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Metabonomic Analysis of Metabolites Produced by Escherichia coli in Patients With and Without Sepsis

AIM: To analyze the metabolites of the most common sepsis-related pathogen and their correlation with clinical indicators. METHODS: Information of bacterial-infection patients in Huzhou Central hospital was retrospectively investigated and analyzed. The most common pathogen inducing sepsis was selec...

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Autores principales: Wang, Yangyanqiu, Jin, Yin, Ji, Xiaowei, Huang, Man, Xie, Bo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Dove 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9759013/
https://www.ncbi.nlm.nih.gov/pubmed/36536860
http://dx.doi.org/10.2147/IDR.S388034
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author Wang, Yangyanqiu
Jin, Yin
Ji, Xiaowei
Huang, Man
Xie, Bo
author_facet Wang, Yangyanqiu
Jin, Yin
Ji, Xiaowei
Huang, Man
Xie, Bo
author_sort Wang, Yangyanqiu
collection PubMed
description AIM: To analyze the metabolites of the most common sepsis-related pathogen and their correlation with clinical indicators. METHODS: Information of bacterial-infection patients in Huzhou Central hospital was retrospectively investigated and analyzed. The most common pathogen inducing sepsis was selected. Then, the metabolic profiles of pathogens from blood were detected by liquid chromatography/mass spectrometry. Cluster and classification analysis, KEGG pathway enrichment analysis, multidimensional OPLS-DA, Z scores, correlation analysis were used to analyze the metabolites. RESULTS: Escherichia coli (E. coli) was the pathogen that caused the most infection (about 21%) and sepsis. Amino acids, peptides, terpene glycosides, carbohydrates were the main metabolites of E.coli and they were mainly digestive and endocrine-related compounds. Most of them were related to amino acids metabolism, cofactors and vitamins metabolism, biosynthesis of secondary metabolites, et al. Moreover, metabolites were involved in purine metabolism, neuroactive ligand-receptor interaction, ABC transporters, etc. Then, over 70 differential metabolites such as tyramine, tryptophan, 3- hydroxymalondialdehyde were screened in E.coli from nonseptic and septic patients. They were mainly involved in phenylalanine metabolism, tryptophan metabolism, protein digestion and absorption. Distribution of metabolites of E. coli from nonseptic and septic patients was obviously different. What is more, differential metabolites had evidently correlation with SOFA score, APPACHE II score, C-reactive protein, erythrocyte, platelet, aspartate aminotransferase, coagulation function, lactic acid (p < 0.01). CONCLUSION: The different metabolic profile of E. coli from nonseptic and septic patients indicated that differential metabolites might be associated with sepsis.
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spelling pubmed-97590132022-12-18 Metabonomic Analysis of Metabolites Produced by Escherichia coli in Patients With and Without Sepsis Wang, Yangyanqiu Jin, Yin Ji, Xiaowei Huang, Man Xie, Bo Infect Drug Resist Original Research AIM: To analyze the metabolites of the most common sepsis-related pathogen and their correlation with clinical indicators. METHODS: Information of bacterial-infection patients in Huzhou Central hospital was retrospectively investigated and analyzed. The most common pathogen inducing sepsis was selected. Then, the metabolic profiles of pathogens from blood were detected by liquid chromatography/mass spectrometry. Cluster and classification analysis, KEGG pathway enrichment analysis, multidimensional OPLS-DA, Z scores, correlation analysis were used to analyze the metabolites. RESULTS: Escherichia coli (E. coli) was the pathogen that caused the most infection (about 21%) and sepsis. Amino acids, peptides, terpene glycosides, carbohydrates were the main metabolites of E.coli and they were mainly digestive and endocrine-related compounds. Most of them were related to amino acids metabolism, cofactors and vitamins metabolism, biosynthesis of secondary metabolites, et al. Moreover, metabolites were involved in purine metabolism, neuroactive ligand-receptor interaction, ABC transporters, etc. Then, over 70 differential metabolites such as tyramine, tryptophan, 3- hydroxymalondialdehyde were screened in E.coli from nonseptic and septic patients. They were mainly involved in phenylalanine metabolism, tryptophan metabolism, protein digestion and absorption. Distribution of metabolites of E. coli from nonseptic and septic patients was obviously different. What is more, differential metabolites had evidently correlation with SOFA score, APPACHE II score, C-reactive protein, erythrocyte, platelet, aspartate aminotransferase, coagulation function, lactic acid (p < 0.01). CONCLUSION: The different metabolic profile of E. coli from nonseptic and septic patients indicated that differential metabolites might be associated with sepsis. Dove 2022-12-13 /pmc/articles/PMC9759013/ /pubmed/36536860 http://dx.doi.org/10.2147/IDR.S388034 Text en © 2022 Wang et al. https://creativecommons.org/licenses/by-nc/3.0/This work is published and licensed by Dove Medical Press Limited. The full terms of this license are available at https://www.dovepress.com/terms.php and incorporate the Creative Commons Attribution – Non Commercial (unported, v3.0) License (http://creativecommons.org/licenses/by-nc/3.0/ (https://creativecommons.org/licenses/by-nc/3.0/) ). By accessing the work you hereby accept the Terms. Non-commercial uses of the work are permitted without any further permission from Dove Medical Press Limited, provided the work is properly attributed. For permission for commercial use of this work, please see paragraphs 4.2 and 5 of our Terms (https://www.dovepress.com/terms.php).
spellingShingle Original Research
Wang, Yangyanqiu
Jin, Yin
Ji, Xiaowei
Huang, Man
Xie, Bo
Metabonomic Analysis of Metabolites Produced by Escherichia coli in Patients With and Without Sepsis
title Metabonomic Analysis of Metabolites Produced by Escherichia coli in Patients With and Without Sepsis
title_full Metabonomic Analysis of Metabolites Produced by Escherichia coli in Patients With and Without Sepsis
title_fullStr Metabonomic Analysis of Metabolites Produced by Escherichia coli in Patients With and Without Sepsis
title_full_unstemmed Metabonomic Analysis of Metabolites Produced by Escherichia coli in Patients With and Without Sepsis
title_short Metabonomic Analysis of Metabolites Produced by Escherichia coli in Patients With and Without Sepsis
title_sort metabonomic analysis of metabolites produced by escherichia coli in patients with and without sepsis
topic Original Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9759013/
https://www.ncbi.nlm.nih.gov/pubmed/36536860
http://dx.doi.org/10.2147/IDR.S388034
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