Cargando…

Achieving single nucleotide sensitivity in direct hybridization genome imaging

Direct visualization of point mutations in situ can be informative for studying genetic diseases and nuclear biology. We describe a direct hybridization genome imaging method with single-nucleotide sensitivity, single guide genome oligopaint via local denaturation fluorescence in situ hybridization...

Descripción completa

Detalles Bibliográficos
Autores principales:	Wang, Yanbo, Cottle, W. Taylor, Wang, Haobo, Gavrilov, Momcilo, Zou, Roger S., Pham, Minh-Tam, Yegnasubramanian, Srinivasan, Bailey, Scott, Ha, Taekjip
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Nature Publishing Group UK 2022
Materias:	Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9755149/ https://www.ncbi.nlm.nih.gov/pubmed/36522352 http://dx.doi.org/10.1038/s41467-022-35476-y

Ejemplares similares

Catalytic DNA Polymerization Can Be Expedited by Active Product Release
por: Moerman, Pepijn G., et al.
Publicado: (2022)

Directing Uphill Strand Displacement with an Engineered Superhelicase
por: Hall-Thomsen, Helena, et al.
Publicado: (2023)

Single molecule analysis of effects of non-canonical guide RNAs and specificity-enhancing mutations on Cas9-induced DNA unwinding
por: Okafor, Ikenna C, et al.
Publicado: (2019)

Engineered helicase replaces thermocycler in DNA amplification while retaining desired PCR characteristics
por: Gavrilov, Momčilo, et al.
Publicado: (2022)

Improving the specificity of nucleic acid detection with endonuclease-actuated degradation
por: Zou, Roger S., et al.
Publicado: (2022)

E. coli Rep helicase and RecA recombinase unwind G4 DNA and are important for resistance to G4-stabilizing ligands
por: Paul, Tapas, et al.
Publicado: (2020)

Sequence-dependent mechanochemical coupling of helicase translocation and unwinding at single-nucleotide resolution
por: Laszlo, Andrew H., et al.
Publicado: (2022)

CRISPR deactivation in mammalian cells using photocleavable guide RNAs
por: Zou, Roger S., et al.
Publicado: (2021)

Prostate cancer epigenetics and its clinical implications
por: Yegnasubramanian, Srinivasan
Publicado: (2016)

BuGZ exhibits guanine nucleotide exchange factor activity toward tubulin
por: Yon, Wesley J., et al.
Publicado: (2023)

Combination of methylated-DNA precipitation and methylation-sensitive restriction enzymes (COMPARE-MS) for the rapid, sensitive and quantitative detection of DNA methylation
por: Yegnasubramanian, Srinivasan, et al.
Publicado: (2006)

Improving the sensitivity of in vivo CRISPR off-target detection with DISCOVER-Seq+
por: Zou, Roger S., et al.
Publicado: (2023)

Quantifying single nucleotide variant detection sensitivity in exome sequencing
por: Meynert, Alison M, et al.
Publicado: (2013)

Constructing modular and universal single molecule tension sensor using protein G to study mechano-sensitive receptors
por: Wang, Xuefeng, et al.
Publicado: (2016)

Lipoic Acid-Modified Oligoethyleneimine-Mediated miR-34a Delivery to Achieve the Anti-Tumor Efficacy
por: Huang, Yu, et al.
Publicado: (2021)

Hybrid LNP Prime Dendritic Cells for Nucleotide Delivery
por: Das, Riddha, et al.
Publicado: (2023)

RNA-Seq of the Nucleolus Reveals Abundant SNORD44-Derived Small RNAs
por: Bai, Baoyan, et al.
Publicado: (2014)

Global DNA methylation changes and differential gene expression in Anaplasma phagocytophilum-infected human neutrophils
por: Sinclair, Sara H. G., et al.
Publicado: (2015)

Computational estimation of potential inhibitors from known drugs against the main protease of SARS-CoV-2
por: Tam, Nguyen Minh, et al.
Publicado: (2021)

Cross Talk Between Cyclic Nucleotides and Calcium Signaling Pathways in Plants–Achievements and Prospects
por: Świeżawska-Boniecka, Brygida, et al.
Publicado: (2021)

Achieving Verifiable Decision Tree Prediction on Hybrid Blockchains
por: Fu, Moxuan, et al.
Publicado: (2023)

The light side of the force
por: Basu, Aakash, et al.
Publicado: (2016)

Nanomechanics and co-transcriptional folding of Spinach and Mango
por: Mitra, Jaba, et al.
Publicado: (2019)

Streamlining effects of extra telomeric repeat on telomeric DNA folding revealed by fluorescence-force spectroscopy
por: Mitra, Jaba, et al.
Publicado: (2019)

A Hybrid Bionic Image Sensor Achieving FOV Extension and Foveated Imaging
por: Hao, Qun, et al.
Publicado: (2018)

Stimuli-Responsive Nucleotide–Amino Acid Hybrid Supramolecular Hydrogels
por: Mulvee, Matthew, et al.
Publicado: (2021)

Proposing a hybrid metaheuristic optimization algorithm and machine learning model for energy use forecast in non-residential buildings
por: Ngo, Ngoc-Tri, et al.
Publicado: (2022)

Hexameric helicase G40P unwinds DNA in single base pair steps
por: Schlierf, Michael, et al.
Publicado: (2019)

Promoting global health transdisciplinary research for planetary health: Towards achieving the 2030 Agenda for Sustainable Development
por: Pham, Long Tam, et al.
Publicado: (2023)

Integrated single-cell and bulk gene expression and ATAC-seq reveals heterogeneity and early changes in pathways associated with resistance to cetuximab in HNSCC-sensitive cell lines
por: Kagohara, Luciane T., et al.
Publicado: (2020)

Identifying Phased Mutations and Complex Rearrangements in Human Prostate Cancer Cell Lines through Linked-Read Whole-Genome Sequencing
por: Pham, Minh-Tam, et al.
Publicado: (2022)

Single Molecule FRET Analysis of CRISPR Cas9 Single Guide RNA Folding Dynamics
por: Okafor, Ikenna C., et al.
Publicado: (2022)

Mind your tag in single-molecule measurements
por: Merino Urteaga, Raquel, et al.
Publicado: (2023)

Achieving Reliability in Cloud Computing by a Novel Hybrid Approach
por: Shahid, Muhammad Asim, et al.
Publicado: (2023)

Correction: Integrated single-cell and bulk gene expression and ATAC-seq reveals heterogeneity and early changes in pathways associated with resistance to cetuximab in HNSCC-sensitive cell lines
por: Kagohara, Luciane T., et al.
Publicado: (2020)

Sensitive assay for the detection of cyclic nucleotides by mass spectrometry
por: Spangler, Corinna M, et al.
Publicado: (2009)

Nucleotide biosynthesis links glutathione metabolism to ferroptosis sensitivity
por: Tarangelo, Amy, et al.
Publicado: (2022)

Disulfiram-loaded porous PLGA microparticle for inhibiting the proliferation and migration of non-small-cell lung cancer
por: Wang, Chenhui, et al.
Publicado: (2017)

Primary-tiller panicle number is critical to achieving high grain yields in machine-transplanted hybrid rice
por: Huang, Min, et al.
Publicado: (2020)

Phenolic Compounds and Antioxidant Activity of Phalaenopsis Orchid Hybrids
por: Minh, Truong Ngoc, et al.
Publicado: (2016)

Cannot write session to /tmp/vufind_sessions/sess_7lfpvc55cv24q53qs21m98smq5