Cargando…
Monitor Tumor pHe and Response Longitudinally during Treatment Using CEST MRI-Detectable Alginate Microbeads
[Image: see text] Imaging pHe of the tumor microenvironment has paramount importance for characterizing aggressive, invasive tumors, as well as therapeutic responses. Here, a robust approach to image pH changes in the tumor microenvironment longitudinally and during sodium bicarbonate treatment was...
Autores principales: | , , , , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
American Chemical Society
2022
|
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9756293/ https://www.ncbi.nlm.nih.gov/pubmed/36448714 http://dx.doi.org/10.1021/acsami.2c10493 |
_version_ | 1784851602442551296 |
---|---|
author | Xiao, Peng Huang, Jianpan Han, Xiongqi Cheu, Jacinth W. S. Liu, Yang Law, Lok Hin Lai, Joseph H. C. Li, Jiyu Park, Se Weon Wong, Carmen C. L. Lam, Raymond H. W. Chan, Kannie W. Y. |
author_facet | Xiao, Peng Huang, Jianpan Han, Xiongqi Cheu, Jacinth W. S. Liu, Yang Law, Lok Hin Lai, Joseph H. C. Li, Jiyu Park, Se Weon Wong, Carmen C. L. Lam, Raymond H. W. Chan, Kannie W. Y. |
author_sort | Xiao, Peng |
collection | PubMed |
description | [Image: see text] Imaging pHe of the tumor microenvironment has paramount importance for characterizing aggressive, invasive tumors, as well as therapeutic responses. Here, a robust approach to image pH changes in the tumor microenvironment longitudinally and during sodium bicarbonate treatment was reported. The pH-sensing microbeads were designed and prepared based on materials approved for clinical use, i.e., alginate microbead-containing computed tomography (CT) contrast-agent (iopamidol)-loaded liposomes (Iop-lipobeads). This Iop-lipobead prepared using a customized microfluidic device generated a CEST contrast of 10.6% at 4.2 ppm at pH 7.0, which was stable for 20 days in vitro. The CEST contrast decreased by 11.8% when the pH decreased from 7.0 to 6.5 in vitro. Optimized Iop-lipobeads next to tumors showed a significant increase of 19.7 ± 6.1% (p < 0.01) in CEST contrast at 4.2 ppm during the first 3 days of treatment and decreased to 15.2 ± 4.8% when treatment stopped. Notably, percentage changes in Iop-lipobeads were higher than that of amide CEST (11.7% and 9.1%) in tumors during and after treatment. These findings demonstrated that the Iop-lipobead could provide an independent and sensitive assessment of the pHe changes for a noninvasive and longitudinal monitoring of the treatment effects using multiple CEST contrast. |
format | Online Article Text |
id | pubmed-9756293 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | American Chemical Society |
record_format | MEDLINE/PubMed |
spelling | pubmed-97562932022-12-17 Monitor Tumor pHe and Response Longitudinally during Treatment Using CEST MRI-Detectable Alginate Microbeads Xiao, Peng Huang, Jianpan Han, Xiongqi Cheu, Jacinth W. S. Liu, Yang Law, Lok Hin Lai, Joseph H. C. Li, Jiyu Park, Se Weon Wong, Carmen C. L. Lam, Raymond H. W. Chan, Kannie W. Y. ACS Appl Mater Interfaces [Image: see text] Imaging pHe of the tumor microenvironment has paramount importance for characterizing aggressive, invasive tumors, as well as therapeutic responses. Here, a robust approach to image pH changes in the tumor microenvironment longitudinally and during sodium bicarbonate treatment was reported. The pH-sensing microbeads were designed and prepared based on materials approved for clinical use, i.e., alginate microbead-containing computed tomography (CT) contrast-agent (iopamidol)-loaded liposomes (Iop-lipobeads). This Iop-lipobead prepared using a customized microfluidic device generated a CEST contrast of 10.6% at 4.2 ppm at pH 7.0, which was