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135. Comparing the Bioinformatic and Experimental Approaches for Identifying Antibiotic Resistance Genes on Plasmids of Acinetobacter baumannii

BACKGROUND: Antimicrobial resistance (AMR) genes of bacteria can be found in chromosomes or plasmids. Due to horizontal transfer from one bacterium to another, AMR genes on plasmids can be easily spread. However, distinguishing whether AMR genes are from plasmids or chromosomes is difficult. Here, w...

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Autores principales: Hwang, Munok, Choi, Hosoon, Jinadatha, Chetan, Xu, Jing, Navarathna, Thanuri, Ashby, Landon, Bennett, Morgan, Kaye, Keith S, Dhar, Sorabh, Chatterjee, Piyali
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9752305/
http://dx.doi.org/10.1093/ofid/ofac492.213
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author Hwang, Munok
Choi, Hosoon
Jinadatha, Chetan
Xu, Jing
Navarathna, Thanuri
Ashby, Landon
Bennett, Morgan
Kaye, Keith S
Dhar, Sorabh
Chatterjee, Piyali
author_facet Hwang, Munok
Choi, Hosoon
Jinadatha, Chetan
Xu, Jing
Navarathna, Thanuri
Ashby, Landon
Bennett, Morgan
Kaye, Keith S
Dhar, Sorabh
Chatterjee, Piyali
author_sort Hwang, Munok
collection PubMed
description BACKGROUND: Antimicrobial resistance (AMR) genes of bacteria can be found in chromosomes or plasmids. Due to horizontal transfer from one bacterium to another, AMR genes on plasmids can be easily spread. However, distinguishing whether AMR genes are from plasmids or chromosomes is difficult. Here, we assessed two different approaches for identifying AMR genes on the plasmids of A. baumannii. [Figure: see text] METHODS: Genomic DNA from 30 clinical isolates was sequenced using Illumina NextSeq 550. Two approaches were applied to identify which AMR genes were on plasmids: isolation of plasmid DNA experimentally using plasmid isolation kit (Qiagen, CA), and deduction of plasmid sequence from genomic DNA using the bioinformatic tool, plasmidSPAdes, which distinguishes the plasmid sequence based on the copy number. AMR genes from both approaches were identified using the open-source AMR database, ResFinder. The AMR gene data obtained from plasmids utilizing each approach were compared based on information from whole genome sequencing (WGS) data. RESULTS: As shown in Table 1, both approaches produced differences in the number of contigs, largest contig size, and number of AMR genes identified. The average number of contigs using plasmidSPAdes with WGS data was 7.4 and the largest contig size was 52939 base pairs, while with isolated plasmid DNA and analyzed by SPAdes was 1025 and 32274 bp, respectively. Plasmid DNA contigs are more fragmented. The average number of AMR genes identified using plasmidSPAdes was 1.2 and using plasmid DNA was 4. AMR information from WGS data showed 73.3% isolates have blaOXA-23, a carbapenem resistant gene. However, blaOXA-23 was found from 40% isolates using plasmidSpades and 50% isolates using plasmid DNA. In 36.7% isolates, blaOXA-23 was found by both approaches. CONCLUSION: The information obtained by the two approaches for identifying resistance genes on plasmids mostly agreed with some exceptions. The bla OXA-23 identified in both could be on plasmids, while genes not identified from both could be on chromosomes. PlasmidSPAdes was a useful tool to discriminate plasmid sequence from WGS data. Stringent infection control measures can prevent spread of AMR gene containing plasmids in hospital settings. DISCLOSURES: Chetan Jinadatha, MD, MPH, AHRQ R01 Grant-5R01HS025598: Grant/Research Support|EOS Surfaces: Copper Coupons and materials for testing Keith S. Kaye, MD, MPH, Allecra: Advisor/Consultant|GlaxoSmithKline plc.: Receiving symposia honoraria|GlaxoSmithKline plc.: GlaxoSmithKline plc.-sponsored study 212502|Merck: Advisor/Consultant|qpex: Advisor/Consultant|Shionogi: Grant/Research Support|Spero: Advisor/Consultant Piyali Chatterjee, PhD, AHRQ Grant # 1R03HS027667-01: Grant/Research Support|AHRQ Grant # 1R03HS027667-01: Central Texas Veterans Health Care System.
