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Nesfatin-1 Stimulates CCL2-dependent Monocyte Migration And M1 Macrophage Polarization: Implications For Rheumatoid Arthritis Therapy

Rheumatoid arthritis (RA) is a prototypic inflammatory disease, characterized by the infiltration of proinflammatory cytokines into the joint synovium and the migration of mononuclear cells into inflammatory sites. The adipokine nesfatin-1 is linked to inflammatory events in various diseases, althou...

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Autores principales: Chang, Jun-Way, Liu, Shan-Chi, Lin, Yen-You, He, Xiu-Yuan, Wu, Yi-Syuan, Su, Chen-Ming, Tsai, Chun-Hao, Chen, Hsien-Te, Fong, Yi-Ching, Hu, Sung-Lin, Huang, Chien-Chung, Tang, Chih-Hsin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Ivyspring International Publisher 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9760434/
https://www.ncbi.nlm.nih.gov/pubmed/36594093
http://dx.doi.org/10.7150/ijbs.77987
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author Chang, Jun-Way
Liu, Shan-Chi
Lin, Yen-You
He, Xiu-Yuan
Wu, Yi-Syuan
Su, Chen-Ming
Tsai, Chun-Hao
Chen, Hsien-Te
Fong, Yi-Ching
Hu, Sung-Lin
Huang, Chien-Chung
Tang, Chih-Hsin
author_facet Chang, Jun-Way
Liu, Shan-Chi
Lin, Yen-You
He, Xiu-Yuan
Wu, Yi-Syuan
Su, Chen-Ming
Tsai, Chun-Hao
Chen, Hsien-Te
Fong, Yi-Ching
Hu, Sung-Lin
Huang, Chien-Chung
Tang, Chih-Hsin
author_sort Chang, Jun-Way
collection PubMed
description Rheumatoid arthritis (RA) is a prototypic inflammatory disease, characterized by the infiltration of proinflammatory cytokines into the joint synovium and the migration of mononuclear cells into inflammatory sites. The adipokine nesfatin-1 is linked to inflammatory events in various diseases, although its role in RA pathology is uncertain. Analysis of the Gene Expression Omnibus GSE55235 dataset revealed high levels of expression of the adipokine nesfatin-1 in human RA synovial tissue. Similarly, our human synovial tissue samples exhibited increasing levels of nesfatin-1 expression and Ccl2 mRNA expression. Nesfatin-1-induced stimulation of CCL2 expression and monocyte migration involved the MEK/ERK, p38, and NF-κB signaling pathways. Notably, nesfatin-1-induced increases in CCL2 expression favored M1 macrophage polarization, which increased the expression of proinflammatory cytokines IL-1β, IL-6, and TNF-α. Finally, nesfatin-1 shRNA ameliorated the severity of inflammatory disease and reduced levels of M1 macrophage expression in CIA mice. Our studies confirm that nesfatin-1 appears to be worth targeting in RA treatment.
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spelling pubmed-97604342023-01-01 Nesfatin-1 Stimulates CCL2-dependent Monocyte Migration And M1 Macrophage Polarization: Implications For Rheumatoid Arthritis Therapy Chang, Jun-Way Liu, Shan-Chi Lin, Yen-You He, Xiu-Yuan Wu, Yi-Syuan Su, Chen-Ming Tsai, Chun-Hao Chen, Hsien-Te Fong, Yi-Ching Hu, Sung-Lin Huang, Chien-Chung Tang, Chih-Hsin Int J Biol Sci Research Paper Rheumatoid arthritis (RA) is a prototypic inflammatory disease, characterized by the infiltration of proinflammatory cytokines into the joint synovium and the migration of mononuclear cells into inflammatory sites. The adipokine nesfatin-1 is linked to inflammatory events in various diseases, although its role in RA pathology is uncertain. Analysis of the Gene Expression Omnibus GSE55235 dataset revealed high levels of expression of the adipokine nesfatin-1 in human RA synovial tissue. Similarly, our human synovial tissue samples exhibited increasing levels of nesfatin-1 expression and Ccl2 mRNA expression. Nesfatin-1-induced stimulation of CCL2 expression and monocyte migration involved the MEK/ERK, p38, and NF-κB signaling pathways. Notably, nesfatin-1-induced increases in CCL2 expression favored M1 macrophage polarization, which increased the expression of proinflammatory cytokines IL-1β, IL-6, and TNF-α. Finally, nesfatin-1 shRNA ameliorated the severity of inflammatory disease and reduced levels of M1 macrophage expression in CIA mice. Our studies confirm that nesfatin-1 appears to be worth targeting in RA treatment. Ivyspring International Publisher 2023-01-01 /pmc/articles/PMC9760434/ /pubmed/36594093 http://dx.doi.org/10.7150/ijbs.77987 Text en © The author(s) https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/). See http://ivyspring.com/terms for full terms and conditions.
spellingShingle Research Paper
Chang, Jun-Way
Liu, Shan-Chi
Lin, Yen-You
He, Xiu-Yuan
Wu, Yi-Syuan
Su, Chen-Ming
Tsai, Chun-Hao
Chen, Hsien-Te
Fong, Yi-Ching
Hu, Sung-Lin
Huang, Chien-Chung
Tang, Chih-Hsin
Nesfatin-1 Stimulates CCL2-dependent Monocyte Migration And M1 Macrophage Polarization: Implications For Rheumatoid Arthritis Therapy
title Nesfatin-1 Stimulates CCL2-dependent Monocyte Migration And M1 Macrophage Polarization: Implications For Rheumatoid Arthritis Therapy
title_full Nesfatin-1 Stimulates CCL2-dependent Monocyte Migration And M1 Macrophage Polarization: Implications For Rheumatoid Arthritis Therapy
title_fullStr Nesfatin-1 Stimulates CCL2-dependent Monocyte Migration And M1 Macrophage Polarization: Implications For Rheumatoid Arthritis Therapy
title_full_unstemmed Nesfatin-1 Stimulates CCL2-dependent Monocyte Migration And M1 Macrophage Polarization: Implications For Rheumatoid Arthritis Therapy
title_short Nesfatin-1 Stimulates CCL2-dependent Monocyte Migration And M1 Macrophage Polarization: Implications For Rheumatoid Arthritis Therapy
title_sort nesfatin-1 stimulates ccl2-dependent monocyte migration and m1 macrophage polarization: implications for rheumatoid arthritis therapy
topic Research Paper
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9760434/
https://www.ncbi.nlm.nih.gov/pubmed/36594093
http://dx.doi.org/10.7150/ijbs.77987
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