Simultaneous loading of PCR-based multiple fragments on mouse artificial chromosome vectors in DT40 cell for gene delivery

Homology-directed repair-mediated knock-in (HDR-KI) in combination with CRISPR-Cas9-mediated double strand break (DSB) leads to high frequency of site-specific HDR-KI. While this characteristic is advantageous for generating genetically modified cellular and animal models, HDR-KI efficiency in mamma...

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Detalles Bibliográficos
Autores principales: Yamazaki, Kyotaro, Matsuo, Kyosuke, Okada, Akane, Uno, Narumi, Suzuki, Teruhiko, Abe, Satoshi, Hamamichi, Shusei, Kishima, Nanami, Togai, Shota, Tomizuka, Kazuma, Kazuki, Yasuhiro
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2022
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9758134/
https://www.ncbi.nlm.nih.gov/pubmed/36526651
http://dx.doi.org/10.1038/s41598-022-25959-9

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