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Frequent exacerbators of chronic obstructive pulmonary disease have distinguishable sputum microbiome signatures during clinical stability

INTRODUCTION: Frequent exacerbation phenotype of chronic obstructive pulmonary disease (COPD) represents a more concerning disease subgroup requiring better prevention and intervention, of which airway microbiome provides new perspective for further exploration. METHODS: To investigate whether frequ...

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Autores principales: Dang, Xiaomin, Kang, Yongyong, Wang, Xiaojian, Cao, Wen, Li, Minhui, He, Ying, Pan, Xinjie, Ye, Kai, Xu, Dan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9753979/
https://www.ncbi.nlm.nih.gov/pubmed/36532417
http://dx.doi.org/10.3389/fmicb.2022.1037037
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author Dang, Xiaomin
Kang, Yongyong
Wang, Xiaojian
Cao, Wen
Li, Minhui
He, Ying
Pan, Xinjie
Ye, Kai
Xu, Dan
author_facet Dang, Xiaomin
Kang, Yongyong
Wang, Xiaojian
Cao, Wen
Li, Minhui
He, Ying
Pan, Xinjie
Ye, Kai
Xu, Dan
author_sort Dang, Xiaomin
collection PubMed
description INTRODUCTION: Frequent exacerbation phenotype of chronic obstructive pulmonary disease (COPD) represents a more concerning disease subgroup requiring better prevention and intervention, of which airway microbiome provides new perspective for further exploration. METHODS: To investigate whether frequent exacerbators of COPD have distinguishable sputum microbiome during clinical stability, COPD patients at high disease grades with or without frequent exacerbation were recruited for sputum microbiome analysis. Sputum samples were collected during clinical stability and underwent 16S rRNA sequencing, which was then subjected for amplicon sequence variants (ASVs)-based microbiome analysis. RESULTS: Our results revealed that compared with healthy controls and infrequent exacerbators, frequent COPD exacerbators have distinguishably dysbiotic sputum microbiome, as featured by fewer ASVs features, lower alpha diversity, distinct beta diversity patterns. Further taxonomic compositional analysis illustrated the structural distinctions between frequent COPD exacerbators and infrequent exacerbators at differential taxa levels and highlighted Stenotrephomonas due to its prominent elevation in frequent COPD exacerbators, providing a promising candidate for further exploration of microbiome biomarker. Moreover, we also demonstrated that frequent exacerbation phenotype is distinguishable from infrequent exacerbation phenotype with respect of functional implications. CONCLUSION: Our study demonstrated the first positive correlation between the frequent exacerbation phenotype of COPD and the sputum microbiome during clinical stability in a single-center Chinese COPD cohort and provide potential diagnostic and therapeutic targets for further investigation.
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spelling pubmed-97539792022-12-16 Frequent exacerbators of chronic obstructive pulmonary disease have distinguishable sputum microbiome signatures during clinical stability Dang, Xiaomin Kang, Yongyong Wang, Xiaojian Cao, Wen Li, Minhui He, Ying Pan, Xinjie Ye, Kai Xu, Dan Front Microbiol Microbiology INTRODUCTION: Frequent exacerbation phenotype of chronic obstructive pulmonary disease (COPD) represents a more concerning disease subgroup requiring better prevention and intervention, of which airway microbiome provides new perspective for further exploration. METHODS: To investigate whether frequent exacerbators of COPD have distinguishable sputum microbiome during clinical stability, COPD patients at high disease grades with or without frequent exacerbation were recruited for sputum microbiome analysis. Sputum samples were collected during clinical stability and underwent 16S rRNA sequencing, which was then subjected for amplicon sequence variants (ASVs)-based microbiome analysis. RESULTS: Our results revealed that compared with healthy controls and infrequent exacerbators, frequent COPD exacerbators have distinguishably dysbiotic sputum microbiome, as featured by fewer ASVs features, lower alpha diversity, distinct beta diversity patterns. Further taxonomic compositional analysis illustrated the structural distinctions between frequent COPD exacerbators and infrequent exacerbators at differential taxa levels and highlighted Stenotrephomonas due to its prominent elevation in frequent COPD exacerbators, providing a promising candidate for further exploration of microbiome biomarker. Moreover, we also demonstrated that frequent exacerbation phenotype is distinguishable from infrequent exacerbation phenotype with respect of functional implications. CONCLUSION: Our study demonstrated the first positive correlation between the frequent exacerbation phenotype of COPD and the sputum microbiome during clinical stability in a single-center Chinese COPD cohort and provide potential diagnostic and therapeutic targets for further investigation. Frontiers Media S.A. 2022-12-01 /pmc/articles/PMC9753979/ /pubmed/36532417 http://dx.doi.org/10.3389/fmicb.2022.1037037 Text en Copyright © 2022 Dang, Kang, Wang, Cao, Li, He, Pan, Ye and Xu. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Microbiology
Dang, Xiaomin
Kang, Yongyong
Wang, Xiaojian
Cao, Wen
Li, Minhui
He, Ying
Pan, Xinjie
Ye, Kai
Xu, Dan
Frequent exacerbators of chronic obstructive pulmonary disease have distinguishable sputum microbiome signatures during clinical stability
title Frequent exacerbators of chronic obstructive pulmonary disease have distinguishable sputum microbiome signatures during clinical stability
title_full Frequent exacerbators of chronic obstructive pulmonary disease have distinguishable sputum microbiome signatures during clinical stability
title_fullStr Frequent exacerbators of chronic obstructive pulmonary disease have distinguishable sputum microbiome signatures during clinical stability
title_full_unstemmed Frequent exacerbators of chronic obstructive pulmonary disease have distinguishable sputum microbiome signatures during clinical stability
title_short Frequent exacerbators of chronic obstructive pulmonary disease have distinguishable sputum microbiome signatures during clinical stability
title_sort frequent exacerbators of chronic obstructive pulmonary disease have distinguishable sputum microbiome signatures during clinical stability
topic Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9753979/
https://www.ncbi.nlm.nih.gov/pubmed/36532417
http://dx.doi.org/10.3389/fmicb.2022.1037037
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