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Dynamics of symbiotic bacterial community in whole life stage of Harmonia axyridis (Coleoptera: Coccinellidae)

INTRODUCTION: Bacteria play critical roles in the reproduction, metabolism, physiology, and detoxification of their insect hosts. The ladybird beetle (Harmonia axyridis) harbors a myriad of endosymbiotic microbes. However, to date, little is known about how the microbial composition of H. axyridis v...

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Autores principales: Du, Lingen, Xue, Hui, Hu, Fangmei, Zhu, Xiangzhen, Wang, Li, Zhang, Kaixin, Li, Dongyang, Ji, Jichao, Niu, Lin, Luo, Junyu, Cui, Jinjie, Gao, Xueke
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9751998/
https://www.ncbi.nlm.nih.gov/pubmed/36532478
http://dx.doi.org/10.3389/fmicb.2022.1050329
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author Du, Lingen
Xue, Hui
Hu, Fangmei
Zhu, Xiangzhen
Wang, Li
Zhang, Kaixin
Li, Dongyang
Ji, Jichao
Niu, Lin
Luo, Junyu
Cui, Jinjie
Gao, Xueke
author_facet Du, Lingen
Xue, Hui
Hu, Fangmei
Zhu, Xiangzhen
Wang, Li
Zhang, Kaixin
Li, Dongyang
Ji, Jichao
Niu, Lin
Luo, Junyu
Cui, Jinjie
Gao, Xueke
author_sort Du, Lingen
collection PubMed
description INTRODUCTION: Bacteria play critical roles in the reproduction, metabolism, physiology, and detoxification of their insect hosts. The ladybird beetle (Harmonia axyridis) harbors a myriad of endosymbiotic microbes. However, to date, little is known about how the microbial composition of H. axyridis varies throughout its life cycle. METHODS: In this study, 16S rRNA amplicon sequencing and quantitative PCR were employed to investigate the diversity and dynamics of bacterial symbionts across the egg, larval, pupae, and adults stages of H. axyridis. RESULTS: Higher bacterial community richness and diversity were observed in eggs, followed by those in adults and pupae. The community richness index differed significantly between second-instar larvae and other developmental stages. Proteobacteria, Firmicutes, and Actinobacteria were the dominant phyla. Staphylococcus, Enterobacter, Glutamicibacter, and Acinetobacter were the dominant bacteria genera; however, their relative abundances fluctuated across host developmental stages. Interestingly, the larval stage harbored high proportions of Firmicutes, whereas the adult microbial community largely consisted of Proteobacteria. DISCUSSION: This study is the first to determine the symbiotic bacterial composition across key life stages of H. axyridis. These outcomes can foster the development of environmental risk assessments and novel biological control strategies.
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spelling pubmed-97519982022-12-16 Dynamics of symbiotic bacterial community in whole life stage of Harmonia axyridis (Coleoptera: Coccinellidae) Du, Lingen Xue, Hui Hu, Fangmei Zhu, Xiangzhen Wang, Li Zhang, Kaixin Li, Dongyang Ji, Jichao Niu, Lin Luo, Junyu Cui, Jinjie Gao, Xueke Front Microbiol Microbiology INTRODUCTION: Bacteria play critical roles in the reproduction, metabolism, physiology, and detoxification of their insect hosts. The ladybird beetle (Harmonia axyridis) harbors a myriad of endosymbiotic microbes. However, to date, little is known about how the microbial composition of H. axyridis varies throughout its life cycle. METHODS: In this study, 16S rRNA amplicon sequencing and quantitative PCR were employed to investigate the diversity and dynamics of bacterial symbionts across the egg, larval, pupae, and adults stages of H. axyridis. RESULTS: Higher bacterial community richness and diversity were observed in eggs, followed by those in adults and pupae. The community richness index differed significantly between second-instar larvae and other developmental stages. Proteobacteria, Firmicutes, and Actinobacteria were the dominant phyla. Staphylococcus, Enterobacter, Glutamicibacter, and Acinetobacter were the dominant bacteria genera; however, their relative abundances fluctuated across host developmental stages. Interestingly, the larval stage harbored high proportions of Firmicutes, whereas the adult microbial community largely consisted of Proteobacteria. DISCUSSION: This study is the first to determine the symbiotic bacterial composition across key life stages of H. axyridis. These outcomes can foster the development of environmental risk assessments and novel biological control strategies. Frontiers Media S.A. 2022-12-01 /pmc/articles/PMC9751998/ /pubmed/36532478 http://dx.doi.org/10.3389/fmicb.2022.1050329 Text en Copyright © 2022 Du, Xue, Hu, Zhu, Wang, Zhang, Li, Ji, Niu, Luo, Cui and Gao. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Microbiology
Du, Lingen
Xue, Hui
Hu, Fangmei
Zhu, Xiangzhen
Wang, Li
Zhang, Kaixin
Li, Dongyang
Ji, Jichao
Niu, Lin
Luo, Junyu
Cui, Jinjie
Gao, Xueke
Dynamics of symbiotic bacterial community in whole life stage of Harmonia axyridis (Coleoptera: Coccinellidae)
title Dynamics of symbiotic bacterial community in whole life stage of Harmonia axyridis (Coleoptera: Coccinellidae)
title_full Dynamics of symbiotic bacterial community in whole life stage of Harmonia axyridis (Coleoptera: Coccinellidae)
title_fullStr Dynamics of symbiotic bacterial community in whole life stage of Harmonia axyridis (Coleoptera: Coccinellidae)
title_full_unstemmed Dynamics of symbiotic bacterial community in whole life stage of Harmonia axyridis (Coleoptera: Coccinellidae)
title_short Dynamics of symbiotic bacterial community in whole life stage of Harmonia axyridis (Coleoptera: Coccinellidae)
title_sort dynamics of symbiotic bacterial community in whole life stage of harmonia axyridis (coleoptera: coccinellidae)
topic Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9751998/
https://www.ncbi.nlm.nih.gov/pubmed/36532478
http://dx.doi.org/10.3389/fmicb.2022.1050329
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