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Improving berry quality and antioxidant ability in ‘Ruidu Hongyu’ grapevine through preharvest exogenous 2,4-epibrassinolide, jasmonic acid and their signaling inhibitors by regulating endogenous phytohormones

Grape berries contain a variety of metabolites, such as anthocyanins, sugars, fatty acids, and antioxidants. Endogenous phytohormones strongly influence these metabolites, which regulate berry quality improvement. In this study, we evaluated the effects of 2,4-epibrassinolide (EBR, brassinolide (BR)...

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Autores principales: Li, Jiajia, Javed, Hafiz Umer, Wu, Zishu, Wang, Lei, Han, Jiayu, Zhang, Ying, Ma, Chao, Jiu, Songtao, Zhang, Caixi, Wang, Shiping
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9755660/
https://www.ncbi.nlm.nih.gov/pubmed/36531411
http://dx.doi.org/10.3389/fpls.2022.1035022
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author Li, Jiajia
Javed, Hafiz Umer
Wu, Zishu
Wang, Lei
Han, Jiayu
Zhang, Ying
Ma, Chao
Jiu, Songtao
Zhang, Caixi
Wang, Shiping
author_facet Li, Jiajia
Javed, Hafiz Umer
Wu, Zishu
Wang, Lei
Han, Jiayu
Zhang, Ying
Ma, Chao
Jiu, Songtao
Zhang, Caixi
Wang, Shiping
author_sort Li, Jiajia
collection PubMed
description Grape berries contain a variety of metabolites, such as anthocyanins, sugars, fatty acids, and antioxidants. Endogenous phytohormones strongly influence these metabolites, which regulate berry quality improvement. In this study, we evaluated the effects of 2,4-epibrassinolide (EBR, brassinolide (BR)-like growth regulator), jasmonic acid (JA), and their signaling inhibitors brassinazole (Brz), and sodium diethyldithiocarbamate (DIECA) on berry quality and antioxidant ability. Overall, the pre-harvest application of 0.5 mg L(-1) EBR and 100 μmol L(-1) JA significantly influences the quality of the grape berry. Results showed that EBR was superior to other treatments at enhancing the content of different metabolites, including anthocyanins, fructose, glucose, and a variety of fatty acids, in grapes. EBR and JA also enhanced the synthesis of gibberellin(3) (GA(3)), cytokinin (CTK), salicylic acid (SA), JA, methyl jasmonate (MeJA), BR, and abscisic acid (ABA), while inhibiting the synthesis of auxin (IAA). Most genes related to BR/JA and anthocyanins/sugars/fatty acids biosynthesis were up-regulated. The effects of Brz and DIECA on the grape berry quality were totally reversed throughout the study, as shown by EBR and JA. According to correlation analysis, EBR and JA have a beneficial positive interaction that promotes the formation of strong coherences in grape berries between ABA/IAA/ZT-fruit expansion, BR/JA/MeJA/GA(3)/ZR-biochemical characteristics development, JA/MeJA/ABA/GA(3)/SA/ZR-antioxidant capacity enhancement, and JA/MeJA/IAA/GA(3)/ZT/ZR-fatty acids accumulation. In this regard, we concluded that preharvest exogenous 0.5 mg L(-1) EBR and 100 μmol L(-1) JA is a successful way to improve grape berry quality.
