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Chemical component analysis of the traditional Chinese medicine Guipi Tang and its effects on major depressive disorder at molecular level

ETHNOPHARMACOLOGICAL RELEVANCE: Guipi Tang (GPT) is a widely used traditional Chinese medicine that is used to treat major depressive disorder. However, the molecular mechanisms of its effects remain unclear. AIM OF THE STUDY: This study aimed to investigate the antidepressant-like effects of GPT an...

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Autores principales: Li, Tingting, Li, Xiangting, Zhang, Jingsi, Yu, Zhonghai, Gong, Fan, Wang, Jun, Tang, Haiyan, Xiang, Jun, Zhang, Wen, Cai, Dingfang
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9758438/
https://www.ncbi.nlm.nih.gov/pubmed/36536902
http://dx.doi.org/10.1016/j.heliyon.2022.e12182
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author Li, Tingting
Li, Xiangting
Zhang, Jingsi
Yu, Zhonghai
Gong, Fan
Wang, Jun
Tang, Haiyan
Xiang, Jun
Zhang, Wen
Cai, Dingfang
author_facet Li, Tingting
Li, Xiangting
Zhang, Jingsi
Yu, Zhonghai
Gong, Fan
Wang, Jun
Tang, Haiyan
Xiang, Jun
Zhang, Wen
Cai, Dingfang
author_sort Li, Tingting
collection PubMed
description ETHNOPHARMACOLOGICAL RELEVANCE: Guipi Tang (GPT) is a widely used traditional Chinese medicine that is used to treat major depressive disorder. However, the molecular mechanisms of its effects remain unclear. AIM OF THE STUDY: This study aimed to investigate the antidepressant-like effects of GPT and explore its underlying molecular mechanisms. MATERIALS AND METHODS: Male Sprague–Dawley rats were subjected to a chronic unpredictable mild stress (CUMS) procedure and treated with various doses of GPT, with fluoxetine treatment as a positive control. Behavioural tests (including sucrose preference test, novelty-suppressed feeding test, open-field test and forced swim test), terminal deoxynucleotidyl transferase dUTP nick end labeling and enzyme-linked immunosorbent assay were conducted. The levels of Bax, Bcl-2, cleaved caspase-3, PI3K, p-PI3K, AKT, p-AKT, BDNF, TrkB and CREB or p-CREB were assessed at the protein level using western blotting or immunofluorescence. RESULTS: GPT consists of mainly known drugs, such as liquiritin and ginsenosides. It reversed depressive behaviours and decreased cell apoptosis in the hippocampi of CUMS rats. It significantly upregulated the protein level of Bax, p-Akt, p-PI3K, BDNF, TrkB and p-CREB and downregulated the level of cleaved caspase-3 and Bcl-2. CONCLUSIONS: GPT had anti-depressive activity as indicated by the amelioration of depression-like behaviour and the inhibition of hippocampal neuronal apoptosis in CUMS rats. This inhibition was mediated partly by modulating the PI3K/Akt and/or BDNF/TrkB/CREB pathway, in which, glycosides, the main components of GPT, might be involved.
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spelling pubmed-97584382022-12-18 Chemical component analysis of the traditional Chinese medicine Guipi Tang and its effects on major depressive disorder at molecular level Li, Tingting Li, Xiangting Zhang, Jingsi Yu, Zhonghai Gong, Fan Wang, Jun Tang, Haiyan Xiang, Jun Zhang, Wen Cai, Dingfang Heliyon Research Article ETHNOPHARMACOLOGICAL RELEVANCE: Guipi Tang (GPT) is a widely used traditional Chinese medicine that is used to treat major depressive disorder. However, the molecular mechanisms of its effects remain unclear. AIM OF THE STUDY: This study aimed to investigate the antidepressant-like effects of GPT and explore its underlying molecular mechanisms. MATERIALS AND METHODS: Male Sprague–Dawley rats were subjected to a chronic unpredictable mild stress (CUMS) procedure and treated with various doses of GPT, with fluoxetine treatment as a positive control. Behavioural tests (including sucrose preference test, novelty-suppressed feeding test, open-field test and forced swim test), terminal deoxynucleotidyl transferase dUTP nick end labeling and enzyme-linked immunosorbent assay were conducted. The levels of Bax, Bcl-2, cleaved caspase-3, PI3K, p-PI3K, AKT, p-AKT, BDNF, TrkB and CREB or p-CREB were assessed at the protein level using western blotting or immunofluorescence. RESULTS: GPT consists of mainly known drugs, such as liquiritin and ginsenosides. It reversed depressive behaviours and decreased cell apoptosis in the hippocampi of CUMS rats. It significantly upregulated the protein level of Bax, p-Akt, p-PI3K, BDNF, TrkB and p-CREB and downregulated the level of cleaved caspase-3 and Bcl-2. CONCLUSIONS: GPT had anti-depressive activity as indicated by the amelioration of depression-like behaviour and the inhibition of hippocampal neuronal apoptosis in CUMS rats. This inhibition was mediated partly by modulating the PI3K/Akt and/or BDNF/TrkB/CREB pathway, in which, glycosides, the main components of GPT, might be involved. Elsevier 2022-12-09 /pmc/articles/PMC9758438/ /pubmed/36536902 http://dx.doi.org/10.1016/j.heliyon.2022.e12182 Text en © 2022 The Author(s) https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Research Article
Li, Tingting
Li, Xiangting
Zhang, Jingsi
Yu, Zhonghai
Gong, Fan
Wang, Jun
Tang, Haiyan
Xiang, Jun
Zhang, Wen
Cai, Dingfang
Chemical component analysis of the traditional Chinese medicine Guipi Tang and its effects on major depressive disorder at molecular level
title Chemical component analysis of the traditional Chinese medicine Guipi Tang and its effects on major depressive disorder at molecular level
title_full Chemical component analysis of the traditional Chinese medicine Guipi Tang and its effects on major depressive disorder at molecular level
title_fullStr Chemical component analysis of the traditional Chinese medicine Guipi Tang and its effects on major depressive disorder at molecular level
title_full_unstemmed Chemical component analysis of the traditional Chinese medicine Guipi Tang and its effects on major depressive disorder at molecular level
title_short Chemical component analysis of the traditional Chinese medicine Guipi Tang and its effects on major depressive disorder at molecular level
title_sort chemical component analysis of the traditional chinese medicine guipi tang and its effects on major depressive disorder at molecular level
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9758438/
https://www.ncbi.nlm.nih.gov/pubmed/36536902
http://dx.doi.org/10.1016/j.heliyon.2022.e12182
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