Detecting and quantifying liquid–liquid phase separation in living cells by model-free calibrated half-bleaching

Cells contain numerous substructures that have been proposed to form via liquid–liquid phase separation (LLPS). It is currently debated how to reliably distinguish LLPS from other mechanisms. Here, we benchmark different methods using well-controlled model systems in vitro and in living cells. We fi...

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Detalles Bibliográficos
Autores principales: Muzzopappa, Fernando, Hummert, Johan, Anfossi, Michela, Tashev, Stanimir Asenov, Herten, Dirk-Peter, Erdel, Fabian
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2022
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9758202/
https://www.ncbi.nlm.nih.gov/pubmed/36526633
http://dx.doi.org/10.1038/s41467-022-35430-y

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