Optimized protocol to generate genome-wide inactivated Cas9-expressing murine T cells

In vivo genome-wide CRISPR screens in primary T cells allow the systematic and unbiased identification of non-redundant regulatory mechanisms shaping immune responses. Here, we present an optimized protocol for efficient generation of a pool of genome-wide inactivated Cas9-expressing T cells using a...

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Detalles Bibliográficos
Autores principales: Laprie-Sentenac, Marguerite, Cretet-Rodeschini, Clara, Menger, Laurie
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2022
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9762184/
https://www.ncbi.nlm.nih.gov/pubmed/36516053
http://dx.doi.org/10.1016/j.xpro.2022.101922

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