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Gene-centric coverage of the human liver transcriptome: QPCR, Illumina, and Oxford Nanopore RNA-Seq
It has been shown that the best coverage of the HepG2 cell line transcriptome encoded by genes of a single chromosome, chromosome 18, is achieved by a combination of two sequencing platforms, Illumina RNA-Seq and Oxford Nanopore Technologies (ONT), using cut-off levels of FPKM > 0 and TPM > 0,...
Autores principales: | Ilgisonis, Ekaterina V., Ponomarenko, Elena A., Tarbeeva, Svetlana N., Lisitsa, Andrey V., Zgoda, Victor G., Radko, Sergey P., Archakov, Alexander I. |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Frontiers Media S.A.
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9760921/ https://www.ncbi.nlm.nih.gov/pubmed/36545510 http://dx.doi.org/10.3389/fmolb.2022.944639 |
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