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spelling pubmed-97556602022-12-17 Improving berry quality and antioxidant ability in ‘Ruidu Hongyu’ grapevine through preharvest exogenous 2,4-epibrassinolide, jasmonic acid and their signaling inhibitors by regulating endogenous phytohormones Li, Jiajia Javed, Hafiz Umer Wu, Zishu Wang, Lei Han, Jiayu Zhang, Ying Ma, Chao Jiu, Songtao Zhang, Caixi Wang, Shiping Front Plant Sci Plant Science Grape berries contain a variety of metabolites, such as anthocyanins, sugars, fatty acids, and antioxidants. Endogenous phytohormones strongly influence these metabolites, which regulate berry quality improvement. In this study, we evaluated the effects of 2,4-epibrassinolide (EBR, brassinolide (BR)-like growth regulator), jasmonic acid (JA), and their signaling inhibitors brassinazole (Brz), and sodium diethyldithiocarbamate (DIECA) on berry quality and antioxidant ability. Overall, the pre-harvest application of 0.5 mg L(-1) EBR and 100 μmol L(-1) JA significantly influences the quality of the grape berry. Results showed that EBR was superior to other treatments at enhancing the content of different metabolites, including anthocyanins, fructose, glucose, and a variety of fatty acids, in grapes. EBR and JA also enhanced the synthesis of gibberellin(3) (GA(3)), cytokinin (CTK), salicylic acid (SA), JA, methyl jasmonate (MeJA), BR, and abscisic acid (ABA), while inhibiting the synthesis of auxin (IAA). Most genes related to BR/JA and anthocyanins/sugars/fatty acids biosynthesis were up-regulated. The effects of Brz and DIECA on the grape berry quality were totally reversed throughout the study, as shown by EBR and JA. According to correlation analysis, EBR and JA have a beneficial positive interaction that promotes the formation of strong coherences in grape berries between ABA/IAA/ZT-fruit expansion, BR/JA/MeJA/GA(3)/ZR-biochemical characteristics development, JA/MeJA/ABA/GA(3)/SA/ZR-antioxidant capacity enhancement, and JA/MeJA/IAA/GA(3)/ZT/ZR-fatty acids accumulation. In this regard, we concluded that preharvest exogenous 0.5 mg L(-1) EBR and 100 μmol L(-1) JA is a successful way to improve grape berry quality. Frontiers Media S.A. 2022-12-02 /pmc/articles/PMC9755660/ /pubmed/36531411 http://dx.doi.org/10.3389/fpls.2022.1035022 Text en Copyright © 2022 Li, Javed, Wu, Wang, Han, Zhang, Ma, Jiu, Zhang and Wang https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Plant Science
Li, Jiajia
Javed, Hafiz Umer
Wu, Zishu
Wang, Lei
Han, Jiayu
Zhang, Ying
Ma, Chao
Jiu, Songtao
Zhang, Caixi
Wang, Shiping
Improving berry quality and antioxidant ability in ‘Ruidu Hongyu’ grapevine through preharvest exogenous 2,4-epibrassinolide, jasmonic acid and their signaling inhibitors by regulating endogenous phytohormones
title Improving berry quality and antioxidant ability in ‘Ruidu Hongyu’ grapevine through preharvest exogenous 2,4-epibrassinolide, jasmonic acid and their signaling inhibitors by regulating endogenous phytohormones
title_full Improving berry quality and antioxidant ability in ‘Ruidu Hongyu’ grapevine through preharvest exogenous 2,4-epibrassinolide, jasmonic acid and their signaling inhibitors by regulating endogenous phytohormones
title_fullStr Improving berry quality and antioxidant ability in ‘Ruidu Hongyu’ grapevine through preharvest exogenous 2,4-epibrassinolide, jasmonic acid and their signaling inhibitors by regulating endogenous phytohormones
title_full_unstemmed Improving berry quality and antioxidant ability in ‘Ruidu Hongyu’ grapevine through preharvest exogenous 2,4-epibrassinolide, jasmonic acid and their signaling inhibitors by regulating endogenous phytohormones
title_short Improving berry quality and antioxidant ability in ‘Ruidu Hongyu’ grapevine through preharvest exogenous 2,4-epibrassinolide, jasmonic acid and their signaling inhibitors by regulating endogenous phytohormones
title_sort improving berry quality and antioxidant ability in ‘ruidu hongyu’ grapevine through preharvest exogenous 2,4-epibrassinolide, jasmonic acid and their signaling inhibitors by regulating endogenous phytohormones
topic Plant Science
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9755660/
https://www.ncbi.nlm.nih.gov/pubmed/36531411
http://dx.doi.org/10.3389/fpls.2022.1035022
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