Cargando…
The Protein Unfolded State: One, No One and One Hundred Thousand
[Image: see text] Many in vitro studies, in which proteins have been unfolded by the action of a variety of physical or chemical agents, have led to the definition of a folded versus an unfolded state and to the question of what is the nature of the unfolded state. The unstructured nature of this st...
Autores principales: | , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
American Chemical Society
2022
|
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9756289/ https://www.ncbi.nlm.nih.gov/pubmed/36450361 http://dx.doi.org/10.1021/jacs.2c07696 |
_version_ | 1784851601493590016 |
---|---|
author | Pastore, Annalisa Temussi, Piero Andrea |
author_facet | Pastore, Annalisa Temussi, Piero Andrea |
author_sort | Pastore, Annalisa |
collection | PubMed |
description | [Image: see text] Many in vitro studies, in which proteins have been unfolded by the action of a variety of physical or chemical agents, have led to the definition of a folded versus an unfolded state and to the question of what is the nature of the unfolded state. The unstructured nature of this state could suggest that “the” unfolded state is a unique entity which holds true for all kinds of unfolding processes. This assumption has to be questioned because the unfolding processes under different stress conditions are dictated by entirely different mechanisms. As a consequence, it can be easily understood that the final state, generically referred to as “the unfolded state”, can be completely different for each of the unfolding processes. The present review examines recent data on the characteristics of the unfolded states emerging from experiments under different conditions, focusing specific attention to the level of compaction of the unfolded species. |
format | Online Article Text |
id | pubmed-9756289 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | American Chemical Society |
record_format | MEDLINE/PubMed |
spelling | pubmed-97562892022-12-17 The Protein Unfolded State: One, No One and One Hundred Thousand Pastore, Annalisa Temussi, Piero Andrea J Am Chem Soc [Image: see text] Many in vitro studies, in which proteins have been unfolded by the action of a variety of physical or chemical agents, have led to the definition of a folded versus an unfolded state and to the question of what is the nature of the unfolded state. The unstructured nature of this state could suggest that “the” unfolded state is a unique entity which holds true for all kinds of unfolding processes. This assumption has to be questioned because the unfolding processes under different stress conditions are dictated by entirely different mechanisms. As a consequence, it can be easily understood that the final state, generically referred to as “the unfolded state”, can be completely different for each of the unfolding processes. The present review examines recent data on the characteristics of the unfolded states emerging from experiments under different conditions, focusing specific attention to the level of compaction of the unfolded species. American Chemical Society 2022-11-30 2022-12-14 /pmc/articles/PMC9756289/ /pubmed/36450361 http://dx.doi.org/10.1021/jacs.2c07696 Text en © 2022 The Authors. Published by American Chemical Society https://creativecommons.org/licenses/by-nc-nd/4.0/Permits non-commercial access and re-use, provided that author attribution and integrity are maintained; but does not permit creation of adaptations or other derivative works (https://creativecommons.org/licenses/by-nc-nd/4.0/). |
spellingShingle | Pastore, Annalisa Temussi, Piero Andrea The Protein Unfolded State: One, No One and One Hundred Thousand |
title | The Protein Unfolded
State: One, No One and One Hundred
Thousand |
title_full | The Protein Unfolded
State: One, No One and One Hundred
Thousand |
title_fullStr | The Protein Unfolded
State: One, No One and One Hundred
Thousand |
title_full_unstemmed | The Protein Unfolded
State: One, No One and One Hundred
Thousand |
title_short | The Protein Unfolded
State: One, No One and One Hundred
Thousand |
title_sort | protein unfolded
state: one, no one and one hundred
thousand |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9756289/ https://www.ncbi.nlm.nih.gov/pubmed/36450361 http://dx.doi.org/10.1021/jacs.2c07696 |
work_keys_str_mv | AT pastoreannalisa theproteinunfoldedstateonenooneandonehundredthousand AT temussipieroandrea theproteinunfoldedstateonenooneandonehundredthousand AT pastoreannalisa proteinunfoldedstateonenooneandonehundredthousand AT temussipieroandrea proteinunfoldedstateonenooneandonehundredthousand |