stable for 20 days in vitro. The CEST contrast decreased by 11.8% when the pH decreased from 7.0 to 6.5 in vitro. Optimized Iop-lipobeads next to tumors showed a significant increase of 19.7 ± 6.1% (p < 0.01) in CEST contrast at 4.2 ppm during the first 3 days of treatment and decreased to 15.2 ± 4.8% when treatment stopped. Notably, percentage changes in Iop-lipobeads were higher than that of amide CEST (11.7% and 9.1%) in tumors during and after treatment. These findings demonstrated that the Iop-lipobead could provide an independent and sensitive assessment of the pHe changes for a noninvasive and longitudinal monitoring of the treatment effects using multiple CEST contrast. American Chemical Society 2022-11-30 2022-12-14 /pmc/articles/PMC9756293/ /pubmed/36448714 http://dx.doi.org/10.1021/acsami.2c10493 Text en © 2022 The Authors. Published by American Chemical Society https://creativecommons.org/licenses/by-nc-nd/4.0/Permits non-commercial access and re-use, provided that author attribution and integrity are maintained; but does not permit creation of adaptations or other derivative works (https://creativecommons.org/licenses/by-nc-nd/4.0/). |
spellingShingle | Xiao, Peng Huang, Jianpan Han, Xiongqi Cheu, Jacinth W. S. Liu, Yang Law, Lok Hin Lai, Joseph H. C. Li, Jiyu Park, Se Weon Wong, Carmen C. L. Lam, Raymond H. W. Chan, Kannie W. Y. Monitor Tumor pHe and Response Longitudinally during Treatment Using CEST MRI-Detectable Alginate Microbeads |
title | Monitor
Tumor pHe and Response Longitudinally during
Treatment Using CEST MRI-Detectable Alginate Microbeads |
title_full | Monitor
Tumor pHe and Response Longitudinally during
Treatment Using CEST MRI-Detectable Alginate Microbeads |
title_fullStr | Monitor
Tumor pHe and Response Longitudinally during
Treatment Using CEST MRI-Detectable Alginate Microbeads |
title_full_unstemmed | Monitor
Tumor pHe and Response Longitudinally during
Treatment Using CEST MRI-Detectable Alginate Microbeads |
title_short | Monitor
Tumor pHe and Response Longitudinally during
Treatment Using CEST MRI-Detectable Alginate Microbeads |
title_sort | monitor
tumor phe and response longitudinally during
treatment using cest mri-detectable alginate microbeads |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9756293/ https://www.ncbi.nlm.nih.gov/pubmed/36448714 http://dx.doi.org/10.1021/acsami.2c10493 |
work_keys_str_mv | AT xiaopeng monitortumorpheandresponselongitudinallyduringtreatmentusingcestmridetectablealginatemicrobeads AT huangjianpan monitortumorpheandresponselongitudinallyduringtreatmentusingcestmridetectablealginatemicrobeads AT hanxiongqi monitortumorpheandresponselongitudinallyduringtreatmentusingcestmridetectablealginatemicrobeads AT cheujacinthws monitortumorpheandresponselongitudinallyduringtreatmentusingcestmridetectablealginatemicrobeads AT liuyang monitortumorpheandresponselongitudinallyduringtreatmentusingcestmridetectablealginatemicrobeads AT lawlokhin monitortumorpheandresponselongitudinallyduringtreatmentusingcestmridetectablealginatemicrobeads AT laijosephhc monitortumorpheandresponselongitudinallyduringtreatmentusingcestmridetectablealginatemicrobeads AT lijiyu monitortumorpheandresponselongitudinallyduringtreatmentusingcestmridetectablealginatemicrobeads AT parkseweon monitortumorpheandresponselongitudinallyduringtreatmentusingcestmridetectablealginatemicrobeads AT wongcarmencl monitortumorpheandresponselongitudinallyduringtreatmentusingcestmridetectablealginatemicrobeads AT lamraymondhw monitortumorpheandresponselongitudinallyduringtreatmentusingcestmridetectablealginatemicrobeads AT chankanniewy monitortumorpheandresponselongitudinallyduringtreatmentusingcestmridetectablealginatemicrobeads |