Cargando…

Two Leishmania species separation targeting the ITS-rDNA and Cyt b genes by developing and evaluating HRM- qPCR

BACKGROUND: Incidence of Cutaneous Leishmaniasis as an infectious and neglected disease is increasing, for the diagnosis of which several traditional methods and conventional PCR techniques have been developed, employing different genes for species identification. METHODS: Leishmania parasites were...

Descripción completa

Detalles Bibliográficos
Autores principales:	Alaeenovin, Elnaz, Parvizi, Parviz, Ghafari, Seyedeh Maryam
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Sociedade Brasileira de Medicina Tropical - SBMT 2022
Materias:	Major Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9757716/ https://www.ncbi.nlm.nih.gov/pubmed/36542013 http://dx.doi.org/10.1590/0037-8682-0186-2022

Ejemplares similares

Sensitivity comparison for the Leishmania spp. detection in different canine tissues using PCR-HRM
por: Garay, Ana Fidelina Gómez, et al.
Publicado: (2022)

Accurate Identification of Leishmania Parasites in Sand Flies by Polymorphism Analysis of Cytochrome Oxidase Subunit 2 Gene Using Polymerase Chain Reaction and Quantitative PCR-High Resolution Melting Techniques in Iranian Border with Iraq
por: Ghafari, Seyedeh Maryam, et al.
Publicado: (2022)

Developing, Modifying, and Validating a TaqMan Real-Time PCR Technique for Accurate Identification of Leishmania Parasites Causing Most Leishmaniasis in Iran
por: Fotouhi-Ardakani, Reza, et al.
Publicado: (2021)

Genome Size, rDNA Copy, and qPCR Assays for Symbiodiniaceae
por: Saad, Osama S., et al.
Publicado: (2020)

Detection and quantification of Spirocerca lupi by HRM qPCR in fecal samples from dogs with spirocercosis
por: Rojas, Alicia, et al.
Publicado: (2017)

A novel 5-Plex qPCR-HRM assay detecting human diarrheal parasites
por: Lamien-Meda, Aline, et al.
Publicado: (2020)

Evaluative Assay of Nuclear and Mitochondrial Genes to Diagnose Leishmania Species in Clinical Specimens
por: ESMAEILI RASTAGHI, Ahmad Reza, et al.
Publicado: (2017)

The use of HRM shifts in qPCR to investigate a much neglected aspect of interference by intracellular nanoparticles
por: Sanabria, Natasha M., et al.
Publicado: (2021)

RT-qPCR half-reaction optimization for the detection of SARS-CoV-2
por: Wink, Priscila Lamb, et al.
Publicado: (2021)

Comparison and clinical validation of qPCR assays targeting Leishmania 18S rDNA and HSP70 genes in patients with American Tegumentary Leishmaniasis
por: Filgueira, Camila Patricio Braga, et al.
Publicado: (2020)

Detection of SARS-CoV-2 spike protein D614G mutation by qPCR-HRM analysis
por: Gazali, Faris Muhammad, et al.
Publicado: (2021)

Evaluation of RT-qPCR of mouthwash and buccal swabs for detection of SARS-CoV-2 in children and adults
por: Laferl, Hermann, et al.
Publicado: (2022)

Predominance of Leishmania major and rare occurrence of Leishmania tropica with haplotype variability at the center of Iran
por: Zahirnia, Amir Hossein, et al.
Publicado: (2018)

A novel multiplex qPCR‑HRM assay for the simultaneous detection of four abortive zoonotic agents in cattle, sheep, and goats
por: Modise, Boitumelo M., et al.
Publicado: (2023)

Data on optimisation of a multiplex HRM-qPCR assay for native and invasive crayfish as well as the crayfish plague in four river catchments
por: Robinson, Chloe Victoria, et al.
Publicado: (2018)

Simultaneous detection of downy mildew and powdery mildew pathogens on Cucumis sativus and other cucurbits using duplex-qPCR and HRM analysis
por: Bandamaravuri, Kishore Babu, et al.
Publicado: (2020)

