Cargando…
2308. A Nationwide Surveillance of the Clinical Presentation and Genomic Characteristics of Listeria monocytogenes Infections in Japan; Preliminary data
BACKGROUND: Listeria monocytogenes is the cause of listeriosis and is associated with foodborne outbreak and severe diseases in immunocompromised persons. However, the clinical and genomic characteristics of listeriosis remains unclear in Japan. We describe preliminary findings from a nationwide sur...
Autores principales: | , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Oxford University Press
2022
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9752142/ http://dx.doi.org/10.1093/ofid/ofac492.140 |
_version_ | 1784850647533748224 |
---|---|
author | Shinohara, Koh Tsuchido, Yasuhiro Yukawa, Satomi Noguchi, Taro Yamamoto, Masaki Matsumura, Yasufumi Nagao, Miki |
author_facet | Shinohara, Koh Tsuchido, Yasuhiro Yukawa, Satomi Noguchi, Taro Yamamoto, Masaki Matsumura, Yasufumi Nagao, Miki |
author_sort | Shinohara, Koh |
collection | PubMed |
description | BACKGROUND: Listeria monocytogenes is the cause of listeriosis and is associated with foodborne outbreak and severe diseases in immunocompromised persons. However, the clinical and genomic characteristics of listeriosis remains unclear in Japan. We describe preliminary findings from a nationwide surveillance of patients and isolates of listeriosis in Japan over 11-year period. METHODS: We conducted a nationwide, multi-center retrospective study of consecutive cases with a confirmed diagnosis of listeriosis between 2011 and 2021. 25 hospitals participated in this study. Clinical characteristics, treatment and outcome were retrieved from the medical records. The preserved L. monocytogenes isolates were investigated with molecular analysis using whole genome sequencing. RESULTS: As of April 2022, a total of 113 cases from 16 hospitals and 26 isolates from 7 hospitals were included in this study. Sixty-one (54%) were male and median age was 74.0 (interquartile range: 64.0 – 82.0). Ten (9%) perinatal infections, 66 (58%) cases of bacteremia, 29 (26%) cases of neurolisteriosis, and eight (7%) other infections were included. In eight episodes with ten perinatal cases, four (50%) episodes experienced fetal loss or neonatal deaths. In 103 non-perinatal cases, 56 (54%) cases received immunosuppressive therapy and in-hospital mortality rate was 17.0%. In 29 neurolisteriosis cases, 13 (45%) cases were associated with poor outcome (mortality or neurological sequelae). In 26 isolates, from two perinatal infections, 13 bacteremia, 9 neurolisteriosis and two other infections, clonal complex (CC) 1, CC2 and CC3 were predominant and accounted for 18 (69%) isolates (Figure). CC1 (5/8 isolates) and CC3 (2/6 isolates) were predominantly found in neurolisteriosis. Variations of the presence of several virulence factors associated with adherence, invasion, and exotoxin were observed. CC1 and CC2 lacked ActA and Ami, whereas Listeriolysin S (LLS) were seen in CC1, CC3 and CC4. [Figure: see text] CONCLUSION: In Japan, most cases of listeriosis are sporadically seen in elderly patients with co-morbidities, instead of foodborne outbreak. Outcomes of neurolisteriosis and perinatal listeriosis were poor. CC1 and CC3, which harbored LLS, were associated with neurolisteriosis. DISCLOSURES: All Authors: No reported disclosures. |
format | Online Article Text |
id | pubmed-9752142 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Oxford University Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-97521422022-12-16 2308. A Nationwide Surveillance of the Clinical Presentation and Genomic Characteristics of Listeria monocytogenes Infections in Japan; Preliminary data Shinohara, Koh Tsuchido, Yasuhiro Yukawa, Satomi Noguchi, Taro Yamamoto, Masaki Matsumura, Yasufumi Nagao, Miki Open Forum Infect Dis Abstracts BACKGROUND: Listeria monocytogenes is the cause of listeriosis and is associated with foodborne outbreak and severe diseases in immunocompromised persons. However, the clinical and genomic characteristics of listeriosis remains unclear in Japan. We describe preliminary findings from a nationwide surveillance of patients and isolates of listeriosis in Japan over 11-year period. METHODS: We conducted a nationwide, multi-center retrospective study of consecutive cases with a confirmed diagnosis of listeriosis between 2011 and 2021. 