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Ultrastructural and molecular approach as a tool for taxonomic identification of aquatic macroinvertebrates: A review

Aquatic insects require water at one or other phase for the completion of their life cycle. The insect larvae serve as food for larger invertebrates and vertebrates in aquatic food chain. Their diversity, number, and abundance act as water quality indicators, and thus species are classified accordin...

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Autores principales: Grover, Aseem, Sharma, Parul, Sharma, Radhika, Sinha, Reshma
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9758433/
https://www.ncbi.nlm.nih.gov/pubmed/36536920
http://dx.doi.org/10.1016/j.heliyon.2022.e12236
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author Grover, Aseem
Sharma, Parul
Sharma, Radhika
Sinha, Reshma
author_facet Grover, Aseem
Sharma, Parul
Sharma, Radhika
Sinha, Reshma
author_sort Grover, Aseem
collection PubMed
description Aquatic insects require water at one or other phase for the completion of their life cycle. The insect larvae serve as food for larger invertebrates and vertebrates in aquatic food chain. Their diversity, number, and abundance act as water quality indicators, and thus species are classified accordingly as pollution tolerant or sensitive. So, identifying these aquatic larvae and macroinvertebrates are important for determining the biodiversity, and classification of insect species, followed by assessment of water health, and understanding the influence of climate change and anthropogenetic activities on these. Chances of misidentification have been reported due to loss of expertise, absence of taxonomic keys for larvae or intermediate stages, or damaged structure during collection or preservation. Recent advances in molecular and electron microscopy have revolutionized the identification procedure. Scanning electron microscopy detail the structure and morphology of the insect, while molecular techniques employing PCRs, DNA barcoding, and molecular markers allow the identification of the insects from any tissue (whole/part), and comparing the nucleotide sequences helps in the evaluating the family tree and lineage. The review summarizes the present status of aquatic invertebrates identification and the importance of these two techniques in the taxonomic identification of aquatic insects.
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spelling pubmed-97584332022-12-18 Ultrastructural and molecular approach as a tool for taxonomic identification of aquatic macroinvertebrates: A review Grover, Aseem Sharma, Parul Sharma, Radhika Sinha, Reshma Heliyon Review Article Aquatic insects require water at one or other phase for the completion of their life cycle. The insect larvae serve as food for larger invertebrates and vertebrates in aquatic food chain. Their diversity, number, and abundance act as water quality indicators, and thus species are classified accordingly as pollution tolerant or sensitive. So, identifying these aquatic larvae and macroinvertebrates are important for determining the biodiversity, and classification of insect species, followed by assessment of water health, and understanding the influence of climate change and anthropogenetic activities on these. Chances of misidentification have been reported due to loss of expertise, absence of taxonomic keys for larvae or intermediate stages, or damaged structure during collection or preservation. Recent advances in molecular and electron microscopy have revolutionized the identification procedure. Scanning electron microscopy detail the structure and morphology of the insect, while molecular techniques employing PCRs, DNA barcoding, and molecular markers allow the identification of the insects from any tissue (whole/part), and comparing the nucleotide sequences helps in the evaluating the family tree and lineage. The review summarizes the present status of aquatic invertebrates identification and the importance of these two techniques in the taxonomic identification of aquatic insects. Elsevier 2022-12-12 /pmc/articles/PMC9758433/ /pubmed/36536920 http://dx.doi.org/10.1016/j.heliyon.2022.e12236 Text en © 2022 The Author(s) https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Review Article
Grover, Aseem
Sharma, Parul
Sharma, Radhika
Sinha, Reshma
Ultrastructural and molecular approach as a tool for taxonomic identification of aquatic macroinvertebrates: A review
title Ultrastructural and molecular approach as a tool for taxonomic identification of aquatic macroinvertebrates: A review
title_full Ultrastructural and molecular approach as a tool for taxonomic identification of aquatic macroinvertebrates: A review
title_fullStr Ultrastructural and molecular approach as a tool for taxonomic identification of aquatic macroinvertebrates: A review
title_full_unstemmed Ultrastructural and molecular approach as a tool for taxonomic identification of aquatic macroinvertebrates: A review
title_short Ultrastructural and molecular approach as a tool for taxonomic identification of aquatic macroinvertebrates: A review
title_sort ultrastructural and molecular approach as a tool for taxonomic identification of aquatic macroinvertebrates: a review
topic Review Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9758433/
https://www.ncbi.nlm.nih.gov/pubmed/36536920
http://dx.doi.org/10.1016/j.heliyon.2022.e12236
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