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spelling pubmed-97523052022-12-16 135. Comparing the Bioinformatic and Experimental Approaches for Identifying Antibiotic Resistance Genes on Plasmids of Acinetobacter baumannii Hwang, Munok Choi, Hosoon Jinadatha, Chetan Xu, Jing Navarathna, Thanuri Ashby, Landon Bennett, Morgan Kaye, Keith S Dhar, Sorabh Chatterjee, Piyali Open Forum Infect Dis Abstracts BACKGROUND: Antimicrobial resistance (AMR) genes of bacteria can be found in chromosomes or plasmids. Due to horizontal transfer from one bacterium to another, AMR genes on plasmids can be easily spread. However, distinguishing whether AMR genes are from plasmids or chromosomes is difficult. Here, we assessed two different approaches for identifying AMR genes on the plasmids of A. baumannii. [Figure: see text] METHODS: Genomic DNA from 30 clinical isolates was sequenced using Illumina NextSeq 550. Two approaches were applied to identify which AMR genes were on plasmids: isolation of plasmid DNA experimentally using plasmid isolation kit (Qiagen, CA), and deduction of plasmid sequence from genomic DNA using the bioinformatic tool, plasmidSPAdes, which distinguishes the plasmid sequence based on the copy number. AMR genes from both approaches were identified using the open-source AMR database, ResFinder. The AMR gene data obtained from plasmids utilizing each approach were compared based on information from whole genome sequencing (WGS) data. RESULTS: As shown in Table 1, both approaches produced differences in the number of contigs, largest contig size, and number of AMR genes identified. The average number of contigs using plasmidSPAdes with WGS data was 7.4 and the largest contig size was 52939 base pairs, while with isolated plasmid DNA and analyzed by SPAdes was 1025 and 32274 bp, respectively. Plasmid DNA contigs are more fragmented. The average number of AMR genes identified using plasmidSPAdes was 1.2 and using plasmid DNA was 4. AMR information from WGS data showed 73.3% isolates have blaOXA-23, a carbapenem resistant gene. However, blaOXA-23 was found from 40% isolates using plasmidSpades and 50% isolates using plasmid DNA. In 36.7% isolates, blaOXA-23 was found by both approaches. CONCLUSION: The information obtained by the two approaches for identifying resistance genes on plasmids mostly agreed with some exceptions. The bla OXA-23 identified in both could be on plasmids, while genes not identified from both could be on chromosomes. PlasmidSPAdes was a useful tool to discriminate plasmid sequence from WGS data. Stringent infection control measures can prevent spread of AMR gene containing plasmids in hospital settings. DISCLOSURES: Chetan Jinadatha, MD, MPH, AHRQ R01 Grant-5R01HS025598: Grant/Research Support|EOS Surfaces: Copper Coupons and materials for testing Keith S. Kaye, MD, MPH, Allecra: Advisor/Consultant|GlaxoSmithKline plc.: Receiving symposia honoraria|GlaxoSmithKline plc.: GlaxoSmithKline plc.-sponsored study 212502|Merck: Advisor/Consultant|qpex: Advisor/Consultant|Shionogi: Grant/Research Support|Spero: Advisor/Consultant Piyali Chatterjee, PhD, AHRQ Grant # 1R03HS027667-01: Grant/Research Support|AHRQ Grant # 1R03HS027667-01: Central Texas Veterans Health Care System. Oxford University Press 2022-12-15 /pmc/articles/PMC9752305/ http://dx.doi.org/10.1093/ofid/ofac492.213 Text en © The Author(s) 2022. Published by Oxford University Press on behalf of Infectious Diseases Society of America. https://creativecommons.org/licenses/by/4.0/This is an Open Access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Abstracts
Hwang, Munok
Choi, Hosoon
Jinadatha, Chetan
Xu, Jing
Navarathna, Thanuri
Ashby, Landon
Bennett, Morgan
Kaye, Keith S
Dhar, Sorabh
Chatterjee, Piyali
135. Comparing the Bioinformatic and Experimental Approaches for Identifying Antibiotic Resistance Genes on Plasmids of Acinetobacter baumannii
title 135. Comparing the Bioinformatic and Experimental Approaches for Identifying Antibiotic Resistance Genes on Plasmids of Acinetobacter baumannii
title_full 135. Comparing the Bioinformatic and Experimental Approaches for Identifying Antibiotic Resistance Genes on Plasmids of Acinetobacter baumannii
title_fullStr 135. Comparing the Bioinformatic and Experimental Approaches for Identifying Antibiotic Resistance Genes on Plasmids of Acinetobacter baumannii
title_full_unstemmed 135. Comparing the Bioinformatic and Experimental Approaches for Identifying Antibiotic Resistance Genes on Plasmids of Acinetobacter baumannii
title_short 135. Comparing the Bioinformatic and Experimental Approaches for Identifying Antibiotic Resistance Genes on Plasmids of Acinetobacter baumannii
title_sort 135. comparing the bioinformatic and experimental approaches for identifying antibiotic resistance genes on plasmids of acinetobacter baumannii
topic Abstracts
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9752305/
http://dx.doi.org/10.1093/ofid/ofac492.213
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