Cargando…

Arachidonic acid is associated with dyslipidemia and cholesterol-related lipoprotein metabolism signatures

INTRODUCTION: Abnormal lipoprotein metabolism is associated with a variety of diseases, cardiovascular disease in particular. Free fatty acids (FAs) and triglycerides (TGs) are the principal lipid species in adipocytes and are the major components of lipoproteins. However, in routine clinical labora...

Descripción completa

Detalles Bibliográficos
Autores principales: Li, Fan, Wang, Yu, Yu, Huahui, Gao, Xiaoqian, Li, Linyi, Sun, Haili, Qin, Yanwen
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9760855/
https://www.ncbi.nlm.nih.gov/pubmed/36545018
http://dx.doi.org/10.3389/fcvm.2022.1075421
_version_ 1784852574501863424
author Li, Fan
Wang, Yu
Yu, Huahui
Gao, Xiaoqian
Li, Linyi
Sun, Haili
Qin, Yanwen
author_facet Li, Fan
Wang, Yu
Yu, Huahui
Gao, Xiaoqian
Li, Linyi
Sun, Haili
Qin, Yanwen
author_sort Li, Fan
collection PubMed
description INTRODUCTION: Abnormal lipoprotein metabolism is associated with a variety of diseases, cardiovascular disease in particular. Free fatty acids (FAs) and triglycerides (TGs) are the principal lipid species in adipocytes and are the major components of lipoproteins. However, in routine clinical laboratory testing, only the total plasma concentrations of FAs and TGs are typically measured. METHODS: We collected 965 individuals with hyperlipidemia plasma and clinical characteristics; high-throughput metabolomics permits the accurate qualitative and quantitative assessment of a variety of specific FAs and TGs and their association with lipoproteins; through regression analysis, the correlation between multiple metabolites and routine measured lipid parameters was found. Mice were fed a diet containing AA, and the concentrations of TC and TG in the plasma of mice were detected by enzyme method, western blot and qRT-PCR detected the protein and mRNA levels of cholesterol synthesis and metabolism in mice. RESULT: Using LC-MS/MS identified eight free FA and 27 TG species in plasma samples, the plasma concentrations of free arachidonic acid (AA) and AA-enriched TG species were significantly associated with the plasma low-density lipoprotein-cholesterol, apolipoprotein B (ApoB), and total cholesterol (TC) concentrations after adjustment for age, sex, the use of lipid-lowering therapy, and body mass index. AA-rich diet significantly increased the plasma concentrations of TC and ApoB and the liver expression of ApoB protein and reduced the protein expression of ATP binding cassette subfamily G members 5 and 8 in mice. DISCUSSION: In this study, it was clarified that the plasma concentrations of free AA- and AA-enriched TG species were significantly associated with the plasma low-density lipoprotein-cholesterol, ApoB, and TC concentrations in individuals with hyperlipidemia, and it was verified that AA could increase the plasma TC level in mice. Taken together, these findings suggest a potential role of AA in the regulation of plasma cholesterol and lipoprotein concentrations.
format Online
Article
Text
id pubmed-9760855
institution National Center for Biotechnology Information
language English
publishDate 2022
publisher Frontiers Media S.A.
record_format MEDLINE/PubMed
spelling pubmed-97608552022-12-20 Arachidonic acid is associated with dyslipidemia and cholesterol-related lipoprotein metabolism signatures Li, Fan Wang, Yu Yu, Huahui Gao, Xiaoqian Li, Linyi Sun, Haili Qin, Yanwen Front Cardiovasc Med Cardiovascular Medicine INTRODUCTION: Abnormal lipoprotein metabolism is associated with a variety of diseases, cardiovascular disease in particular. Free fatty acids (FAs) and triglycerides (TGs) are the principal lipid species in adipocytes and are the major components of lipoproteins. However, in routine clinical laboratory testing, only the total plasma