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Equipment-free, salt-mediated immobilization of nucleic acids for nucleic acid lateral flow assays
As the world has been facing several deadly virus crises, including Zika virus disease, Ebola virus disease, severe acute respiratory syndrome (SARS), Middle East respiratory syndrome (MERS), and Coronavirus disease 2019 (COVID-19), lateral flow assays (LFAs), which require minimal equipment for poi...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier B.V.
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9758654/ https://www.ncbi.nlm.nih.gov/pubmed/36568876 http://dx.doi.org/10.1016/j.snb.2021.130975 |
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author | Park, Jung Soo Kim, Seokjoon Han, Jinjoo Kim, Jung Ho Park, Ki Soo |
author_facet | Park, Jung Soo Kim, Seokjoon Han, Jinjoo Kim, Jung Ho Park, Ki Soo |
author_sort | Park, Jung Soo |
collection | PubMed |
description | As the world has been facing several deadly virus crises, including Zika virus disease, Ebola virus disease, severe acute respiratory syndrome (SARS), Middle East respiratory syndrome (MERS), and Coronavirus disease 2019 (COVID-19), lateral flow assays (LFAs), which require minimal equipment for point-of-care of viral infectious diseases, are garnering much attention. Accordingly, there is an increasing demand to reduce the time and cost required for manufacturing LFAs. The current study introduces an equipment-free method of salt-mediated immobilization of nucleic acids (SAIoNs) for LFAs. Compared to general DNA immobilization methods such as streptavidin–biotin, UV-irradiation, and heat treatment, our method does not require special equipment (e.g., centrifuge, UV-crosslinker, heating device); therefore, it can be applied in a resource-limited environment with reduced production costs. The immobilization process was streamlined and completed within 30 min. Our method improved the color intensity signal approximately 14 times compared to the method without using SAIoNs and exhibited reproducibility with the long-term storage stability. The proposed method can be used to detect practical targets (e.g., SARS-CoV-2) and facilitates highly sensitive and selective detection of target nucleic acids with multiplexing capability and without any cross-reactivity. This novel immobilization strategy provides a basis for easily and inexpensively developing nucleic acid LFAs combined with various types of nucleic acid amplification. |
format | Online Article Text |
id | pubmed-9758654 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Elsevier B.V. |
record_format | MEDLINE/PubMed |
spelling | pubmed-97586542022-12-19 Equipment-free, salt-mediated immobilization of nucleic acids for nucleic acid lateral flow assays Park, Jung Soo Kim, Seokjoon Han, Jinjoo Kim, Jung Ho Park, Ki Soo Sens Actuators B Chem Article As the world has been facing several deadly virus crises, including Zika virus disease, Ebola virus disease, severe acute respiratory syndrome (SARS), Middle East respiratory syndrome (MERS), and Coronavirus disease 2019 (COVID-19), lateral flow assays (LFAs), which require minimal equipment for point-of-care of viral infectious diseases, are garnering much attention. Accordingly, there is an increasing demand to reduce the time and cost required for manufacturing LFAs. The current study introduces an equipment-free method of salt-mediated immobilization of nucleic acids (SAIoNs) for LFAs. Compared to general DNA immobilization methods such as streptavidin–biotin, UV-irradiation, and heat treatment, our method does not require special equipment (e.g., centrifuge, UV-crosslinker, heating device); therefore, it can be applied in a resource-limited environment with reduced production costs. The immobilization process was streamlined and completed within 30 min. Our method improved the color intensity signal approximately 14 times compared to the method without using SAIoNs and exhibited reproducibility with the long-term storage stability. The proposed method can be used to detect practical targets (e.g., SARS-CoV-2) and facilitates highly sensitive and selective detection of target nucleic acids with multiplexing capability and without any cross-reactivity. This novel immobilization strategy provides a basis for easily and inexpensively developing nucleic acid LFAs combined with various types of nucleic acid amplification. Elsevier B.V. 2022-01-15 2021-10-23 /pmc/articles/PMC9758654/ /pubmed/36568876 http://dx.doi.org/10.1016/j.snb.2021.130975 Text en © 2021 Elsevier B.V. All rights reserved. Since January 2020 Elsevier has created a COVID-19 resource centre with free information in English and Mandarin on the novel coronavirus COVID-19. The COVID-19 resource centre is hosted on Elsevier Connect, the company's public news and information website. Elsevier hereby grants permission to make all its COVID-19-related research that is available on the COVID-19 resource centre - including this research content - immediately available in PubMed Central and other publicly funded repositories, such as the WHO COVID database with rights for unrestricted research re-use and analyses in any form or by any means with acknowledgement of the original source. These permissions are granted for free by Elsevier for as long as the COVID-19 resource centre remains active. |
spellingShingle | Article Park, Jung Soo Kim, Seokjoon Han, Jinjoo Kim, Jung Ho Park, Ki Soo Equipment-free, salt-mediated immobilization of nucleic acids for nucleic acid lateral flow assays |
title | Equipment-free, salt-mediated immobilization of nucleic acids for nucleic acid lateral flow assays |
title_full | Equipment-free, salt-mediated immobilization of nucleic acids for nucleic acid lateral flow assays |
title_fullStr | Equipment-free, salt-mediated immobilization of nucleic acids for nucleic acid lateral flow assays |
title_full_unstemmed | Equipment-free, salt-mediated immobilization of nucleic acids for nucleic acid lateral flow assays |
title_short | Equipment-free, salt-mediated immobilization of nucleic acids for nucleic acid lateral flow assays |
title_sort | equipment-free, salt-mediated immobilization of nucleic acids for nucleic acid lateral flow assays |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9758654/ https://www.ncbi.nlm.nih.gov/pubmed/36568876 http://dx.doi.org/10.1016/j.snb.2021.130975 |
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