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Carbohydrate-Derived Polytriazole Nanoparticles Enhance the Anti-Inflammatory Activity of Cilostazol

[Image: see text] Poly(amide-triazole) and poly(ester-triazole) synthesized from d-galactose as a renewable resource were applied for the synthesis of nanoparticles (NPs) by the emulsification/solvent evaporation method. The NPs were characterized as stable, spherical particles, and none of their co...

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Autores principales: Rivas, M. Verónica, Musikant, Daniel, Díaz Peña, Rocío, Álvarez, Daniela, Pelazzo, Luciana, Rossi, Ezequiel, Martínez, Karina D., Errea, María I., Pérez, Oscar E., Varela, Oscar, Kolender, Adriana A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Chemical Society 2022
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9753171/
https://www.ncbi.nlm.nih.gov/pubmed/36530317
http://dx.doi.org/10.1021/acsomega.2c02969
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author Rivas, M. Verónica
Musikant, Daniel
Díaz Peña, Rocío
Álvarez, Daniela
Pelazzo, Luciana
Rossi, Ezequiel
Martínez, Karina D.
Errea, María I.
Pérez, Oscar E.
Varela, Oscar
Kolender, Adriana A.
author_facet Rivas, M. Verónica
Musikant, Daniel
Díaz Peña, Rocío
Álvarez, Daniela
Pelazzo, Luciana
Rossi, Ezequiel
Martínez, Karina D.
Errea, María I.
Pérez, Oscar E.
Varela, Oscar
Kolender, Adriana A.
author_sort Rivas, M. Verónica
collection PubMed
description [Image: see text] Poly(amide-triazole) and poly(ester-triazole) synthesized from d-galactose as a renewable resource were applied for the synthesis of nanoparticles (NPs) by the emulsification/solvent evaporation method. The NPs were characterized as stable, spherical particles, and none of their components, including the stabilizer poly(vinyl alcohol), were cytotoxic for normal rat kidney cells. These NPs proved to be useful for the efficient encapsulation of cilostazol (CLZ), an antiplatelet and vasodilator drug currently used for the treatment of intermittent claudication, which is associated with undesired side-effects. In this context, the nanoencapsulation of CLZ was expected to improve its therapeutic administration. The carbohydrate-derived polymeric NPs were designed taking into account that the triazole rings of the polymer backbone could have attractive interactions with the tetrazole ring of CLZ. The activity of the nanoencapsulated CLZ was measured using a matrix metalloproteinase model in a lipopolysaccharide-induced inflammation system. Interestingly, the encapsulated drug exhibited enhanced anti-inflammatory activity in comparison with the free drug. The results are very promising since the stable, noncytotoxic NP systems efficiently reduced the inflammation response at low CLZ doses. In summary, the NPs were obtained through an innovative methodology that combines a carbohydrate-derived synthetic polymer, designed to interact with the drug, ease of preparation, adequate biological performance, and environmentally friendly production.
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spelling pubmed-97531712022-12-16 Carbohydrate-Derived Polytriazole Nanoparticles Enhance the Anti-Inflammatory Activity of Cilostazol Rivas, M. Verónica Musikant, Daniel Díaz Peña, Rocío Álvarez, Daniela Pelazzo, Luciana Rossi, Ezequiel Martínez, Karina D. Errea, María I. Pérez, Oscar E. Varela, Oscar Kolender, Adriana A. ACS Omega [Image: see text] Poly(amide-triazole) and poly(ester-triazole) synthesized from d-galactose as a renewable resource were applied for the synthesis of nanoparticles (NPs) by the emulsification/solvent evaporation method. The NPs were characterized as stable, spherical particles, and none of their components, including the stabilizer poly(vinyl alcohol), were cytotoxic for normal rat kidney cells. These NPs proved to be useful for the efficient encapsulation of cilostazol (CLZ), an antiplatelet and vasodilator drug currently used for the treatment of intermittent claudication, which is associated with undesired side-effects. In this context, the nanoencapsulation of CLZ was expected to improve its therapeutic administration. The carbohydrate-derived polymeric NPs were designed taking into account that the triazole rings of the polymer backbone could have attractive interactions with the tetrazole ring of CLZ. The activity of the nanoencapsulated CLZ was measured using a matrix metalloproteinase model in a lipopolysaccharide-induced inflammation system. Interestingly, the encapsulated drug exhibited enhanced anti-inflammatory activity in comparison with the free drug. The results are very promising since the stable, noncytotoxic NP systems efficiently reduced the inflammation response at low CLZ doses. In summary, the NPs were obtained through an innovative methodology that combines a carbohydrate-derived synthetic polymer, designed to interact with the drug, ease of preparation, adequate biological performance, and environmentally friendly production. American Chemical Society 2022-11-30 /pmc/articles/PMC9753171/ /pubmed/36530317 http://dx.doi.org/10.1021/acsomega.2c02969 Text en © 2022 The Authors. Published by American Chemical Society https://creativecommons.org/licenses/by-nc-nd/4.0/Permits non-commercial access and re-use, provided that author attribution and integrity are maintained; but does not permit creation of adaptations or other derivative works (https://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Rivas, M. Verónica
Musikant, Daniel
Díaz Peña, Rocío
Álvarez, Daniela
Pelazzo, Luciana
Rossi, Ezequiel
Martínez, Karina D.
Errea, María I.
Pérez, Oscar E.
Varela, Oscar
Kolender, Adriana A.
Carbohydrate-Derived Polytriazole Nanoparticles Enhance the Anti-Inflammatory Activity of Cilostazol
title Carbohydrate-Derived Polytriazole Nanoparticles Enhance the Anti-Inflammatory Activity of Cilostazol
title_full Carbohydrate-Derived Polytriazole Nanoparticles Enhance the Anti-Inflammatory Activity of Cilostazol
title_fullStr Carbohydrate-Derived Polytriazole Nanoparticles Enhance the Anti-Inflammatory Activity of Cilostazol
title_full_unstemmed Carbohydrate-Derived Polytriazole Nanoparticles Enhance the Anti-Inflammatory Activity of Cilostazol
title_short Carbohydrate-Derived Polytriazole Nanoparticles Enhance the Anti-Inflammatory Activity of Cilostazol
title_sort carbohydrate-derived polytriazole nanoparticles enhance the anti-inflammatory activity of cilostazol
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9753171/
https://www.ncbi.nlm.nih.gov/pubmed/36530317
http://dx.doi.org/10.1021/acsomega.2c02969
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