Impact of Hyaluronic Acid on the Viability of Mesenchymal Cells Derived from Adipose Tissue Grown in Collagen Type I/III Membrane

Objective  To evaluate in vitro the viability of mesenchymal stem cells derived from adipose tissue (AD-MSCs) in different commercial solutions of hyaluronic acid (HA) before and after being sowed in collagen I/III membrane. Methods  In the first stage, the interaction between AD-MSCs was analyzed w...

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Autores principales: Kaleka, Camila Cohen, Debieux, Pedro, Antonioli, Eliane, Zucconi, Eder, Cohen, Moisés, Ferretti, Mário
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Thieme Revinter Publicações Ltda. 2022
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9757957/
https://www.ncbi.nlm.nih.gov/pubmed/36540744
http://dx.doi.org/10.1055/s-0041-1740198
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author Kaleka, Camila Cohen
Debieux, Pedro
Antonioli, Eliane
Zucconi, Eder
Cohen, Moisés
Ferretti, Mário
author_facet Kaleka, Camila Cohen
Debieux, Pedro
Antonioli, Eliane
Zucconi, Eder
Cohen, Moisés
Ferretti, Mário
author_sort Kaleka, Camila Cohen
collection PubMed
description Objective  To evaluate in vitro the viability of mesenchymal stem cells derived from adipose tissue (AD-MSCs) in different commercial solutions of hyaluronic acid (HA) before and after being sowed in collagen I/III membrane. Methods  In the first stage, the interaction between AD-MSCs was analyzed with seven different commercial products of HA, phosphate buffered saline (PBS), and bovine fetal serum (BFS), performed by counting living and dead cells after 24, 48 and 72 hours. Five products with a higher number of living cells were selected and the interaction between HA with AD-MSCs and type I/III collagen membrane was evaluated by counting living and dead cells in the same time interval (24, 48 and 72 hours). Results  In both situations analyzed (HA + AD-MSCs and HA + AD-MSCs + membrane), BFS presented the highest percentage of living cells after 24, 48 and 72 hours, a result higher than that of HA. Conclusion  The association of HA with AD-MSCs, with or without membrane, showed no superiority in cell viability when compared with BFS.
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spelling pubmed-97579572022-12-19 Impact of Hyaluronic Acid on the Viability of Mesenchymal Cells Derived from Adipose Tissue Grown in Collagen Type I/III Membrane Kaleka, Camila Cohen Debieux, Pedro Antonioli, Eliane Zucconi, Eder Cohen, Moisés Ferretti, Mário Rev Bras Ortop (Sao Paulo) Objective  To evaluate in vitro the viability of mesenchymal stem cells derived from adipose tissue (AD-MSCs) in different commercial solutions of hyaluronic acid (HA) before and after being sowed in collagen I/III membrane. Methods  In the first stage, the interaction between AD-MSCs was analyzed with seven different commercial products of HA, phosphate buffered saline (PBS), and bovine fetal serum (BFS), performed by counting living and dead cells after 24, 48 and 72 hours. Five products with a higher number of living cells were selected and the interaction between HA with AD-MSCs and type I/III collagen membrane was evaluated by counting living and dead cells in the same time interval (24, 48 and 72 hours). Results  In both situations analyzed (HA + AD-MSCs and HA + AD-MSCs + membrane), BFS presented the highest percentage of living cells after 24, 48 and 72 hours, a result higher than that of HA. Conclusion  The association of HA with AD-MSCs, with or without membrane, showed no superiority in cell viability when compared with BFS. Thieme Revinter Publicações Ltda. 2022-02-09 /pmc/articles/PMC9757957/ /pubmed/36540744 http://dx.doi.org/10.1055/s-0041-1740198 Text en Sociedade Brasileira de Ortopedia e Traumatologia. This is an open access article published by Thieme under the terms of the Creative Commons Attribution-NonDerivative-NonCommercial License, permitting copying and reproduction so long as the original work is given appropriate credit. Contents may not be used for commecial purposes, or adapted, remixed, transformed or built upon. ( https://creativecommons.org/licenses/by-nc-nd/4.0/ ) https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivatives License, which permits unrestricted reproduction and distribution, for non-commercial purposes only; and use and reproduction, but not distribution, of adapted material for non-commercial purposes only, provided the original work is properly cited.
spellingShingle Kaleka, Camila Cohen
Debieux, Pedro
Antonioli, Eliane
Zucconi, Eder
Cohen, Moisés
Ferretti, Mário
Impact of Hyaluronic Acid on the Viability of Mesenchymal Cells Derived from Adipose Tissue Grown in Collagen Type I/III Membrane
title Impact of Hyaluronic Acid on the Viability of Mesenchymal Cells Derived from Adipose Tissue Grown in Collagen Type I/III Membrane
title_full Impact of Hyaluronic Acid on the Viability of Mesenchymal Cells Derived from Adipose Tissue Grown in Collagen Type I/III Membrane
title_fullStr Impact of Hyaluronic Acid on the Viability of Mesenchymal Cells Derived from Adipose Tissue Grown in Collagen Type I/III Membrane
title_full_unstemmed Impact of Hyaluronic Acid on the Viability of Mesenchymal Cells Derived from Adipose Tissue Grown in Collagen Type I/III Membrane
title_short Impact of Hyaluronic Acid on the Viability of Mesenchymal Cells Derived from Adipose Tissue Grown in Collagen Type I/III Membrane
title_sort impact of hyaluronic acid on the viability of mesenchymal cells derived from adipose tissue grown in collagen type i/iii membrane
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9757957/
https://www.ncbi.nlm.nih.gov/pubmed/36540744
http://dx.doi.org/10.1055/s-0041-1740198
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