Cargando…

Composition and diversity of soil bacterial communities under identical vegetation along an elevational gradient in Changbai Mountains, China

Soil bacteria play important roles in biogeochemical cycling and biodiversity in mountain ecosystems. Past studies have investigated the bacterial community composition and diversity in elevation gradations covered by different vegetation types, but for a better assessment of elevation effects, here...

Descripción completa

Detalles Bibliográficos
Autores principales: Li, Mengsha, Dai, Guanhua, Mu, Liqiang
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9751831/
https://www.ncbi.nlm.nih.gov/pubmed/36532438
http://dx.doi.org/10.3389/fmicb.2022.1065412
_version_ 1784850569685368832
author Li, Mengsha
Dai, Guanhua
Mu, Liqiang
author_facet Li, Mengsha
Dai, Guanhua
Mu, Liqiang
author_sort Li, Mengsha
collection PubMed
description Soil bacteria play important roles in biogeochemical cycling and biodiversity in mountain ecosystems. Past studies have investigated the bacterial community composition and diversity in elevation gradations covered by different vegetation types, but for a better assessment of elevation effects, here we studied bacterial communities in soil under identical vegetation cover. High-throughput amplicon sequencing of the V3-V4 region of bacterial 16S rDNA was used to investigate the diversity and composition bacterial communities in soil from 700 to 1,000 m above sea level collected on the north slope of Changbai Mountains, Northeast China. Obviously differences (p < 0.05) in soil physicochemical parameters (i.e., total nitrogen, nitrate and ammonium nitrogen, soil moisture content, available potassium, microbial biomass carbon and nitrogen) were observed at different elevations. Soil bacterial abundance indices (Richness, Chao1, ACE) differed significantly along the elevation gradient, whereas the Shannon index remained unchanged. Principal Coordinates Analysis indicated separated soil bacterial communities of the different elevations. The dominant phyla in all soil samples were Proteobacteria, Acidobacteria, Actinobacteria, Verrucomicrobia, and Bacteroidetes, which in combination reached 80%–85%. Soil pH to some extend related to soil bacterial community along altitude gradations. The relative abundance of a multiple phyla was negatively affected by the soil nutrients, such as ammonium and nitrate nitrogen, available potassium, soil moisture content, available phosphorus, microbial biomass nitrogen and soil organic C. The strongest effects were seen for Proteobacteria. The pH either positively or negatively correlated with specific genera. The soil bacterial function differed significantly among four elevations. The chemoheterotrophy, aerobic chemoheterotrophy and nitrification were the most dominant functions of soil bacteria among four elevations. Overall, the changes in soil physicochemical properties with elevation are important in shaping the bacterial diversity, composition and function in soil with the same above-ground vegetation of Changbai Mountains.
format Online
Article
Text
id pubmed-9751831
institution National Center for Biotechnology Information
language English
publishDate 2022
publisher Frontiers Media S.A.
record_format MEDLINE/PubMed
spelling pubmed-97518312022-12-16 Composition and diversity of soil bacterial communities under identical vegetation along an elevational gradient in Changbai Mountains, China Li, Mengsha Dai, Guanhua Mu, Liqiang Front Microbiol Microbiology Soil bacteria play important roles in biogeochemical cycling and biodiversity in mountain ecosystems. Past studies have investigated the bacterial community composition and diversity in elevation gradations covered by different vegetation types, but for a better assessment of elevation effects, here we studied bacterial communities in soil under identical vegetation cover. High-throughput amplicon sequencing of the V3-V4 region of bacterial 16S rDNA was used to investigate the diversity and composition bacterial communities in soil from 700 to 1,000 m above sea level collected on the north slope of Changbai Mountains, Northeast China. Obviously differences (p < 0.05) in soil physicochemical parameters (i.e., total nitrogen, nitrate and ammonium nitrogen, soil moisture content, available potassium, microbial biomass carbon and nitrogen) were observed at different elevations. Soil bacterial abundance indices (Richness, Chao1, ACE) differed significantly along the elevation gradient, whereas the Shannon index remained unchanged. Principal Coordinates Analysis indicated separated soil bacterial communities of the different elevations. The dominant phyla in all soil samples were Proteobacteria, Acidobacteria, Actinobacteria, Verrucomicrobia, and Bacteroidetes, which in combination reached 80%–85%. Soil pH to some extend related to soil bacterial community along altitude gradations. The relative abundance of a multiple phyla was negatively affected by the soil nutrients, such as ammonium and nitrate nitrogen, available potassium, soil moisture content, available phosphorus, microbial biomass nitrogen and soil organic C. The strongest effects were seen for Proteobacteria. The pH either positively or negatively correlated with specific genera. The soil bacterial function differed significantly among four elevations. The chemoheterotrophy, aerobic chemoheterotrophy and nitrification were the most dominant functions of soil bacteria among four elevations. Overall, the changes in soil physicochemical properties with elevation are important in shaping the bacterial diversity, composition and function in soil with the same above-ground vegetation of Changbai Mountains. Frontiers Media S.A. 2022-12-01 /pmc/articles/PMC9751831/ /pubmed/36532438 http://dx.doi.org/10.3389/fmicb.2022.1065412 Text en Copyright © 2022 Li, Dai and Mu. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Microbiology
Li, Mengsha
Dai, Guanhua
Mu, Liqiang
Composition and diversity of soil bacterial communities under identical vegetation along an elevational gradient in Changbai Mountains, China
title Composition and diversity of soil bacterial communities under identical vegetation along an elevational gradient in Changbai Mountains, China
title_full Composition and diversity of soil bacterial communities under identical vegetation along an elevational gradient in Changbai Mountains, China
title_fullStr Composition and diversity of soil bacterial communities under identical vegetation along an elevational gradient in Changbai Mountains, China
title_full_unstemmed Composition and diversity of soil bacterial communities under identical vegetation along an elevational gradient in Changbai Mountains, China
title_short Composition and diversity of soil bacterial communities under identical vegetation along an elevational gradient in Changbai Mountains, China
title_sort composition and diversity of soil bacterial communities under identical vegetation along an elevational gradient in changbai mountains, china
topic Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9751831/
https://www.ncbi.nlm.nih.gov/pubmed/36532438
http://dx.doi.org/10.3389/fmicb.2022.1065412
work_keys_str_mv AT limengsha compositionanddiversityofsoilbacterialcommunitiesunderidenticalvegetationalonganelevationalgradientinchangbaimountainschina
AT daiguanhua compositionanddiversityofsoilbacterialcommunitiesunderidenticalvegetationalonganelevationalgradientinchangbaimountainschina
AT muliqiang compositionanddiversityofsoilbacterialcommunitiesunderidenticalvegetationalonganelevationalgradientinchangbaimountainschina