, Nan, Wu, Jiayan, Fu, Zhaobin, Li, Libei, Feng, Zhen, Yu, Shuxun
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9751749/
https://www.ncbi.nlm.nih.gov/pubmed/36531339
http://dx.doi.org/10.3389/fpls.2022.1072584
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author Yan, Mengyuan
Yu, Xiaotian
Zhou, Gen
Sun, Dongli
Hu, Yu
Huang, Chenjue
Zheng, Qintao
Sun, Nan
Wu, Jiayan
Fu, Zhaobin
Li, Libei
Feng, Zhen
Yu, Shuxun
author_facet Yan, Mengyuan
Yu, Xiaotian
Zhou, Gen
Sun, Dongli
Hu, Yu
Huang, Chenjue
Zheng, Qintao
Sun, Nan
Wu, Jiayan
Fu, Zhaobin
Li, Libei
Feng, Zhen
Yu, Shuxun
author_sort Yan, Mengyuan
collection PubMed
description Calcium-Dependent Protein Kinases (CDPKs) involved in regulating downstream components of calcium signaling pathways play a role in tolerance to abiotic stresses and seed development in plants. However, functions of only a few cotton CDPKs have been clarified at present. In this study, 80 conserved CDPKs in Gossypium hirsutum L. were identified and characterized, which was divided into four subgroups. Among them, the transcript level of GhCDPK60 was significantly upregulated under drought and several hormone treatments. And we found that the expression levels of several stress-inducible genes down-regulated in GhCDPK60-silence cotton and up-regulated in GhCDPK60-overexpressing Arabidopsis. In addition, physiological analyses demonstrated that GhCDPK60 improved drought stress tolerance by improving the osmotic adjustment ability and reducing the accumulation of reactive oxygen species (ROS) in plants. These findings broaden our understanding of the biological roles of GhCDPK60 and mechanisms underlying drought stress tolerance in cotton.
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spelling pubmed-97517492022-12-16 GhCDPK60 positively regulates drought stress tolerance in both transgenic Arabidopsis and cotton by regulating proline content and ROS level Yan, Mengyuan Yu, Xiaotian Zhou, Gen Sun, Dongli Hu, Yu Huang, Chenjue Zheng, Qintao Sun, Nan Wu, Jiayan Fu, Zhaobin Li, Libei Feng, Zhen Yu, Shuxun Front Plant Sci Plant Science Calcium-Dependent Protein Kinases (CDPKs) involved in regulating downstream components of calcium signaling pathways play a role in tolerance to abiotic stresses and seed development in plants. However, functions of only a few cotton CDPKs have been clarified at present. In this study, 80 conserved CDPKs in Gossypium hirsutum L. were identified and characterized, which was divided into four subgroups. Among them, the transcript level of GhCDPK60 was significantly upregulated under drought and several hormone treatments. And we found that the expression levels of several stress-inducible genes down-regulated in GhCDPK60-silence cotton and up-regulated in GhCDPK60-overexpressing Arabidopsis. In addition, physiological analyses demonstrated that GhCDPK60 improved drought stress tolerance by improving the osmotic adjustment ability and reducing the accumulation of reactive oxygen species (ROS) in plants. These findings broaden our understanding of the biological roles of GhCDPK60 and mechanisms underlying drought stress tolerance in cotton. Frontiers Media S.A. 2022-12-01 /pmc/articles/PMC9751749/ /pubmed/36531339 http://dx.doi.org/10.3389/fpls.2022.1072584 Text en Copyright © 2022 Yan, Yu, Zhou, Sun, Hu, Huang, Zheng, Sun, Wu, Fu, Li, Feng and Yu https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Plant Science
Yan, Mengyuan
Yu, Xiaotian
Zhou, Gen
Sun, Dongli
Hu, Yu
Huang, Chenjue
Zheng, Qintao
Sun, Nan
Wu, Jiayan
Fu, Zhaobin
Li, Libei
Feng, Zhen
Yu, Shuxun
GhCDPK60 positively regulates drought stress tolerance in both transgenic Arabidopsis and cotton by regulating proline content and ROS level
title GhCDPK60 positively regulates drought stress tolerance in both transgenic Arabidopsis and cotton by regulating proline content and ROS level
title_full GhCDPK60 positively regulates drought stress tolerance in both transgenic Arabidopsis and cotton by regulating proline content and ROS level
title_fullStr GhCDPK60 positively regulates drought stress tolerance in both transgenic Arabidopsis and cotton by regulating proline content and ROS level
title_full_unstemmed GhCDPK60 positively regulates drought stress tolerance in both transgenic Arabidopsis and cotton by regulating proline content and ROS level
title_short GhCDPK60 positively regulates drought stress tolerance in both transgenic Arabidopsis and cotton by regulating proline content and ROS level
title_sort ghcdpk60 positively regulates drought stress tolerance in both transgenic arabidopsis and cotton by regulating proline content and ros level
topic Plant Science
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9751749/
https://www.ncbi.nlm.nih.gov/pubmed/36531339
http://dx.doi.org/10.3389/fpls.2022.1072584
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