An updated protocol for in vitro bovine embryo production

Cattle embryos represent a useful model for understanding parts of human embryogenesis due to various biological similarities. We describe a protocol to mature and fertilize bovine oocytes followed by culture of resulting presumptive zygotes up until the blastocyst stage. Our protocol features a uni...

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Detalles Bibliográficos
Autores principales: Speckhart, Savannah L., Wooldridge, Lydia K., Ealy, Alan D.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2022
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9758491/
https://www.ncbi.nlm.nih.gov/pubmed/36520625
http://dx.doi.org/10.1016/j.xpro.2022.101924
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author Speckhart, Savannah L.
Wooldridge, Lydia K.
Ealy, Alan D.
author_facet Speckhart, Savannah L.
Wooldridge, Lydia K.
Ealy, Alan D.
author_sort Speckhart, Savannah L.
collection PubMed
description Cattle embryos represent a useful model for understanding parts of human embryogenesis due to various biological similarities. We describe a protocol to mature and fertilize bovine oocytes followed by culture of resulting presumptive zygotes up until the blastocyst stage. Our protocol features a unique procedure for washing and moving oocytes and zygotes between their respective dishes using a cell strainer. A thorough troubleshooting section will help users optimize embryo development with cleavage and blastocyst rates exceeding 70% and 20%, respectively. For complete details on the use and execution of this protocol, please refer to Wooldridge and Ealy (2019).(1)
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spelling pubmed-97584912022-12-18 An updated protocol for in vitro bovine embryo production Speckhart, Savannah L. Wooldridge, Lydia K. Ealy, Alan D. STAR Protoc Protocol Cattle embryos represent a useful model for understanding parts of human embryogenesis due to various biological similarities. We describe a protocol to mature and fertilize bovine oocytes followed by culture of resulting presumptive zygotes up until the blastocyst stage. Our protocol features a unique procedure for washing and moving oocytes and zygotes between their respective dishes using a cell strainer. A thorough troubleshooting section will help users optimize embryo development with cleavage and blastocyst rates exceeding 70% and 20%, respectively. For complete details on the use and execution of this protocol, please refer to Wooldridge and Ealy (2019).(1) Elsevier 2022-12-13 /pmc/articles/PMC9758491/ /pubmed/36520625 http://dx.doi.org/10.1016/j.xpro.2022.101924 Text en © 2022 The Authors https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Protocol
Speckhart, Savannah L.
Wooldridge, Lydia K.
Ealy, Alan D.
An updated protocol for in vitro bovine embryo production
title An updated protocol for in vitro bovine embryo production
title_full An updated protocol for in vitro bovine embryo production
title_fullStr An updated protocol for in vitro bovine embryo production
title_full_unstemmed An updated protocol for in vitro bovine embryo production
title_short An updated protocol for in vitro bovine embryo production
title_sort updated protocol for in vitro bovine embryo production
topic Protocol
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9758491/
https://www.ncbi.nlm.nih.gov/pubmed/36520625
http://dx.doi.org/10.1016/j.xpro.2022.101924
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