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Streamlined high-throughput cloning protocol to generate arrayed mutant libraries
Large-scale, site-directed mutagenesis enables rapid characterization of the biochemical and biological properties of proteins. Here, we present a cost-effective and adaptable cloning pipeline to generate arrayed gene libraries for a construct of interest. We detail steps to use an open access web a...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9758493/ https://www.ncbi.nlm.nih.gov/pubmed/36520626 http://dx.doi.org/10.1016/j.xpro.2022.101930 |