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Spatial variation and metabolic diversity of microbial communities in the surface sediments of the Mariana Trench

Mariana Trench represents the deepest and one of least explored biosphere on Earth, and its carbon sources include euphotic sinking, lateral transportation and diffusion from underlying crust, etc. By far the spatial variation of microbial community with associated organic carbon degradation potenti...

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Autores principales: Wang, Fangzhou, Zhang, Yue, Jing, Hongmei, Liu, Hao
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9760864/
https://www.ncbi.nlm.nih.gov/pubmed/36545198
http://dx.doi.org/10.3389/fmicb.2022.1051999
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author Wang, Fangzhou
Zhang, Yue
Jing, Hongmei
Liu, Hao
author_facet Wang, Fangzhou
Zhang, Yue
Jing, Hongmei
Liu, Hao
author_sort Wang, Fangzhou
collection PubMed
description Mariana Trench represents the deepest and one of least explored biosphere on Earth, and its carbon sources include euphotic sinking, lateral transportation and diffusion from underlying crust, etc. By far the spatial variation of microbial community with associated organic carbon degradation potential in the surface sediments of the Mariana Trench were still largely unknown. Based on the high-throughput 16S rRNA amplicon sequencing, significantly different microbial community structure was overserved between the shallow (<10,000 m) and deep stations (>10,000 m), which could be explained by spatial variation of Chloroflexi, Proteobacteria and Crenarchaeota, with sampling depth and total organic carbon (TOC) content as the environmental driving forces. During the 109-day incubation with Biolog EcoPlate™ microplate, polymers and carbohydrates were preferentially used, followed by amino acids and carboxylic acids, and microbial metabolic diversity was significantly different between the shallow and deep stations. The metabolic diversity of microorganisms at most shallow stations was significantly lower than that at deep stations. This could potentially be attributed the metabolic capabilities of different microbial groups with varied ecological niches, and reflected the initial preference of carbon source by the nature microbes as well. Our study obtained a rough assessment of physiological and taxonomic characteristics of the trench sediment microbial community with polyphasic approaches. Distinct microbial structure and potential carbon metabolic functions in different sampling depths might led to the differentiation of ecological niches, which enable various microorganisms to make full use of the limited resources in the deep sea, and provided a research basis for further exploration of the carbon cycle in different deep-sea regions.
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spelling pubmed-97608642022-12-20 Spatial variation and metabolic diversity of microbial communities in the surface sediments of the Mariana Trench Wang, Fangzhou Zhang, Yue Jing, Hongmei Liu, Hao Front Microbiol Microbiology Mariana Trench represents the deepest and one of least explored biosphere on Earth, and its carbon sources include euphotic sinking, lateral transportation and diffusion from underlying crust, etc. By far the spatial variation of microbial community with associated organic carbon degradation potential in the surface sediments of the Mariana Trench were still largely unknown. Based on the high-throughput 16S rRNA amplicon sequencing, significantly different microbial community structure was overserved between the shallow (<10,000 m) and deep stations (>10,000 m), which could be explained by spatial variation of Chloroflexi, Proteobacteria and Crenarchaeota, with sampling depth and total organic carbon (TOC) content as the environmental driving forces. During the 109-day incubation with Biolog EcoPlate™ microplate, polymers and carbohydrates were preferentially used, followed by amino acids and carboxylic acids, and microbial metabolic diversity was significantly different between the shallow and deep stations. The metabolic diversity of microorganisms at most shallow stations was significantly lower than that at deep stations. This could potentially be attributed the metabolic capabilities of different microbial groups with varied ecological niches, and reflected the initial preference of carbon source by the nature microbes as well. Our study obtained a rough assessment of physiological and taxonomic characteristics of the trench sediment microbial community with polyphasic approaches. Distinct microbial structure and potential carbon metabolic functions in different sampling depths might led to the differentiation of ecological niches, which enable various microorganisms to make full use of the limited resources in the deep sea, and provided a research basis for further exploration of the carbon cycle in different deep-sea regions. Frontiers Media S.A. 2022-12-05 /pmc/articles/PMC9760864/ /pubmed/36545198 http://dx.doi.org/10.3389/fmicb.2022.1051999 Text en Copyright © 2022 Wang, Zhang, Jing and Liu. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Microbiology
Wang, Fangzhou
Zhang, Yue
Jing, Hongmei
Liu, Hao
Spatial variation and metabolic diversity of microbial communities in the surface sediments of the Mariana Trench
title Spatial variation and metabolic diversity of microbial communities in the surface sediments of the Mariana Trench
title_full Spatial variation and metabolic diversity of microbial communities in the surface sediments of the Mariana Trench
title_fullStr Spatial variation and metabolic diversity of microbial communities in the surface sediments of the Mariana Trench
title_full_unstemmed Spatial variation and metabolic diversity of microbial communities in the surface sediments of the Mariana Trench
title_short Spatial variation and metabolic diversity of microbial communities in the surface sediments of the Mariana Trench
title_sort spatial variation and metabolic diversity of microbial communities in the surface sediments of the mariana trench
topic Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9760864/
https://www.ncbi.nlm.nih.gov/pubmed/36545198
http://dx.doi.org/10.3389/fmicb.2022.1051999
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