Inheritance of 2,4-dichlorophenoxyacetic acid (2,4-D) resistance in Amaranthus palmeri

In this study, the inheritance of 2,4-D resistance in a multiple herbicide-resistant Palmer amaranth (KCTR) was investigated. Direct and reciprocal crosses were performed using 2,4-D-resistant KCTR and susceptible KSS plants to generate F(1) progenies. 2,4-D dose–response assays were conducted to ev...

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Autores principales: Shyam, Chandrima, Peterson, Dallas E., Jhala, Amit J., Jugulam, Mithila
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9759536/
https://www.ncbi.nlm.nih.gov/pubmed/36528649
http://dx.doi.org/10.1038/s41598-022-25686-1
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author Shyam, Chandrima
Peterson, Dallas E.
Jhala, Amit J.
Jugulam, Mithila
author_facet Shyam, Chandrima
Peterson, Dallas E.
Jhala, Amit J.
Jugulam, Mithila
author_sort Shyam, Chandrima
collection PubMed
description In this study, the inheritance of 2,4-D resistance in a multiple herbicide-resistant Palmer amaranth (KCTR) was investigated. Direct and reciprocal crosses were performed using 2,4-D-resistant KCTR and susceptible KSS plants to generate F(1) progenies. 2,4-D dose–response assays were conducted to evaluate the response of progenies from each F(1) family along with KCTR and KSS plants in controlled environmental growth chambers. Additionally, 2,4-D-resistant male and female plants from each of the F(1) families were used in pairwise crosses to generate pseudo-F(2) families. Segregation (resistance or susceptibility) of progenies from the F(2) families in response to a discriminatory rate of 2,4-D (i.e., 560 g ae ha(−1)) was evaluated. Dose–response analysis of F(1) progenies derived from direct and reciprocal crosses suggested that the 2,4-D resistance in KCTR is a nuclear trait. Chi-square analyses of F(2) segregation data implied that 2,4-D resistance in KCTR is controlled by multiple gene(s). Overall, our data suggest that the 2,4-D resistance in KCTR Palmer amaranth is a nuclear inherited trait controlled by multiple genes. Such resistance can spread both via pollen or seed-mediated gene flow. In future, efforts will be directed towards identifying genes mediating 2,4-D resistance in KCTR population.
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spelling pubmed-97595362022-12-19 Inheritance of 2,4-dichlorophenoxyacetic acid (2,4-D) resistance in Amaranthus palmeri Shyam, Chandrima Peterson, Dallas E. Jhala, Amit J. Jugulam, Mithila Sci Rep Article In this study, the inheritance of 2,4-D resistance in a multiple herbicide-resistant Palmer amaranth (KCTR) was investigated. Direct and reciprocal crosses were performed using 2,4-D-resistant KCTR and susceptible KSS plants to generate F(1) progenies. 2,4-D dose–response assays were conducted to evaluate the response of progenies from each F(1) family along with KCTR and KSS plants in controlled environmental growth chambers. Additionally, 2,4-D-resistant male and female plants from each of the F(1) families were used in pairwise crosses to generate pseudo-F(2) families. Segregation (resistance or susceptibility) of progenies from the F(2) families in response to a discriminatory rate of 2,4-D (i.e., 560 g ae ha(−1)) was evaluated. Dose–response analysis of F(1) progenies derived from direct and reciprocal crosses suggested that the 2,4-D resistance in KCTR is a nuclear trait. Chi-square analyses of F(2) segregation data implied that 2,4-D resistance in KCTR is controlled by multiple gene(s). Overall, our data suggest that the 2,4-D resistance in KCTR Palmer amaranth is a nuclear inherited trait controlled by multiple genes. Such resistance can spread both via pollen or seed-mediated gene flow. In future, efforts will be directed towards identifying genes mediating 2,4-D resistance in KCTR population. Nature Publishing Group UK 2022-12-17 /pmc/articles/PMC9759536/ /pubmed/36528649 http://dx.doi.org/10.1038/s41598-022-25686-1 Text en © The Author(s) 2022 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Article
Shyam, Chandrima
Peterson, Dallas E.
Jhala, Amit J.
Jugulam, Mithila
Inheritance of 2,4-dichlorophenoxyacetic acid (2,4-D) resistance in Amaranthus palmeri
title Inheritance of 2,4-dichlorophenoxyacetic acid (2,4-D) resistance in Amaranthus palmeri
title_full Inheritance of 2,4-dichlorophenoxyacetic acid (2,4-D) resistance in Amaranthus palmeri
title_fullStr Inheritance of 2,4-dichlorophenoxyacetic acid (2,4-D) resistance in Amaranthus palmeri
title_full_unstemmed Inheritance of 2,4-dichlorophenoxyacetic acid (2,4-D) resistance in Amaranthus palmeri
title_short Inheritance of 2,4-dichlorophenoxyacetic acid (2,4-D) resistance in Amaranthus palmeri
title_sort inheritance of 2,4-dichlorophenoxyacetic acid (2,4-d) resistance in amaranthus palmeri
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9759536/
https://www.ncbi.nlm.nih.gov/pubmed/36528649
http://dx.doi.org/10.1038/s41598-022-25686-1
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