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Countrywide insecticide resistance monitoring and first report of the presence of the L1014S knock down resistance in Niger, West Africa

BACKGROUND: Mass distribution of insecticide-treated nets (ITNs) is the principal malaria vector control strategy adopted by Niger. To better inform on the most appropriate ITN to distribute, the National Malaria Control Programme (NMCP) of Niger and its partners, conducted insecticide resistance mo...

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Autores principales: Soumaila, Hadiza, Hamani, Boubé, Arzika, Ibrahim Issa, Soumana, Amadou, Daouda, Abdoulaye, Daouda, Fatoumata Abdoulaye, Iro, Souleymane Mahaman, Gouro, Samira, Zaman-Allah, Maman Sani, Mahamadou, Izamné, Kadri, Saadou, Salé, Noura Maman, Hounkanrin, Wilfried, Mahamadou, Boubacar, Zamaka, Halima Naroua, Labbo, Rabiou, Laminou, Ibrahim Maman, Jackou, Hadiza, Idrissa, Sabiti, Coulibaly, Eric, Bahari-Tohon, Zilahatou, Mathieu, Els, Carlson, Jenny, Dotson, Ellen, Awolola, Taiwo Samson, Flatley, Cecilia, Chabi, Joseph
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9756763/
https://www.ncbi.nlm.nih.gov/pubmed/36522727
http://dx.doi.org/10.1186/s12936-022-04410-4
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author Soumaila, Hadiza
Hamani, Boubé
Arzika, Ibrahim Issa
Soumana, Amadou
Daouda, Abdoulaye
Daouda, Fatoumata Abdoulaye
Iro, Souleymane Mahaman
Gouro, Samira
Zaman-Allah, Maman Sani
Mahamadou, Izamné
Kadri, Saadou
Salé, Noura Maman
Hounkanrin, Wilfried
Mahamadou, Boubacar
Zamaka, Halima Naroua
Labbo, Rabiou
Laminou, Ibrahim Maman
Jackou, Hadiza
Idrissa, Sabiti
Coulibaly, Eric
Bahari-Tohon, Zilahatou
Mathieu, Els
Carlson, Jenny
Dotson, Ellen
Awolola, Taiwo Samson
Flatley, Cecilia
Chabi, Joseph
author_facet Soumaila, Hadiza
Hamani, Boubé
Arzika, Ibrahim Issa
Soumana, Amadou
Daouda, Abdoulaye
Daouda, Fatoumata Abdoulaye
Iro, Souleymane Mahaman
Gouro, Samira
Zaman-Allah, Maman Sani
Mahamadou, Izamné
Kadri, Saadou
Salé, Noura Maman
Hounkanrin, Wilfried
Mahamadou, Boubacar
Zamaka, Halima Naroua
Labbo, Rabiou
Laminou, Ibrahim Maman
Jackou, Hadiza
Idrissa, Sabiti
Coulibaly, Eric
Bahari-Tohon, Zilahatou
Mathieu, Els
Carlson, Jenny
Dotson, Ellen
Awolola, Taiwo Samson
Flatley, Cecilia
Chabi, Joseph
author_sort Soumaila, Hadiza
collection PubMed
description BACKGROUND: Mass distribution of insecticide-treated nets (ITNs) is the principal malaria vector control strategy adopted by Niger. To better inform on the most appropriate ITN to distribute, the National Malaria Control Programme (NMCP) of Niger and its partners, conducted insecticide resistance monitoring in selected sites across the country. METHODS: The susceptibility of Anopheles gambiae sensu lato (s.l.) to chlorfenapyr and pyrethroid insecticides was investigated in a total of sixteen sites in 2019 and 2020, using 2–5-day-old adults reared from wild collected larvae per site. The susceptibility status, pyrethroid resistance intensity at 5 and 10 times the diagnostic concentrations, and piperonyl butoxide (PBO) synergism with diagnostic concentrations of deltamethrin, permethrin and alpha-cypermethrin were assessed using WHO bioassays. Two doses (100 and 200 µg/bottle) of chlorfenapyr were tested using the CDC bottle assay method. Species composition and allele frequencies for knock-down resistance (kdr-L1014F and L1014S) and acetylcholinesterase (ace-1 G119S) mutations were further characterized using polymerase chain reaction (PCR). RESULTS: High resistance intensity to all pyrethroids tested was observed in all sites except for alpha-cypermethrin in Gaya and Tessaoua and permethrin in Gaya in 2019 recording moderate resistance intensity. Similarly, Balleyara, Keita and Tillabery yielded moderate resistance intensity for alpha-cypermethrin and deltamethrin, and Niamey V low resistance intensity against deltamethrin and permethrin in 2020. Pre-exposure to PBO substantially increased susceptibility with average increases in mortality between 0 and 70% for tested pyrethroids. Susceptibility to chlorfenapyr (100 µg/bottle) was recorded in all sites except in Tessaoua and Magaria where susceptibility was recorded at the dose of 200 µg/bottle. Anopheles coluzzii was the predominant malaria vector species in most of the sites followed by An. gambiae sensu stricto (s.s.) and Anopheles arabiensis. The kdr-L1014S allele, investigated for the first time, was detected in the country. Both kdr-L1014F (frequencies [0.46–0.81]) and L1014S (frequencies [0.41–0.87]) were present in all sites while the ace-1 G119S was between 0.08 and 0.20. CONCLUSION: The data collected will guide the NMCP in making evidence-based decisions to better adapt vector control strategies and insecticide resistance management in Niger, starting with mass distribution of new generation ITNs such as interceptor G2 and PBO ITNs. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s12936-022-04410-4.
