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First detection and molecular characteristics of bopivirus from goats in China

A metavirome analysis was performed and detected bopivirus in the diarrhoeal fecal samples of goats in China. A total of 136 fecal samples were collected from yeanlings between the dates of June 2021 and January 2022 in Sichuan province, China. Moreover, “Bopivirus B” strains were detected by a spec...

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Autores principales: Yang, Youwen, Abi, Kehamo, Li, Yanmin, Yang, Chen, Yang, Falong
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9753977/
https://www.ncbi.nlm.nih.gov/pubmed/36532341
http://dx.doi.org/10.3389/fvets.2022.1033011
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author Yang, Youwen
Abi, Kehamo
Li, Yanmin
Yang, Chen
Yang, Falong
author_facet Yang, Youwen
Abi, Kehamo
Li, Yanmin
Yang, Chen
Yang, Falong
author_sort Yang, Youwen
collection PubMed
description A metavirome analysis was performed and detected bopivirus in the diarrhoeal fecal samples of goats in China. A total of 136 fecal samples were collected from yeanlings between the dates of June 2021 and January 2022 in Sichuan province, China. Moreover, “Bopivirus B” strains were detected by a specific RT-PCR targeting the 3D gene of the virus. The results showed that the overall detection rate of “Bopivirus B” was 19.12% (26/136). Additionally, there was a higher detection rate (24.05%, 19/79) in the fecal samples collected from yeanlings with diarrhea compared to those from asymptomatic animals (12.28%, 7/57). In these samples, no other common diarrhea-causing pathogens were detected except for three enteric viruses, namely caprine enterovirus, caprine kobuvirus and caprine hunnivirus (with detection rates of 13.97, 13.97, and 8.82%, respectively). Subsequently, full-length VP4, VP2, VP3, and VP1 genes from “Bopivirus B”-positive samples were amplified, cloned, sequenced, and analyzed. The phylogenetic analysis performed on the VP1 genes revealed that the identified bopivirus belonged to genotype B1 (seven strains) and B2 (three strains) and presented a high genetic diversity. Furthermore, a complete genome sequence of a “Bopivirus B” strain (SWUN/B1/2022) was obtained using PCR from fecal sample of a diarrhoeal yeanling. The complete genome was 7,309 nucleotides in length with a standard picornavirus genome organization, and shares 93.10% and 91.10% nucleotide similarity with bopivirus B1 genotype strain ovine/TB14/2010-HUN and bopivirus B2 genotype strain goat/AGK16/2020-HUN, respectively. According to the species classification criteria put forward by the International Committee on Taxonomy of Viruses and VP1 genotype, the strain SWUN/B1/2022 belongs to the bopivirus B1. This strain has unique amino acid substitutions in the VP4, VP2, VP3, and VP1 genes. Moreover, genomic recombination analysis revealed that this strain may be a minor parental strain of bopivirus B1 ovine/TB14/2010-HUN. Evolutionary analysis based on the 2C and 3CD genes revealed that the new bopivirus B1 strain SWUN/B1/2022 presents a unique evolutionary pattern. This study provided evidence to suggest that “Bopivirus B” is circulating with substantial genetic diversity in goats in China at present, and the mixed infection of “Bopivirus B” with other enteric viruses should be considered to be a composite factor in the occurrence of viral diarrhea in goats.
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spelling pubmed-97539772022-12-16 First detection and molecular characteristics of bopivirus from goats in China Yang, Youwen Abi, Kehamo Li, Yanmin Yang, Chen Yang, Falong Front Vet Sci Veterinary Science A metavirome analysis was performed and detected bopivirus in the diarrhoeal fecal samples of goats in China. A total of 136 fecal samples were collected from yeanlings between the dates of June 2021 and January 2022 in Sichuan province, China. Moreover, “Bopivirus B” strains were detected by a specific RT-PCR targeting the 3D gene of the virus. The results showed that the overall detection rate of “Bopivirus B” was 19.12% (26/136). Additionally, there was a higher detection rate (24.05%, 19/79) in the fecal samples collected from yeanlings with diarrhea compared to those from asymptomatic animals (12.28%, 7/57). In these samples, no other common diarrhea-causing pathogens were detected except for three enteric viruses, namely caprine enterovirus, caprine kobuvirus and caprine hunnivirus (with detection rates of 13.97, 13.97, and 8.82%, respectively). Subsequently, full-length VP4, VP2, VP3, and VP1 genes from “Bopivirus B”-positive samples were amplified, cloned, sequenced, and analyzed. The phylogenetic analysis performed on the VP1 genes revealed that the identified bopivirus belonged to genotype B1 (seven strains) and B2 (three strains) and presented a high genetic diversity. Furthermore, a complete genome sequence of a “Bopivirus B” strain (SWUN/B1/2022) was obtained using PCR from fecal sample of a diarrhoeal yeanling. The complete genome was 7,309 nucleotides in length with a standard picornavirus genome organization, and shares 93.10% and 91.10% nucleotide similarity with bopivirus B1 genotype strain ovine/TB14/2010-HUN and bopivirus B2 genotype strain goat/AGK16/2020-HUN, respectively. According to the species classification criteria put forward by the International Committee on Taxonomy of Viruses and VP1 genotype, the strain SWUN/B1/2022 belongs to the bopivirus B1. This strain has unique amino acid substitutions in the VP4, VP2, VP3, and VP1 genes. Moreover, genomic recombination analysis revealed that this strain may be a minor parental strain of bopivirus B1 ovine/TB14/2010-HUN. Evolutionary analysis based on the 2C and 3CD genes revealed that the new bopivirus B1 strain SWUN/B1/2022 presents a unique evolutionary pattern. This study provided evidence to suggest that “Bopivirus B” is circulating with substantial genetic diversity in goats in China at present, and the mixed infection of “Bopivirus B” with other enteric viruses should be considered to be a composite factor in the occurrence of viral diarrhea in goats. Frontiers Media S.A. 2022-12-01 /pmc/articles/PMC9753977/ /pubmed/36532341 http://dx.doi.org/10.3389/fvets.2022.1033011 Text en Copyright © 2022 Yang, Abi, Li, Yang and Yang. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Veterinary Science
Yang, Youwen
Abi, Kehamo
Li, Yanmin
Yang, Chen
Yang, Falong
First detection and molecular characteristics of bopivirus from goats in China
title First detection and molecular characteristics of bopivirus from goats in China
title_full First detection and molecular characteristics of bopivirus from goats in China
title_fullStr First detection and molecular characteristics of bopivirus from goats in China
title_full_unstemmed First detection and molecular characteristics of bopivirus from goats in China
title_short First detection and molecular characteristics of bopivirus from goats in China
title_sort first detection and molecular characteristics of bopivirus from goats in china
topic Veterinary Science
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9753977/
https://www.ncbi.nlm.nih.gov/pubmed/36532341
http://dx.doi.org/10.3389/fvets.2022.1033011
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