Cargando…

Construction and analysis of expression profile of exosomal lncRNAs in pleural effusion in lung adenocarcinoma

BACKGROUND: Lung adenocarcinoma (LUAD) is a highly malignant tumor with a very low five‐year survival rate. In this study, we aimed to identify differentially expressed long‐chain non‐coding RNA (lncRNAs) and mRNAs from benign and malignant pleural effusion exosomes. METHODS: We used gene microassay...

Descripción completa

Detalles Bibliográficos
Autores principales: Huang, Xiaolu, Zhou, Huixin, Yang, Xiang, Shi, Wenjing, Hu, Lijuan, Wang, Junjun, Zhang, Fan, Shao, Fanggui, Zhang, Meijuan, Jiang, Feng, Wang, Yumin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9756994/
https://www.ncbi.nlm.nih.gov/pubmed/36426920
http://dx.doi.org/10.1002/jcla.24777
_version_ 1784851736192614400
author Huang, Xiaolu
Zhou, Huixin
Yang, Xiang
Shi, Wenjing
Hu, Lijuan
Wang, Junjun
Zhang, Fan
Shao, Fanggui
Zhang, Meijuan
Jiang, Feng
Wang, Yumin
author_facet Huang, Xiaolu
Zhou, Huixin
Yang, Xiang
Shi, Wenjing
Hu, Lijuan
Wang, Junjun
Zhang, Fan
Shao, Fanggui
Zhang, Meijuan
Jiang, Feng
Wang, Yumin
author_sort Huang, Xiaolu
collection PubMed
description BACKGROUND: Lung adenocarcinoma (LUAD) is a highly malignant tumor with a very low five‐year survival rate. In this study, we aimed to identify differentially expressed long‐chain non‐coding RNA (lncRNAs) and mRNAs from benign and malignant pleural effusion exosomes. METHODS: We used gene microassay and quantitative real‐time reverse transcription polymerase chain reaction (RT‐qPCR) to detect and verify differentially expressed mRNAs and lncRNAs in benign and malignant pleural effusion exosomes. Gene Ontology (GO) functional significance and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway significance enrichment analyses were performed to identify the difference in biological processes and functions between different mRNAs. We selected the lncRNA ZBED5‐AS1 with an upregulated differential fold of 3.003 and conducted a preliminary study on its cellular function. RESULTS: Gene microassay results revealed that 177 differentially expressed lncRNAs were upregulated, and 215 were downregulated. The top 10 upregulated were FMN1, AL118505.1, LINC00452, AL109811.2, CATG00000040683.1, AC137932.1, AC008619.1, AL450344.1, AC092718.6, and ZBED5‐AS1. The top 10 downregulated were TEX41, G067726, JAZF1‐AS1, AC027328.1, AL445645.1, AL022345.4, AC008572.1, AC123777.1, AC093714.1, and PHKG1. For the mRNAs, 79 were upregulated, and 123 were notably downregulated. GO analysis revealed that the upregulated differential mRNAs were mainly involved in “cellular response to acidic pH” (biological processes), “endoplasmic reticulum part” (cellular components), and “at DNA binding, cyclase activity” (molecular functions). KEGG pathways were found to be related to V. cholerae infection, Parkinson's disease, and cell adhesion molecules. RT‐qPCR showed that ZBED5‐AS1 was highly expressed in LUAD tissues, cells, and benign and malignant pleural fluid exosomes. Overexpression of ZBED5‐AS1 could significantly promote the proliferation, migration, invasion, and colony formation of LUAD cells, and knockdown had the opposite consequence. CONCLUSION: The pleural effusion exosomes from patients with LUAD include several improperly expressed genes, and lncRNA‐ZBED5‐AS1 is a new biomarker that aids in our understanding of the occurrence and progression of LUAD.
format Online
Article
Text
id pubmed-9756994
institution National Center for Biotechnology Information
language English
publishDate 2022
publisher John Wiley and Sons Inc.
record_format MEDLINE/PubMed
spelling pubmed-97569942022-12-20 Construction and analysis of expression profile of exosomal lncRNAs in pleural effusion in lung adenocarcinoma Huang, Xiaolu Zhou, Huixin Yang, Xiang Shi, Wenjing Hu, Lijuan Wang, Junjun Zhang, Fan Shao, Fanggui Zhang, Meijuan Jiang, Feng Wang, Yumin J Clin Lab Anal Research Articles BACKGROUND: Lung adenocarcinoma (LUAD) is a highly malignant tumor with a very low five‐year survival rate. In this study, we aimed to identify differentially expressed long‐chain non‐coding RNA (lncRNAs) and mRNAs from benign and malignant pleural effusion exosomes. METHODS: We used gene microassay and quantitative real‐time reverse transcription polymerase chain reaction (RT‐qPCR) to detect and verify differentially expressed mRNAs and lncRNAs in benign and malignant pleural effusion exosomes. Gene Ontology (GO) functional significance and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway significance