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Oxidative stress biomarkers in dogs with benign prostatic hyperplasia

BACKGROUND: The aim of this study was to evaluate total antioxidant capacity (TAC) and biomarkers of lipid and protein peroxidation in the blood serum of dogs with benign prostatic hyperplasia (BPH). The study was conducted on 36 intact male dogs of various breeds. The dogs were assigned to two grou...

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Autores principales: Domoslawska, Anna, Zduńczyk, Sławomir, Kankofer, Marta, Bielecka, Arleta
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9753414/
https://www.ncbi.nlm.nih.gov/pubmed/36517914
http://dx.doi.org/10.1186/s13620-022-00228-3
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author Domoslawska, Anna
Zduńczyk, Sławomir
Kankofer, Marta
Bielecka, Arleta
author_facet Domoslawska, Anna
Zduńczyk, Sławomir
Kankofer, Marta
Bielecka, Arleta
author_sort Domoslawska, Anna
collection PubMed
description BACKGROUND: The aim of this study was to evaluate total antioxidant capacity (TAC) and biomarkers of lipid and protein peroxidation in the blood serum of dogs with benign prostatic hyperplasia (BPH). The study was conducted on 36 intact male dogs of various breeds. The dogs were assigned to two groups: BPH group (n = 18) and non-affected group (n = 18). Blood samples were collected from the cephalic vein. The antioxidant status of the serum was assessed using TAC. The levels of bityrosine, formylkynurenine and SH-groups were used as protein peroxidation biomarkers and the level of radical cations of N,N-diethyl-paraphenylene diamine (RC-DEPPD) was used as a marker for lipid peroxidation. TAC and the concentrations of SH-groups and RC-DEPPD in the serum were determined spectrophotometrically, the concentrations of bityrosine and formylokynurenine, were determined using spectrofluorimetric methods. RESULTS: The mean value of TAC in the serum was significantly lower (P = 0.01) in BPH dogs than in non-affected dogs (3.10 ± 0.56 vs 4.20 ± 1.60 μmol/g protein). Mean levels of protein and lipid oxidation biomarkers showed a trend towards oxidative imbalance, but there were no statistically significant differences between dogs with BPH and controls (P > 0.05). CONCLUSION: In conclusion, significantly lower serum TAC in dogs with BPH compared to non-affected dogs suggests a potential involvement of oxidative stress in the pathogenesis of BPH in dogs. More studies are needed to clarify the role of oxidative stress in the development of BPH in dogs.
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spelling pubmed-97534142022-12-16 Oxidative stress biomarkers in dogs with benign prostatic hyperplasia Domoslawska, Anna Zduńczyk, Sławomir Kankofer, Marta Bielecka, Arleta Ir Vet J Research BACKGROUND: The aim of this study was to evaluate total antioxidant capacity (TAC) and biomarkers of lipid and protein peroxidation in the blood serum of dogs with benign prostatic hyperplasia (BPH). The study was conducted on 36 intact male dogs of various breeds. The dogs were assigned to two groups: BPH group (n = 18) and non-affected group (n = 18). Blood samples were collected from the cephalic vein. The antioxidant status of the serum was assessed using TAC. The levels of bityrosine, formylkynurenine and SH-groups were used as protein peroxidation biomarkers and the level of radical cations of N,N-diethyl-paraphenylene diamine (RC-DEPPD) was used as a marker for lipid peroxidation. TAC and the concentrations of SH-groups and RC-DEPPD in the serum were determined spectrophotometrically, the concentrations of bityrosine and formylokynurenine, were determined using spectrofluorimetric methods. RESULTS: The mean value of TAC in the serum was significantly lower (P = 0.01) in BPH dogs than in non-affected dogs (3.10 ± 0.56 vs 4.20 ± 1.60 μmol/g protein). Mean levels of protein and lipid oxidation biomarkers showed a trend towards oxidative imbalance, but there were no statistically significant differences between dogs with BPH and controls (P > 0.05). CONCLUSION: In conclusion, significantly lower serum TAC in dogs with BPH compared to non-affected dogs suggests a potential involvement of oxidative stress in the pathogenesis of BPH in dogs. More studies are needed to clarify the role of oxidative stress in the development of BPH in dogs. BioMed Central 2022-12-15 /pmc/articles/PMC9753414/ /pubmed/36517914 http://dx.doi.org/10.1186/s13620-022-00228-3 Text en © The Author(s) 2022 https://creativecommons.org/licenses/by/4.0/Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/ (https://creativecommons.org/publicdomain/zero/1.0/) ) applies to the data made available in this article, unless otherwise stated in a credit line to the data.
spellingShingle Research
Domoslawska, Anna
Zduńczyk, Sławomir
Kankofer, Marta
Bielecka, Arleta
Oxidative stress biomarkers in dogs with benign prostatic hyperplasia
title Oxidative stress biomarkers in dogs with benign prostatic hyperplasia
title_full Oxidative stress biomarkers in dogs with benign prostatic hyperplasia
title_fullStr Oxidative stress biomarkers in dogs with benign prostatic hyperplasia
title_full_unstemmed Oxidative stress biomarkers in dogs with benign prostatic hyperplasia
title_short Oxidative stress biomarkers in dogs with benign prostatic hyperplasia
title_sort oxidative stress biomarkers in dogs with benign prostatic hyperplasia
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9753414/
https://www.ncbi.nlm.nih.gov/pubmed/36517914
http://dx.doi.org/10.1186/s13620-022-00228-3
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