Cargando…

Monitor Tumor pHe and Response Longitudinally during Treatment Using CEST MRI-Detectable Alginate Microbeads

[Image: see text] Imaging pHe of the tumor microenvironment has paramount importance for characterizing aggressive, invasive tumors, as well as therapeutic responses. Here, a robust approach to image pH changes in the tumor microenvironment longitudinally and during sodium bicarbonate treatment was...

Descripción completa

Detalles Bibliográficos
Autores principales: Xiao, Peng, Huang, Jianpan, Han, Xiongqi, Cheu, Jacinth W. S., Liu, Yang, Law, Lok Hin, Lai, Joseph H. C., Li, Jiyu, Park, Se Weon, Wong, Carmen C. L., Lam, Raymond H. W., Chan, Kannie W. Y.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Chemical Society 2022
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9756293/
https://www.ncbi.nlm.nih.gov/pubmed/36448714
http://dx.doi.org/10.1021/acsami.2c10493
_version_ 1784851602442551296
author Xiao, Peng
Huang, Jianpan
Han, Xiongqi
Cheu, Jacinth W. S.
Liu, Yang
Law, Lok Hin
Lai, Joseph H. C.
Li, Jiyu
Park, Se Weon
Wong, Carmen C. L.
Lam, Raymond H. W.
Chan, Kannie W. Y.
author_facet Xiao, Peng
Huang, Jianpan
Han, Xiongqi
Cheu, Jacinth W. S.
Liu, Yang
Law, Lok Hin
Lai, Joseph H. C.
Li, Jiyu
Park, Se Weon
Wong, Carmen C. L.
Lam, Raymond H. W.
Chan, Kannie W. Y.
author_sort Xiao, Peng
collection PubMed
description [Image: see text] Imaging pHe of the tumor microenvironment has paramount importance for characterizing aggressive, invasive tumors, as well as therapeutic responses. Here, a robust approach to image pH changes in the tumor microenvironment longitudinally and during sodium bicarbonate treatment was reported. The pH-sensing microbeads were designed and prepared based on materials approved for clinical use, i.e., alginate microbead-containing computed tomography (CT) contrast-agent (iopamidol)-loaded liposomes (Iop-lipobeads). This Iop-lipobead prepared using a customized microfluidic device generated a CEST contrast of 10.6% at 4.2 ppm at pH 7.0, which was stable for 20 days in vitro. The CEST contrast decreased by 11.8% when the pH decreased from 7.0 to 6.5 in vitro. Optimized Iop-lipobeads next to tumors showed a significant increase of 19.7 ± 6.1% (p < 0.01) in CEST contrast at 4.2 ppm during the first 3 days of treatment and decreased to 15.2 ± 4.8% when treatment stopped. Notably, percentage changes in Iop-lipobeads were higher than that of amide CEST (11.7% and 9.1%) in tumors during and after treatment. These findings demonstrated that the Iop-lipobead could provide an independent and sensitive assessment of the pHe changes for a noninvasive and longitudinal monitoring of the treatment effects using multiple CEST contrast.
format Online
Article
Text
id pubmed-9756293
institution National Center for Biotechnology Information
language English
publishDate 2022
publisher American Chemical Society
record_format MEDLINE/PubMed
spelling pubmed-97562932022-12-17 Monitor Tumor pHe and Response Longitudinally during Treatment Using CEST MRI-Detectable Alginate Microbeads Xiao, Peng Huang, Jianpan Han, Xiongqi Cheu, Jacinth W. S. Liu, Yang Law, Lok Hin Lai, Joseph H. C. Li, Jiyu Park, Se Weon Wong, Carmen C. L. Lam, Raymond H. W. Chan, Kannie W. Y. ACS Appl Mater Interfaces [Image: see text] Imaging pHe of the tumor microenvironment has paramount importance for characterizing aggressive, invasive tumors, as well as therapeutic responses. Here, a robust approach to image pH changes in the tumor microenvironment longitudinally and during sodium bicarbonate treatment was reported. The pH-sensing microbeads were designed and prepared based on materials approved for clinical use, i.e., alginate microbead-containing computed tomography (CT) contrast-agent (iopamidol)-loaded liposomes (Iop-lipobeads). This