enrichment analyses were performed to identify the difference in biological processes and functions between different mRNAs. We selected the lncRNA ZBED5‐AS1 with an upregulated differential fold of 3.003 and conducted a preliminary study on its cellular function. RESULTS: Gene microassay results revealed that 177 differentially expressed lncRNAs were upregulated, and 215 were downregulated. The top 10 upregulated were FMN1, AL118505.1, LINC00452, AL109811.2, CATG00000040683.1, AC137932.1, AC008619.1, AL450344.1, AC092718.6, and ZBED5‐AS1. The top 10 downregulated were TEX41, G067726, JAZF1‐AS1, AC027328.1, AL445645.1, AL022345.4, AC008572.1, AC123777.1, AC093714.1, and PHKG1. For the mRNAs, 79 were upregulated, and 123 were notably downregulated. GO analysis revealed that the upregulated differential mRNAs were mainly involved in “cellular response to acidic pH” (biological processes), “endoplasmic reticulum part” (cellular components), and “at DNA binding, cyclase activity” (molecular functions). KEGG pathways were found to be related to V. cholerae infection, Parkinson's disease, and cell adhesion molecules. RT‐qPCR showed that ZBED5‐AS1 was highly expressed in LUAD tissues, cells, and benign and malignant pleural fluid exosomes. Overexpression of ZBED5‐AS1 could significantly promote the proliferation, migration, invasion, and colony formation of LUAD cells, and knockdown had the opposite consequence. CONCLUSION: The pleural effusion exosomes from patients with LUAD include several improperly expressed genes, and lncRNA‐ZBED5‐AS1 is a new biomarker that aids in our understanding of the occurrence and progression of LUAD. John Wiley and Sons Inc. 2022-11-25 /pmc/articles/PMC9756994/ /pubmed/36426920 http://dx.doi.org/10.1002/jcla.24777 Text en © 2022 The Authors. Journal of Clinical Laboratory Analysis published by Wiley Periodicals LLC. https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the terms of the http://creativecommons.org/licenses/by-nc-nd/4.0/ (https://creativecommons.org/licenses/by-nc-nd/4.0/) License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non‐commercial and no modifications or adaptations are made.
spellingShingle Research Articles
Huang, Xiaolu
Zhou, Huixin
Yang, Xiang
Shi, Wenjing
Hu, Lijuan
Wang, Junjun
Zhang, Fan
Shao, Fanggui
Zhang, Meijuan
Jiang, Feng
Wang, Yumin
Construction and analysis of expression profile of exosomal lncRNAs in pleural effusion in lung adenocarcinoma
title Construction and analysis of expression profile of exosomal lncRNAs in pleural effusion in lung adenocarcinoma
title_full Construction and analysis of expression profile of exosomal lncRNAs in pleural effusion in lung adenocarcinoma
title_fullStr Construction and analysis of expression profile of exosomal lncRNAs in pleural effusion in lung adenocarcinoma
title_full_unstemmed Construction and analysis of expression profile of exosomal lncRNAs in pleural effusion in lung adenocarcinoma
title_short Construction and analysis of expression profile of exosomal lncRNAs in pleural effusion in lung adenocarcinoma
title_sort construction and analysis of expression profile of exosomal lncrnas in pleural effusion in lung adenocarcinoma
topic Research Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9756994/
https://www.ncbi.nlm.nih.gov/pubmed/36426920
http://dx.doi.org/10.1002/jcla.24777
work_keys_str_mv AT huangxiaolu constructionandanalysisofexpressionprofileofexosomallncrnasinpleuraleffusioninlungadenocarcinoma
AT zhouhuixin constructionandanalysisofexpressionprofileofexosomallncrnasinpleuraleffusioninlungadenocarcinoma
AT yangxiang constructionandanalysisofexpressionprofileofexosomallncrnasinpleuraleffusioninlungadenocarcinoma
AT shiwenjing constructionandanalysisofexpressionprofileofexosomallncrnasinpleuraleffusioninlungadenocarcinoma
AT hulijuan constructionandanalysisofexpressionprofileofexosomallncrnasinpleuraleffusioninlungadenocarcinoma
AT wangjunjun constructionandanalysisofexpressionprofileofexosomallncrnasinpleuraleffusioninlungadenocarcinoma
AT zhangfan constructionandanalysisofexpressionprofileofexosomallncrnasinpleuraleffusioninlungadenocarcinoma
AT shaofanggui constructionandanalysisofexpressionprofileofexosomallncrnasinpleuraleffusioninlungadenocarcinoma
AT zhangmeijuan constructionandanalysisofexpressionprofileofexosomallncrnasinpleuraleffusioninlungadenocarcinoma
AT jiangfeng constructionandanalysisofexpressionprofileofexosomallncrnasinpleuraleffusioninlungadenocarcinoma
AT wangyumin constructionandanalysisofexpressionprofileofexosomallncrnasinpleuraleffusioninlungadenocarcinoma