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Metabolic engineering of the carotenoid biosynthetic pathway toward a specific and sensitive inorganic mercury biosensor

The toxicity of mercury (Hg) mainly depends on its form. Whole-cell biosensors respond selectively to toxic Hg(ii), efficiently transformed by environmental microbes into methylmercury, a highly toxic form that builds up in aquatic animals. Metabolically engineered Escherichia coli (E. coli) have su...

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Autores principales: Hui, Chang-ye, Hu, Shun-yu, Li, Li-mei, Yun, Jian-pei, Zhang, Yan-fang, Yi, Juan, Zhang, Nai-xing, Guo, Yan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Royal Society of Chemistry 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9756418/
https://www.ncbi.nlm.nih.gov/pubmed/36545109
http://dx.doi.org/10.1039/d2ra06764a
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author Hui, Chang-ye
Hu, Shun-yu
Li, Li-mei
Yun, Jian-pei
Zhang, Yan-fang
Yi, Juan
Zhang, Nai-xing
Guo, Yan
author_facet Hui, Chang-ye
Hu, Shun-yu
Li, Li-mei
Yun, Jian-pei
Zhang, Yan-fang
Yi, Juan
Zhang, Nai-xing
Guo, Yan
author_sort Hui, Chang-ye
collection PubMed
description The toxicity of mercury (Hg) mainly depends on its form. Whole-cell biosensors respond selectively to toxic Hg(ii), efficiently transformed by environmental microbes into methylmercury, a highly toxic form that builds up in aquatic animals. Metabolically engineered Escherichia coli (E. coli) have successfully produced rainbow colorants. By de novo reconstruction of the carotenoid synthetic pathway, the Hg(ii)-responsive production of lycopene and β-carotene enabled programmed E. coli to potentially become an optical biosensor for the qualitative and quantitative detection of ecotoxic Hg(ii). The red color of the lycopene-based biosensor cell pellet was visible upon exposure to 49 nM Hg(ii) and above. The orange β-carotene-based biosensor responded to a simple colorimetric assay as low as 12 nM Hg(ii). A linear response was observed at Hg(ii) concentrations ranging from 12 to 195 nM. Importantly, high specificity and good anti-interference capability suggested that metabolic engineering of the carotenoid biosynthesis was an alternative to developing a visual platform for the rapid analysis of the concentration and toxicity of Hg(ii) in environmentally polluted water.
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spelling pubmed-97564182022-12-20 Metabolic engineering of the carotenoid biosynthetic pathway toward a specific and sensitive inorganic mercury biosensor Hui, Chang-ye Hu, Shun-yu Li, Li-mei Yun, Jian-pei Zhang, Yan-fang Yi, Juan Zhang, Nai-xing Guo, Yan RSC Adv Chemistry The toxicity of mercury (Hg) mainly depends on its form. Whole-cell biosensors respond selectively to toxic Hg(ii), efficiently transformed by environmental microbes into methylmercury, a highly toxic form that builds up in aquatic animals. Metabolically engineered Escherichia coli (E. coli) have successfully produced rainbow colorants. By de novo reconstruction of the carotenoid synthetic pathway, the Hg(ii)-responsive production of lycopene and β-carotene enabled programmed E. coli to potentially become an optical biosensor for the qualitative and quantitative detection of ecotoxic Hg(ii). The red color of the lycopene-based biosensor cell pellet was visible upon exposure to 49 nM Hg(ii) and above. The orange β-carotene-based biosensor responded to a simple colorimetric assay as low as 12 nM Hg(ii). A linear response was observed at Hg(ii) concentrations ranging from 12 to 195 nM. Importantly, high specificity and good anti-interference capability suggested that metabolic engineering of the carotenoid biosynthesis was an alternative to developing a visual platform for the rapid analysis of the concentration and toxicity of Hg(ii) in environmentally polluted water. The Royal Society of Chemistry 2022-12-16 /pmc/articles/PMC9756418/ /pubmed/36545109 http://dx.doi.org/10.1039/d2ra06764a Text en This journal is © The Royal Society of Chemistry https://creativecommons.org/licenses/by-nc/3.0/
spellingShingle Chemistry
Hui, Chang-ye
Hu, Shun-yu
Li, Li-mei
Yun, Jian-pei
Zhang, Yan-fang
Yi, Juan
Zhang, Nai-xing
Guo, Yan
Metabolic engineering of the carotenoid biosynthetic pathway toward a specific and sensitive inorganic mercury biosensor
title Metabolic engineering of the carotenoid biosynthetic pathway toward a specific and sensitive inorganic mercury biosensor
title_full Metabolic engineering of the carotenoid biosynthetic pathway toward a specific and sensitive inorganic mercury biosensor
title_fullStr Metabolic engineering of the carotenoid biosynthetic pathway toward a specific and sensitive inorganic mercury biosensor
title_full_unstemmed Metabolic engineering of the carotenoid biosynthetic pathway toward a specific and sensitive inorganic mercury biosensor
title_short Metabolic engineering of the carotenoid biosynthetic pathway toward a specific and sensitive inorganic mercury biosensor
title_sort metabolic engineering of the carotenoid biosynthetic pathway toward a specific and sensitive inorganic mercury biosensor
topic Chemistry
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9756418/
https://www.ncbi.nlm.nih.gov/pubmed/36545109
http://dx.doi.org/10.1039/d2ra06764a
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