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spelling pubmed-97567632022-12-17 Countrywide insecticide resistance monitoring and first report of the presence of the L1014S knock down resistance in Niger, West Africa Soumaila, Hadiza Hamani, Boubé Arzika, Ibrahim Issa Soumana, Amadou Daouda, Abdoulaye Daouda, Fatoumata Abdoulaye Iro, Souleymane Mahaman Gouro, Samira Zaman-Allah, Maman Sani Mahamadou, Izamné Kadri, Saadou Salé, Noura Maman Hounkanrin, Wilfried Mahamadou, Boubacar Zamaka, Halima Naroua Labbo, Rabiou Laminou, Ibrahim Maman Jackou, Hadiza Idrissa, Sabiti Coulibaly, Eric Bahari-Tohon, Zilahatou Mathieu, Els Carlson, Jenny Dotson, Ellen Awolola, Taiwo Samson Flatley, Cecilia Chabi, Joseph Malar J Research BACKGROUND: Mass distribution of insecticide-treated nets (ITNs) is the principal malaria vector control strategy adopted by Niger. To better inform on the most appropriate ITN to distribute, the National Malaria Control Programme (NMCP) of Niger and its partners, conducted insecticide resistance monitoring in selected sites across the country. METHODS: The susceptibility of Anopheles gambiae sensu lato (s.l.) to chlorfenapyr and pyrethroid insecticides was investigated in a total of sixteen sites in 2019 and 2020, using 2–5-day-old adults reared from wild collected larvae per site. The susceptibility status, pyrethroid resistance intensity at 5 and 10 times the diagnostic concentrations, and piperonyl butoxide (PBO) synergism with diagnostic concentrations of deltamethrin, permethrin and alpha-cypermethrin were assessed using WHO bioassays. Two doses (100 and 200 µg/bottle) of chlorfenapyr were tested using the CDC bottle assay method. Species composition and allele frequencies for knock-down resistance (kdr-L1014F and L1014S) and acetylcholinesterase (ace-1 G119S) mutations were further characterized using polymerase chain reaction (PCR). RESULTS: High resistance intensity to all pyrethroids tested was observed in all sites except for alpha-cypermethrin in Gaya and Tessaoua and permethrin in Gaya in 2019 recording moderate resistance intensity. Similarly, Balleyara, Keita and Tillabery yielded moderate resistance intensity for alpha-cypermethrin and deltamethrin, and Niamey V low resistance intensity against deltamethrin and permethrin in 2020. Pre-exposure to PBO substantially increased susceptibility with average increases in mortality between 0 and 70% for tested pyrethroids. Susceptibility to chlorfenapyr (100 µg/bottle) was recorded in all sites except in Tessaoua and Magaria where susceptibility was recorded at the dose of 200 µg/bottle. Anopheles coluzzii was the predominant malaria vector species in most of the sites followed by An. gambiae sensu stricto (s.s.) and Anopheles arabiensis. The kdr-L1014S allele, investigated for the first time, was detected in the country. Both kdr-L1014F (frequencies [0.46–0.81]) and L1014S (frequencies [0.41–0.87]) were present in all sites while the ace-1 G119S was between 0.08 and 0.20. CONCLUSION: The data collected will guide the NMCP in making evidence-based decisions to better adapt vector control strategies and insecticide resistance management in Niger, starting with mass distribution of new generation ITNs such as interceptor G2 and PBO ITNs. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s12936-022-04410-4. BioMed Central 2022-12-16 /pmc/articles/PMC9756763/ /pubmed/36522727 http://dx.doi.org/10.1186/s12936-022-04410-4 Text en © The Author(s) 2022 https://creativecommons.org/licenses/by/4.0/Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/ (https://creativecommons.org/publicdomain/zero/1.0/) ) applies to the data made available in this article, unless otherwise stated in a credit line to the data.
spellingShingle Research
Soumaila, Hadiza
Hamani, Boubé
Arzika, Ibrahim Issa
Soumana, Amadou
Daouda, Abdoulaye
Daouda, Fatoumata Abdoulaye
Iro, Souleymane Mahaman
Gouro, Samira
Zaman-Allah, Maman Sani
Mahamadou, Izamné
Kadri, Saadou
Salé, Noura Maman
Hounkanrin, Wilfried
Mahamadou, Boubacar
Zamaka, Halima Naroua
Labbo, Rabiou
Laminou, Ibrahim Maman
Jackou, Hadiza
Idrissa, Sabiti
Coulibaly, Eric
Bahari-Tohon, Zilahatou
Mathieu, Els
Carlson, Jenny
Dotson, Ellen
Awolola, Taiwo Samson
Flatley, Cecilia
Chabi, Joseph
Countrywide insecticide resistance monitoring and first report of the presence of the L1014S knock down resistance in Niger, West Africa
title Countrywide insecticide resistance monitoring and first report of the presence of the L1014S knock down resistance in Niger, West Africa
title_full Countrywide insecticide resistance monitoring and first report of the presence of the L1014S knock down resistance in Niger, West Africa
title_fullStr Countrywide insecticide resistance monitoring and first report of the presence of the L1014S knock down resistance in Niger, West Africa
title_full_unstemmed Countrywide insecticide resistance monitoring and first report of the presence of the L1014S knock down resistance in Niger, West Africa
title_short Countrywide insecticide resistance monitoring and first report of the presence of the L1014S knock down resistance in Niger, West Africa
title_sort countrywide insecticide resistance monitoring and first report of the presence of the l1014s knock down resistance in niger, west africa
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9756763/
https://www.ncbi.nlm.nih.gov/pubmed/36522727
http://dx.doi.org/10.1186/s12936-022-04410-4
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