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Gut microbiota in the early stage of Crohn’s disease has unique characteristics
BACKGROUND: Emerging evidence suggests that gut microbiota plays a predominant role in Crohn’s disease (CD). However, the microbiome alterations in the early stage of CD patients still remain unclear. The present study aimed to identify dysbacteriosis in patients with early CD and explore specific g...
Autores principales: | , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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BioMed Central
2022
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9753350/ https://www.ncbi.nlm.nih.gov/pubmed/36517872 http://dx.doi.org/10.1186/s13099-022-00521-0 |
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author | Ma, Xianzong Lu, Xiaojuan Zhang, Wenyu Yang, Lang Wang, Dezhi Xu, Junfeng Jia, Yan Wang, Xin Xie, Hui Li, Shu Zhang, Mingjie He, Yuqi Jin, Peng Sheng, Jianqiu |
author_facet | Ma, Xianzong Lu, Xiaojuan Zhang, Wenyu Yang, Lang Wang, Dezhi Xu, Junfeng Jia, Yan Wang, Xin Xie, Hui Li, Shu Zhang, Mingjie He, Yuqi Jin, Peng Sheng, Jianqiu |
author_sort | Ma, Xianzong |
collection | PubMed |
description | BACKGROUND: Emerging evidence suggests that gut microbiota plays a predominant role in Crohn’s disease (CD). However, the microbiome alterations in the early stage of CD patients still remain unclear. The present study aimed to identify dysbacteriosis in patients with early CD and explore specific gut bacteria related to the progression of CD. METHODS: This study was nested within a longitudinal prospective Chinese CD cohort, and it included 18 early CD patients, 22 advanced CD patients and 30 healthy controls. The microbiota communities were investigated using high-throughput Illumina HiSeq sequencing targeting the V3–V4 region of 16S ribosomal DNA (rDNA) gene. The relationship between the gut microbiota and clinical characteristics of CD was analyzed. RESULTS: Differential microbiota compositions were observed in CD samples (including early and advanced CD samples) and healthy controls samples. Notably, Lachnospiracea_incertae_sedis and Parabacteroides were enriched in the early CD patients, Escherichia/Shigella, Enterococcus and Proteus were enriched in the advanced CD patients, and Roseburia, Gemmiger, Coprococcus, Ruminococcus 2, Butyricicoccus, Dorea, Fusicatenibacter, Anaerostipes, Clostridium IV were enriched in the healthy controls [LDA score (log10) > 2]. Furthermore, Kruskal–Wallis Rank sum test results showed that Blautia, Clostridium IV, Coprococcus, Dorea, Fusicatenibacter continued to significantly decrease in early and advanced CD patients, and Escherichia/Shigella and Proteus continued to significantly increase compared with healthy controls (P < 0.05). The PICRUSt analysis identified 16 remarkably different metabolic pathways [LDA score (log10) > 2]. Some genera were significantly correlated with various clinical parameters, such as fecal calprotectin, erythrocyte sedimentation rate, C-reactive protein, gland reduce, goblet cells decreased, clinical symptoms (P < 0.05). CONCLUSIONS: Dysbacteriosis occurs in the early stage of CD and is associated with the progression of CD. This data provides a foundation that furthers the understanding of the role of gut microbiota in CD’s pathogenesis. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s13099-022-00521-0. |
format | Online Article Text |
id | pubmed-9753350 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-97533502022-12-16 Gut microbiota in the early stage of Crohn’s disease has unique characteristics Ma, Xianzong Lu, Xiaojuan Zhang, Wenyu Yang, Lang Wang, Dezhi Xu, Junfeng Jia, Yan Wang, Xin Xie, Hui Li, Shu Zhang, Mingjie He, Yuqi Jin, Peng Sheng, Jianqiu Gut Pathog Research BACKGROUND: Emerging evidence suggests that gut microbiota plays a predominant role in Crohn’s disease (CD). However, the microbiome alterations in the early stage of CD patients still remain unclear. The present study aimed to identify dysbacteriosis in patients with early CD and explore specific gut bacteria related to the progression of CD. METHODS: This study was nested within a longitudinal prospective Chinese CD cohort, and it included 18 early CD patients, 22 advanced CD patients and 30 healthy controls. The microbiota communities were investigated using high-throughput Illumina HiSeq sequencing targeting the V3–V4 region of 16S ribosomal DNA (rDNA) gene. The relationship between the gut microbiota and clinical characteristics of CD was analyzed. RESULTS: Differential microbiota compositions were observed in CD samples (including early and advanced CD samples) and healthy controls samples. Notably, Lachnospiracea_incertae_sedis and Parabacteroides were enriched in the early CD patients, Escherichia/Shigella, Enterococcus and Proteus were enriched in the advanced CD patients, and Roseburia, Gemmiger, Coprococcus, Ruminococcus 2, Butyricicoccus, Dorea, Fusicatenibacter, Anaerostipes, Clostridium IV were enriched in the healthy controls [LDA score (log10) > 2]. Furthermore, Kruskal–Wallis Rank sum test results showed that Blautia, Clostridium IV, Coprococcus, Dorea, Fusicatenibacter continued to significantly decrease in early and advanced CD patients, and Escherichia/Shigella and Proteus continued to significantly increase compared with healthy controls (P < 0.05). The PICRUSt analysis identified 16 remarkably different metabolic pathways [LDA score (log10) > 2]. Some genera were significantly correlated with various clinical parameters, such as fecal calprotectin, erythrocyte sedimentation rate, C-reactive protein, gland reduce, goblet cells decreased, clinical symptoms (P < 0.05). CONCLUSIONS: Dysbacteriosis occurs in the early stage of CD and is associated with the progression of CD. This data provides a foundation that furthers the understanding of the role of gut microbiota in CD’s pathogenesis. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s13099-022-00521-0. BioMed Central 2022-12-14 /pmc/articles/PMC9753350/ /pubmed/36517872 http://dx.doi.org/10.1186/s13099-022-00521-0 Text en © The Author(s) 2022 https://creativecommons.org/licenses/by/4.0/Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/ (https://creativecommons.org/publicdomain/zero/1.0/) ) applies to the data made available in this article, unless otherwise stated in a credit line to the data. |
spellingShingle | Research Ma, Xianzong Lu, Xiaojuan Zhang, Wenyu Yang, Lang Wang, Dezhi Xu, Junfeng Jia, Yan Wang, Xin Xie, Hui Li, Shu Zhang, Mingjie He, Yuqi Jin, Peng Sheng, Jianqiu Gut microbiota in the early stage of Crohn’s disease has unique characteristics |
title | Gut microbiota in the early stage of Crohn’s disease has unique characteristics |
title_full | Gut microbiota in the early stage of Crohn’s disease has unique characteristics |
title_fullStr | Gut microbiota in the early stage of Crohn’s disease has unique characteristics |
title_full_unstemmed | Gut microbiota in the early stage of Crohn’s disease has unique characteristics |
title_short | Gut microbiota in the early stage of Crohn’s disease has unique characteristics |
title_sort | gut microbiota in the early stage of crohn’s disease has unique characteristics |
topic | Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9753350/ https://www.ncbi.nlm.nih.gov/pubmed/36517872 http://dx.doi.org/10.1186/s13099-022-00521-0 |
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