Cargando…

“Going with the flow” in modeling fibrinolysis

The formation of thrombi is shaped by intravascular shear stress, influencing both fibrin architecture and the cellular composition which has downstream implications in terms of stability against mechanical and fibrinolytic forces. There have been many advancements in the development of models that...

Descripción completa

Detalles Bibliográficos
Autores principales: Whyte, Claire S., Mutch, Nicola J.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9755328/
https://www.ncbi.nlm.nih.gov/pubmed/36531720
http://dx.doi.org/10.3389/fcvm.2022.1054541
_version_ 1784851407403220992
author Whyte, Claire S.
Mutch, Nicola J.
author_facet Whyte, Claire S.
Mutch, Nicola J.
author_sort Whyte, Claire S.
collection PubMed
description The formation of thrombi is shaped by intravascular shear stress, influencing both fibrin architecture and the cellular composition which has downstream implications in terms of stability against mechanical and fibrinolytic forces. There have been many advancements in the development of models that incorporate flow rates akin to those found in vivo. Both thrombus formation and breakdown are simultaneous processes, the balance of which dictates the size, persistence and resolution of thrombi. Therefore, there is a requirement to have models which mimic the physiological shear experienced within the vasculature which in turn influences the fibrinolytic degradation of the thrombus. Here, we discuss various assays for fibrinolysis and importantly the development of novel models that incorporate physiological shear rates. These models are essential tools to untangle the molecular and cellular processes which govern fibrinolysis and can recreate the conditions within normal and diseased vessels to determine how these processes become perturbed in a pathophysiological setting. They also have utility to assess novel drug targets and antithrombotic drugs that influence thrombus stability.
format Online
Article
Text
id pubmed-9755328
institution National Center for Biotechnology Information
language English
publishDate 2022
publisher Frontiers Media S.A.
record_format MEDLINE/PubMed
spelling pubmed-97553282022-12-17 “Going with the flow” in modeling fibrinolysis Whyte, Claire S. Mutch, Nicola J. Front Cardiovasc Med Cardiovascular Medicine The formation of thrombi is shaped by intravascular shear stress, influencing both fibrin architecture and the cellular composition which has downstream implications in terms of stability against mechanical and fibrinolytic forces. There have been many advancements in the development of models that incorporate flow rates akin to those found in vivo. Both thrombus formation and breakdown are simultaneous processes, the balance of which dictates the size, persistence and resolution of thrombi. Therefore, there is a requirement to have models which mimic the physiological shear experienced within the vasculature which in turn influences the fibrinolytic degradation of the thrombus. Here, we discuss various assays for fibrinolysis and importantly the development of novel models that incorporate physiological shear rates. These models are essential tools to untangle the molecular and cellular processes which govern fibrinolysis and can recreate the conditions within normal and diseased vessels to determine how these processes become perturbed in a pathophysiological setting. They also have utility to assess novel drug targets and antithrombotic drugs that influence thrombus stability. Frontiers Media S.A. 2022-12-02 /pmc/articles/PMC9755328/ /pubmed/36531720 http://dx.doi.org/10.3389/fcvm.2022.1054541 Text en Copyright © 2022 Whyte and Mutch. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Cardiovascular Medicine
Whyte, Claire S.
Mutch, Nicola J.
“Going with the flow” in modeling fibrinolysis
title “Going with the flow” in modeling fibrinolysis
title_full “Going with the flow” in modeling fibrinolysis
title_fullStr “Going with the flow” in modeling fibrinolysis
title_full_unstemmed “Going with the flow” in modeling fibrinolysis
title_short “Going with the flow” in modeling fibrinolysis
title_sort “going with the flow” in modeling fibrinolysis
topic Cardiovascular Medicine
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9755328/
https://www.ncbi.nlm.nih.gov/pubmed/36531720
http://dx.doi.org/10.3389/fcvm.2022.1054541
work_keys_str_mv AT whyteclaires goingwiththeflowinmodelingfibrinolysis
AT mutchnicolaj goingwiththeflowinmodelingfibrinolysis