Iop-lipobead prepared using a customized microfluidic device generated a CEST contrast of 10.6% at 4.2 ppm at pH 7.0, which was stable for 20 days in vitro. The CEST contrast decreased by 11.8% when the pH decreased from 7.0 to 6.5 in vitro. Optimized Iop-lipobeads next to tumors showed a significant increase of 19.7 ± 6.1% (p < 0.01) in CEST contrast at 4.2 ppm during the first 3 days of treatment and decreased to 15.2 ± 4.8% when treatment stopped. Notably, percentage changes in Iop-lipobeads were higher than that of amide CEST (11.7% and 9.1%) in tumors during and after treatment. These findings demonstrated that the Iop-lipobead could provide an independent and sensitive assessment of the pHe changes for a noninvasive and longitudinal monitoring of the treatment effects using multiple CEST contrast. American Chemical Society 2022-11-30 2022-12-14 /pmc/articles/PMC9756293/ /pubmed/36448714 http://dx.doi.org/10.1021/acsami.2c10493 Text en © 2022 The Authors. Published by American Chemical Society https://creativecommons.org/licenses/by-nc-nd/4.0/Permits non-commercial access and re-use, provided that author attribution and integrity are maintained; but does not permit creation of adaptations or other derivative works (https://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Xiao, Peng
Huang, Jianpan
Han, Xiongqi
Cheu, Jacinth W. S.
Liu, Yang
Law, Lok Hin
Lai, Joseph H. C.
Li, Jiyu
Park, Se Weon
Wong, Carmen C. L.
Lam, Raymond H. W.
Chan, Kannie W. Y.
Monitor Tumor pHe and Response Longitudinally during Treatment Using CEST MRI-Detectable Alginate Microbeads
title Monitor Tumor pHe and Response Longitudinally during Treatment Using CEST MRI-Detectable Alginate Microbeads
title_full Monitor Tumor pHe and Response Longitudinally during Treatment Using CEST MRI-Detectable Alginate Microbeads
title_fullStr Monitor Tumor pHe and Response Longitudinally during Treatment Using CEST MRI-Detectable Alginate Microbeads
title_full_unstemmed Monitor Tumor pHe and Response Longitudinally during Treatment Using CEST MRI-Detectable Alginate Microbeads
title_short Monitor Tumor pHe and Response Longitudinally during Treatment Using CEST MRI-Detectable Alginate Microbeads
title_sort monitor tumor phe and response longitudinally during treatment using cest mri-detectable alginate microbeads
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9756293/
https://www.ncbi.nlm.nih.gov/pubmed/36448714
http://dx.doi.org/10.1021/acsami.2c10493
work_keys_str_mv AT xiaopeng monitortumorpheandresponselongitudinallyduringtreatmentusingcestmridetectablealginatemicrobeads
AT huangjianpan monitortumorpheandresponselongitudinallyduringtreatmentusingcestmridetectablealginatemicrobeads
AT hanxiongqi monitortumorpheandresponselongitudinallyduringtreatmentusingcestmridetectablealginatemicrobeads
AT cheujacinthws monitortumorpheandresponselongitudinallyduringtreatmentusingcestmridetectablealginatemicrobeads
AT liuyang monitortumorpheandresponselongitudinallyduringtreatmentusingcestmridetectablealginatemicrobeads
AT lawlokhin monitortumorpheandresponselongitudinallyduringtreatmentusingcestmridetectablealginatemicrobeads
AT laijosephhc monitortumorpheandresponselongitudinallyduringtreatmentusingcestmridetectablealginatemicrobeads
AT lijiyu monitortumorpheandresponselongitudinallyduringtreatmentusingcestmridetectablealginatemicrobeads
AT parkseweon monitortumorpheandresponselongitudinallyduringtreatmentusingcestmridetectablealginatemicrobeads
AT wongcarmencl monitortumorpheandresponselongitudinallyduringtreatmentusingcestmridetectablealginatemicrobeads
AT lamraymondhw monitortumorpheandresponselongitudinallyduringtreatmentusingcestmridetectablealginatemicrobeads
AT chankanniewy monitortumorpheandresponselongitudinallyduringtreatmentusingcestmridetectablealginatemicrobeads