Species-level identification of trypanosomes infecting Australian wildlife by High-Resolution Melting - Real Time Quantitative Polymerase Chain Reaction (HRM-qPCR)
por: Keatley, S., et al.
Publicado: (2020)

The ‘PREXCEL-Q Method’ for qPCR
por: Gallup, Jack M., et al.
Publicado: (2008)

Detection of Leishmania spp. in Cats: Analysis of Nasal, Oral and Conjunctival Swabs by PCR and HRM
por: Alves-Martin, Maria Fernanda, et al.
Publicado: (2023)

Comparative Studies of 5S rDNA Profiles and Cyt b Sequences in two Onychostoma Species (Cyprinidae)
por: Han, Chiao-Chuan, et al.
Publicado: (2015)

A novel multiplex qPCR targeting 23S rDNA for diagnosis of swine dysentery and porcine intestinal spirochaetosis
por: Borgström, Anna, et al.
Publicado: (2017)

Evaluation of a kDNA-Based qPCR Assay for the Detection and Quantification of Old World Leishmania Species
por: Ceccarelli, Marcello, et al.
Publicado: (2020)

Evaluation of sxtA and rDNA qPCR assays through monitoring of an inshore bloom of Alexandrium catenella Group 1
por: Murray, Shauna A., et al.
Publicado: (2019)

High variability in SSU rDNA gene copy number among planktonic foraminifera revealed by single-cell qPCR
por: Milivojević, Tamara, et al.
Publicado: (2021)

Universal probe-based intermediate primer-triggered qPCR (UPIP-qPCR) for SNP genotyping
por: Li, Baowei, et al.
Publicado: (2021)

Analytical Performance of Four Polymerase Chain Reaction (PCR) and Real Time PCR (qPCR) Assays for the Detection of Six Leishmania Species DNA in Colombia
por: León, Cielo M., et al.
Publicado: (2017)

HRM Accuracy and Limitations as a Species Typing Tool for Leishmania Parasites
por: Filgueira, Camila Patricio Braga, et al.
Publicado: (2023)

Differentiation of Leishmania (L.) infantum, Leishmania (L.) amazonensis and Leishmania (L.) mexicana Using Sequential qPCR Assays and High-Resolution Melt Analysis
por: Ceccarelli, Marcello, et al.
Publicado: (2020)

Semi-Quantitative, Duplexed qPCR Assay for the Detection of Leishmania spp. Using Bisulphite Conversion Technology
por: Gow, Ineka, et al.
Publicado: (2019)

Validation of kinetics similarity in qPCR
por: Bar, Tzachi, et al.
Publicado: (2012)

On non-detects in qPCR data
por: McCall, Matthew N., et al.
Publicado: (2014)

qPCR primer design revisited
por: Bustin, Stephen, et al.
Publicado: (2017)

Contribution of the qPCR for the Diagnosis of Pneumocystosis
por: Issa, Nahema, et al.
Publicado: (2017)

The kinetic requirements of extreme qPCR
por: Millington, Adam L., et al.
Publicado: (2019)

A common base method for analysis of qPCR data and the application of simple blocking in qPCR experiments
por: Ganger, Michael T., et al.
Publicado: (2017)

Auto-qPCR; a python-based web app for automated and reproducible analysis of qPCR data
por: Maussion, Gilles, et al.
Publicado: (2021)

Artificial Substrates Coupled with qPCR (AS-qPCR) Assay for the Detection of the Toxic Benthopelagic Dinoflagellate Vulcanodinium rugosum
por: Bouquet, Aurélien, et al.
Publicado: (2023)

qPCR, dPCR, NGS – A journey
por: Huggett, Jim F., et al.
Publicado: (2015)

Performance of a universal PCR assay to identify different Leishmania species causative of Old World cutaneous leishmaniasis
por: Nateghi Rostami, Mahmoud, et al.
Publicado: (2020)

TqPCR: A Touchdown qPCR Assay with Significantly Improved Detection Sensitivity and Amplification Efficiency of SYBR Green qPCR
por: Zhang, Qian, et al.
Publicado: (2015)

Cannot write session to /tmp/vufind_sessions/sess_ne5lpohf2v56lf90tnrdea90a7