Networked lymphatic endothelial cells in a transplanted cell sheet contribute to form functional lymphatic vessels

This study evaluated whether cell sheets containing a network of lymphatic endothelial cells (LECs) promoted lymphangiogenesis after transplantation in vivo. Cell sheets with a LEC network were constructed by co-culturing LECs and adipose-derived stem cells (ASCs) on temperature-responsive culture d...

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Autores principales: Nagahara, Ayumi Inoue, Homma, Jun, Ryu, Bikei, Sekine, Hidekazu, Higashi, Yuhei, Shimizu, Tatsuya, Kawamata, Takakazu
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2022
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9755306/
https://www.ncbi.nlm.nih.gov/pubmed/36522421
http://dx.doi.org/10.1038/s41598-022-26041-0
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author Nagahara, Ayumi Inoue
Homma, Jun
Ryu, Bikei
Sekine, Hidekazu
Higashi, Yuhei
Shimizu, Tatsuya
Kawamata, Takakazu
author_facet Nagahara, Ayumi Inoue
Homma, Jun
Ryu, Bikei
Sekine, Hidekazu
Higashi, Yuhei
Shimizu, Tatsuya
Kawamata, Takakazu
author_sort Nagahara, Ayumi Inoue
collection PubMed
description This study evaluated whether cell sheets containing a network of lymphatic endothelial cells (LECs) promoted lymphangiogenesis after transplantation in vivo. Cell sheets with a LEC network were constructed by co-culturing LECs and adipose-derived stem cells (ASCs) on temperature-responsive culture dishes. A cell ratio of 3:2 (vs. 1:4) generated networks with more branches and longer branch lengths. LEC-derived lymphatic vessels were observed 2 weeks after transplantation of a three-layered cell sheet construct onto rat gluteal muscle. Lymphatic vessel number, diameter and depth were greatest for a construct comprising two ASC sheets stacked on a LEC/ASC (3:2 ratio) sheet. Transplantation of this construct in a rat model of femoral lymphangiectomy led to the formation of functional lymphatic vessels containing both transplanted and host LECs. Further development of this technique may lead to a new method of promoting lymphangiogenesis.
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spelling pubmed-97553062022-12-17 Networked lymphatic endothelial cells in a transplanted cell sheet contribute to form functional lymphatic vessels Nagahara, Ayumi Inoue Homma, Jun Ryu, Bikei Sekine, Hidekazu Higashi, Yuhei Shimizu, Tatsuya Kawamata, Takakazu Sci Rep Article This study evaluated whether cell sheets containing a network of lymphatic endothelial cells (LECs) promoted lymphangiogenesis after transplantation in vivo. Cell sheets with a LEC network were constructed by co-culturing LECs and adipose-derived stem cells (ASCs) on temperature-responsive culture dishes. A cell ratio of 3:2 (vs. 1:4) generated networks with more branches and longer branch lengths. LEC-derived lymphatic vessels were observed 2 weeks after transplantation of a three-layered cell sheet construct onto rat gluteal muscle. Lymphatic vessel number, diameter and depth were greatest for a construct comprising two ASC sheets stacked on a LEC/ASC (3:2 ratio) sheet. Transplantation of this construct in a rat model of femoral lymphangiectomy led to the formation of functional lymphatic vessels containing both transplanted and host LECs. Further development of this technique may lead to a new method of promoting lymphangiogenesis. Nature Publishing Group UK 2022-12-15 /pmc/articles/PMC9755306/ /pubmed/36522421 http://dx.doi.org/10.1038/s41598-022-26041-0 Text en © The Author(s) 2022 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Article
Nagahara, Ayumi Inoue
Homma, Jun
Ryu, Bikei
Sekine, Hidekazu
Higashi, Yuhei
Shimizu, Tatsuya
Kawamata, Takakazu
Networked lymphatic endothelial cells in a transplanted cell sheet contribute to form functional lymphatic vessels
title Networked lymphatic endothelial cells in a transplanted cell sheet contribute to form functional lymphatic vessels
title_full Networked lymphatic endothelial cells in a transplanted cell sheet contribute to form functional lymphatic vessels
title_fullStr Networked lymphatic endothelial cells in a transplanted cell sheet contribute to form functional lymphatic vessels
title_full_unstemmed Networked lymphatic endothelial cells in a transplanted cell sheet contribute to form functional lymphatic vessels
title_short Networked lymphatic endothelial cells in a transplanted cell sheet contribute to form functional lymphatic vessels
title_sort networked lymphatic endothelial cells in a transplanted cell sheet contribute to form functional lymphatic vessels
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9755306/
https://www.ncbi.nlm.nih.gov/pubmed/36522421
http://dx.doi.org/10.1038/s41598-022-26041-0
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