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Reclassification of eight Akkermansia muciniphila strains and description of Akkermansia massiliensis sp. nov. and Candidatus Akkermansia timonensis, isolated from human feces
Akkermansia muciniphila is a human intestinal tract bacterium that plays an important role in the mucus layer renewal. Several studies have demonstrated that it is a modulator for gut homeostasis and a probiotic for human health. The Akkermansia genus contains two species with standing in nomenclatu...
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Nature Publishing Group UK
2022
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9758162/ https://www.ncbi.nlm.nih.gov/pubmed/36526682 http://dx.doi.org/10.1038/s41598-022-25873-0 |
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author | Ndongo, Sokhna Armstrong, Nicholas Raoult, Didier Fournier, Pierre-Edouard |
author_facet | Ndongo, Sokhna Armstrong, Nicholas Raoult, Didier Fournier, Pierre-Edouard |
author_sort | Ndongo, Sokhna |
collection | PubMed |
description | Akkermansia muciniphila is a human intestinal tract bacterium that plays an important role in the mucus layer renewal. Several studies have demonstrated that it is a modulator for gut homeostasis and a probiotic for human health. The Akkermansia genus contains two species with standing in nomenclature but their genomic diversity remains unclear. In this study, eight new Akkermansia sp. strains were isolated from the human gut. Using the digital DNA-DNA hybridization (dDDH), average nucleotide identity (ANI) and core genome-based phylogenetic analysis applied to 104 A. muciniphila whole genomes sequences, strains were reclassified into three clusters. Cluster I groups A. muciniphila strains (including strain ATCC BAA-835(T) as type strain), whereas clusters II and III represent two new species. A member of cluster II, strain Marseille-P6666 differed from A. muciniphila strain ATCC BAA-835(T) and from A. glycaniphila strain Pyt(T) in its ability to grow in microaerophilic atmosphere up to 42 °C, to assimilate various carbon sources and to produce acids from a several compounds. The major fatty acids of strain Marseille-P6666 were 12-methyl-tetradecanoic and pentadecanoic acids. The DNA G + C content of strain Marseille-P6666 was 57.8%. On the basis of these properties, we propose the name A. massiliensis sp. nov. for members of cluster II, with strain Marseille-P6666(T) (= CSUR P6666 = CECT 30548) as type strain. We also propose the name “Candidatus Akkermansia timonensis” sp. nov. for the members of cluster III, which contains only uncultivated strains, strain Akk0196 being the type strain. |
format | Online Article Text |
id | pubmed-9758162 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-97581622022-12-18 Reclassification of eight Akkermansia muciniphila strains and description of Akkermansia massiliensis sp. nov. and Candidatus Akkermansia timonensis, isolated from human feces Ndongo, Sokhna Armstrong, Nicholas Raoult, Didier Fournier, Pierre-Edouard Sci Rep Article Akkermansia muciniphila is a human intestinal tract bacterium that plays an important role in the mucus layer renewal. Several studies have demonstrated that it is a modulator for gut homeostasis and a probiotic for human health. The Akkermansia genus contains two species with standing in nomenclature but their genomic diversity remains unclear. In this study, eight new Akkermansia sp. strains were isolated from the human gut. Using the digital DNA-DNA hybridization (dDDH), average nucleotide identity (ANI) and core genome-based phylogenetic analysis applied to 104 A. muciniphila whole genomes sequences, strains were reclassified into three clusters. Cluster I groups A. muciniphila strains (including strain ATCC BAA-835(T) as type strain), whereas clusters II and III represent two new species. A member of cluster II, strain Marseille-P6666 differed from A. muciniphila strain ATCC BAA-835(T) and from A. glycaniphila strain Pyt(T) in its ability to grow in microaerophilic atmosphere up to 42 °C, to assimilate various carbon sources and to produce acids from a several compounds. The major fatty acids of strain Marseille-P6666 were 12-methyl-tetradecanoic and pentadecanoic acids. The DNA G + C content of strain Marseille-P6666 was 57.8%. On the basis of these properties, we propose the name A. massiliensis sp. nov. for members of cluster II, with strain Marseille-P6666(T) (= CSUR P6666 = CECT 30548) as type strain. We also propose the name “Candidatus Akkermansia timonensis” sp. nov. for the members of cluster III, which contains only uncultivated strains, strain Akk0196 being the type strain. Nature Publishing Group UK 2022-12-16 /pmc/articles/PMC9758162/ /pubmed/36526682 http://dx.doi.org/10.1038/s41598-022-25873-0 Text en © The Author(s) 2022 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . |
spellingShingle | Article Ndongo, Sokhna Armstrong, Nicholas Raoult, Didier Fournier, Pierre-Edouard Reclassification of eight Akkermansia muciniphila strains and description of Akkermansia massiliensis sp. nov. and Candidatus Akkermansia timonensis, isolated from human feces |
title | Reclassification of eight Akkermansia
muciniphila strains and description of Akkermansia
massiliensis sp. nov. and Candidatus Akkermansia timonensis, isolated from human feces |
title_full | Reclassification of eight Akkermansia
muciniphila strains and description of Akkermansia
massiliensis sp. nov. and Candidatus Akkermansia timonensis, isolated from human feces |
title_fullStr | Reclassification of eight Akkermansia
muciniphila strains and description of Akkermansia
massiliensis sp. nov. and Candidatus Akkermansia timonensis, isolated from human feces |
title_full_unstemmed | Reclassification of eight Akkermansia
muciniphila strains and description of Akkermansia
massiliensis sp. nov. and Candidatus Akkermansia timonensis, isolated from human feces |
title_short | Reclassification of eight Akkermansia
muciniphila strains and description of Akkermansia
massiliensis sp. nov. and Candidatus Akkermansia timonensis, isolated from human feces |
title_sort | reclassification of eight akkermansia
muciniphila strains and description of akkermansia
massiliensis sp. nov. and candidatus akkermansia timonensis, isolated from human feces |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9758162/ https://www.ncbi.nlm.nih.gov/pubmed/36526682 http://dx.doi.org/10.1038/s41598-022-25873-0 |
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