25 hospitals participated in this study. Clinical characteristics, treatment and outcome were retrieved from the medical records. The preserved L. monocytogenes isolates were investigated with molecular analysis using whole genome sequencing. RESULTS: As of April 2022, a total of 113 cases from 16 hospitals and 26 isolates from 7 hospitals were included in this study. Sixty-one (54%) were male and median age was 74.0 (interquartile range: 64.0 – 82.0). Ten (9%) perinatal infections, 66 (58%) cases of bacteremia, 29 (26%) cases of neurolisteriosis, and eight (7%) other infections were included. In eight episodes with ten perinatal cases, four (50%) episodes experienced fetal loss or neonatal deaths. In 103 non-perinatal cases, 56 (54%) cases received immunosuppressive therapy and in-hospital mortality rate was 17.0%. In 29 neurolisteriosis cases, 13 (45%) cases were associated with poor outcome (mortality or neurological sequelae). In 26 isolates, from two perinatal infections, 13 bacteremia, 9 neurolisteriosis and two other infections, clonal complex (CC) 1, CC2 and CC3 were predominant and accounted for 18 (69%) isolates (Figure). CC1 (5/8 isolates) and CC3 (2/6 isolates) were predominantly found in neurolisteriosis. Variations of the presence of several virulence factors associated with adherence, invasion, and exotoxin were observed. CC1 and CC2 lacked ActA and Ami, whereas Listeriolysin S (LLS) were seen in CC1, CC3 and CC4. [Figure: see text] CONCLUSION: In Japan, most cases of listeriosis are sporadically seen in elderly patients with co-morbidities, instead of foodborne outbreak. Outcomes of neurolisteriosis and perinatal listeriosis were poor. CC1 and CC3, which harbored LLS, were associated with neurolisteriosis. DISCLOSURES: All Authors: No reported disclosures. Oxford University Press 2022-12-15 /pmc/articles/PMC9752142/ http://dx.doi.org/10.1093/ofid/ofac492.140 Text en © The Author(s) 2022. Published by Oxford University Press on behalf of Infectious Diseases Society of America. https://creativecommons.org/licenses/by/4.0/This is an Open Access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Abstracts Shinohara, Koh Tsuchido, Yasuhiro Yukawa, Satomi Noguchi, Taro Yamamoto, Masaki Matsumura, Yasufumi Nagao, Miki 2308. A Nationwide Surveillance of the Clinical Presentation and Genomic Characteristics of Listeria monocytogenes Infections in Japan; Preliminary data |
title | 2308. A Nationwide Surveillance of the Clinical Presentation and Genomic Characteristics of Listeria monocytogenes Infections in Japan; Preliminary data |
title_full | 2308. A Nationwide Surveillance of the Clinical Presentation and Genomic Characteristics of Listeria monocytogenes Infections in Japan; Preliminary data |
title_fullStr | 2308. A Nationwide Surveillance of the Clinical Presentation and Genomic Characteristics of Listeria monocytogenes Infections in Japan; Preliminary data |
title_full_unstemmed | 2308. A Nationwide Surveillance of the Clinical Presentation and Genomic Characteristics of Listeria monocytogenes Infections in Japan; Preliminary data |
title_short | 2308. A Nationwide Surveillance of the Clinical Presentation and Genomic Characteristics of Listeria monocytogenes Infections in Japan; Preliminary data |
title_sort | 2308. a nationwide surveillance of the clinical presentation and genomic characteristics of listeria monocytogenes infections in japan; preliminary data |
topic | Abstracts |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9752142/ http://dx.doi.org/10.1093/ofid/ofac492.140 |
work_keys_str_mv | AT shinoharakoh 2308anationwidesurveillanceoftheclinicalpresentationandgenomiccharacteristicsoflisteriamonocytogenesinfectionsinjapanpreliminarydata AT tsuchidoyasuhiro 2308anationwidesurveillanceoftheclinicalpresentationandgenomiccharacteristicsoflisteriamonocytogenesinfectionsinjapanpreliminarydata AT yukawasatomi 2308anationwidesurveillanceoftheclinicalpresentationandgenomiccharacteristicsoflisteriamonocytogenesinfectionsinjapanpreliminarydata AT noguchitaro 2308anationwidesurveillanceoftheclinicalpresentationandgenomiccharacteristicsoflisteriamonocytogenesinfectionsinjapanpreliminarydata AT yamamotomasaki 2308anationwidesurveillanceoftheclinicalpresentationandgenomiccharacteristicsoflisteriamonocytogenesinfectionsinjapanpreliminarydata AT matsumurayasufumi 2308anationwidesurveillanceoftheclinicalpresentationandgenomiccharacteristicsoflisteriamonocytogenesinfectionsinjapanpreliminarydata AT nagaomiki 2308anationwidesurveillanceoftheclinicalpresentationandgenomiccharacteristicsoflisteriamonocytogenesinfectionsinjapanpreliminarydata |