concentrations of FAs and TGs are typically measured. METHODS: We collected 965 individuals with hyperlipidemia plasma and clinical characteristics; high-throughput metabolomics permits the accurate qualitative and quantitative assessment of a variety of specific FAs and TGs and their association with lipoproteins; through regression analysis, the correlation between multiple metabolites and routine measured lipid parameters was found. Mice were fed a diet containing AA, and the concentrations of TC and TG in the plasma of mice were detected by enzyme method, western blot and qRT-PCR detected the protein and mRNA levels of cholesterol synthesis and metabolism in mice. RESULT: Using LC-MS/MS identified eight free FA and 27 TG species in plasma samples, the plasma concentrations of free arachidonic acid (AA) and AA-enriched TG species were significantly associated with the plasma low-density lipoprotein-cholesterol, apolipoprotein B (ApoB), and total cholesterol (TC) concentrations after adjustment for age, sex, the use of lipid-lowering therapy, and body mass index. AA-rich diet significantly increased the plasma concentrations of TC and ApoB and the liver expression of ApoB protein and reduced the protein expression of ATP binding cassette subfamily G members 5 and 8 in mice. DISCUSSION: In this study, it was clarified that the plasma concentrations of free AA- and AA-enriched TG species were significantly associated with the plasma low-density lipoprotein-cholesterol, ApoB, and TC concentrations in individuals with hyperlipidemia, and it was verified that AA could increase the plasma TC level in mice. Taken together, these findings suggest a potential role of AA in the regulation of plasma cholesterol and lipoprotein concentrations. Frontiers Media S.A. 2022-12-05 /pmc/articles/PMC9760855/ /pubmed/36545018 http://dx.doi.org/10.3389/fcvm.2022.1075421 Text en Copyright © 2022 Li, Wang, Yu, Gao, Li, Sun and Qin. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Cardiovascular Medicine
Li, Fan
Wang, Yu
Yu, Huahui
Gao, Xiaoqian
Li, Linyi
Sun, Haili
Qin, Yanwen
Arachidonic acid is associated with dyslipidemia and cholesterol-related lipoprotein metabolism signatures
title Arachidonic acid is associated with dyslipidemia and cholesterol-related lipoprotein metabolism signatures
title_full Arachidonic acid is associated with dyslipidemia and cholesterol-related lipoprotein metabolism signatures
title_fullStr Arachidonic acid is associated with dyslipidemia and cholesterol-related lipoprotein metabolism signatures
title_full_unstemmed Arachidonic acid is associated with dyslipidemia and cholesterol-related lipoprotein metabolism signatures
title_short Arachidonic acid is associated with dyslipidemia and cholesterol-related lipoprotein metabolism signatures
title_sort arachidonic acid is associated with dyslipidemia and cholesterol-related lipoprotein metabolism signatures
topic Cardiovascular Medicine
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9760855/
https://www.ncbi.nlm.nih.gov/pubmed/36545018
http://dx.doi.org/10.3389/fcvm.2022.1075421
work_keys_str_mv AT lifan arachidonicacidisassociatedwithdyslipidemiaandcholesterolrelatedlipoproteinmetabolismsignatures
AT wangyu arachidonicacidisassociatedwithdyslipidemiaandcholesterolrelatedlipoproteinmetabolismsignatures
AT yuhuahui arachidonicacidisassociatedwithdyslipidemiaandcholesterolrelatedlipoproteinmetabolismsignatures
AT gaoxiaoqian arachidonicacidisassociatedwithdyslipidemiaandcholesterolrelatedlipoproteinmetabolismsignatures
AT lilinyi arachidonicacidisassociatedwithdyslipidemiaandcholesterolrelatedlipoproteinmetabolismsignatures
AT sunhaili arachidonicacidisassociatedwithdyslipidemiaandcholesterolrelatedlipoproteinmetabolismsignatures
AT qinyanwen arachidonicacidisassociatedwithdyslipidemiaandcholesterolrelatedlipoproteinmetabolismsignatures