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Internal and external microbiota of home-caught Anopheles coluzzii (Diptera: Culicidae) from Côte d’Ivoire, Africa: Mosquitoes are filthy

Over the past 10 years, studies using high-throughput 16S rRNA gene sequencing have shown that mosquitoes harbor diverse bacterial communities in their digestive system. However, no previous research has examined the total bacteria community inside versus outside of mosquitoes and whether bacteria f...

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Autores principales: Chen, Kaiying, Ponnusamy, Loganathan, Mouhamadou, Chouaïbou S., Fodjo, Behi Kouadio, Sadia, Gba Christabelle, Affoue, France Paraudie Kouadio, Deguenon, Jean M., Roe, R. Michael
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9754230/
https://www.ncbi.nlm.nih.gov/pubmed/36520830
http://dx.doi.org/10.1371/journal.pone.0278912
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author Chen, Kaiying
Ponnusamy, Loganathan
Mouhamadou, Chouaïbou S.
Fodjo, Behi Kouadio
Sadia, Gba Christabelle
Affoue, France Paraudie Kouadio
Deguenon, Jean M.
Roe, R. Michael
author_facet Chen, Kaiying
Ponnusamy, Loganathan
Mouhamadou, Chouaïbou S.
Fodjo, Behi Kouadio
Sadia, Gba Christabelle
Affoue, France Paraudie Kouadio
Deguenon, Jean M.
Roe, R. Michael
author_sort Chen, Kaiying
collection PubMed
description Over the past 10 years, studies using high-throughput 16S rRNA gene sequencing have shown that mosquitoes harbor diverse bacterial communities in their digestive system. However, no previous research has examined the total bacteria community inside versus outside of mosquitoes and whether bacteria found on the outside could represent a potential health threat through mechanical transfer. We examined the bacterial community of the external surface and internal body of female Anopheles coluzzii adults collected from homes in Côte d’Ivoire, Africa, by Illumina sequencing of the V3 to V4 region of 16S rRNA gene. Anopheles coluzzii is in the Anopheles gambiae sensu lato (s.l.) species complex and important in the transmission of malaria. The total 16S rRNA reads were assigned to 34 phyla, 73 orders, 325 families, and 700 genera. At the genus level, the most abundant genera inside and outside combined were Bacillus, Staphylococcus, Enterobacter, Corynebacterium, Kocuria, Providencia, and Sphingomonas. Mosquitoes had a greater diversity of bacterial taxa internally compared to the outside. The internal bacterial communities were similar between homes, while the external body samples were significantly different between homes. The bacteria on the external body were associated with plants, human and animal skin, and human and animal infections. Internally, Rickettsia bellii and Rickettsia typhi were found, potentially of importance, since this genus is associated with human diseases. Based on these findings, further research is warranted to assess the potential mechanical transmission of bacteria by mosquitoes moving into homes and the importance of the internal mosquito microbiota in human health.
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spelling pubmed-97542302022-12-16 Internal and external microbiota of home-caught Anopheles coluzzii (Diptera: Culicidae) from Côte d’Ivoire, Africa: Mosquitoes are filthy Chen, Kaiying Ponnusamy, Loganathan Mouhamadou, Chouaïbou S. Fodjo, Behi Kouadio Sadia, Gba Christabelle Affoue, France Paraudie Kouadio Deguenon, Jean M. Roe, R. Michael PLoS One Research Article Over the past 10 years, studies using high-throughput 16S rRNA gene sequencing have shown that mosquitoes harbor diverse bacterial communities in their digestive system. However, no previous research has examined the total bacteria community inside versus outside of mosquitoes and whether bacteria found on the outside could represent a potential health threat through mechanical transfer. We examined the bacterial community of the external surface and internal body of female Anopheles coluzzii adults collected from homes in Côte d’Ivoire, Africa, by Illumina sequencing of the V3 to V4 region of 16S rRNA gene. Anopheles coluzzii is in the Anopheles gambiae sensu lato (s.l.) species complex and important in the transmission of malaria. The total 16S rRNA reads were assigned to 34 phyla, 73 orders, 325 families, and 700 genera. At the genus level, the most abundant genera inside and outside combined were Bacillus, Staphylococcus, Enterobacter, Corynebacterium, Kocuria, Providencia, and Sphingomonas. Mosquitoes had a greater diversity of bacterial taxa internally compared to the outside. The internal bacterial communities were similar between homes, while the external body samples were significantly different between homes. The bacteria on the external body were associated with plants, human and animal skin, and human and animal infections. Internally, Rickettsia bellii and Rickettsia typhi were found, potentially of importance, since this genus is associated with human diseases. Based on these findings, further research is warranted to assess the potential mechanical transmission of bacteria by mosquitoes moving into homes and the importance of the internal mosquito microbiota in human health. Public Library of Science 2022-12-15 /pmc/articles/PMC9754230/ /pubmed/36520830 http://dx.doi.org/10.1371/journal.pone.0278912 Text en © 2022 Chen et al https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Chen, Kaiying
Ponnusamy, Loganathan
Mouhamadou, Chouaïbou S.
Fodjo, Behi Kouadio
Sadia, Gba Christabelle
Affoue, France Paraudie Kouadio
Deguenon, Jean M.
Roe, R. Michael
Internal and external microbiota of home-caught Anopheles coluzzii (Diptera: Culicidae) from Côte d’Ivoire, Africa: Mosquitoes are filthy
title Internal and external microbiota of home-caught Anopheles coluzzii (Diptera: Culicidae) from Côte d’Ivoire, Africa: Mosquitoes are filthy
title_full Internal and external microbiota of home-caught Anopheles coluzzii (Diptera: Culicidae) from Côte d’Ivoire, Africa: Mosquitoes are filthy
title_fullStr Internal and external microbiota of home-caught Anopheles coluzzii (Diptera: Culicidae) from Côte d’Ivoire, Africa: Mosquitoes are filthy
title_full_unstemmed Internal and external microbiota of home-caught Anopheles coluzzii (Diptera: Culicidae) from Côte d’Ivoire, Africa: Mosquitoes are filthy
title_short Internal and external microbiota of home-caught Anopheles coluzzii (Diptera: Culicidae) from Côte d’Ivoire, Africa: Mosquitoes are filthy
title_sort internal and external microbiota of home-caught anopheles coluzzii (diptera: culicidae) from côte d’ivoire, africa: mosquitoes are filthy
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9754230/
https://www.ncbi.nlm.nih.gov/pubmed/36520830
http://dx.doi.org/10.1371/journal.pone.0278912
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